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Genetic characterization of Vitis germplasm collected from the southwestern US and Mexico to expedite Pierce’s disease-resistance breeding

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Abstract

Pierce’s disease (PD) limits the cultivation of Vitis vinifera grape cultivars in California, across the southern United States and into South America. Resistance has been well characterized in V. arizonica, and one resistance locus has been identified (PdR1). However, resistance is poorly characterized in most other grape species. We tested a wide range of Vitis species from the southwestern United States for resistance to PD and used nuclear and chloroplast markers to phenotypically and genetically select a diverse set of resistant accessions. Chloroplast SSR markers identified 11 maternal lineage lines within the set of 17 (14 new and three previously identified) PD resistant accessions. A total of 19 breeding populations (F1 and pseudo-BC1) were developed with the 14 PD resistant accessions, and a total of 705 seedlings were analyzed for PD resistance. Using a limited mapping approach, 12 SSR markers, linked to the PdR1 locus, were used to genotype the breeding populations and phenotypic data were analyzed. Nine accessions had a major resistance quantitative trait locus (QTL) within the genomic region containing PdR1. The phenotypic data for these three resistant accessions, ANU67, b41-13, and T03-16, did not associate with PdR1 linked markers, indicating that their resistance is located in other regions of the genome. These three accessions were identified as candidates for use in the development of framework maps with larger populations capable of detecting additional and unique loci for PD resistance breeding and the stacking of PD resistance genes.

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Acknowledgements

Research support from the California Department of Food and Agriculture PD/GWSS Board, and the Louise Rossi Endowed Chair in Viticulture funds is gratefully acknowledged.

Funding

This study was funded by the California Department of Food and Agriculture PD/GWSS Board (Grants nos. 14-0137-SAS and 15-0425-SA).

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Authors and Affiliations

  1. Department of Viticulture and Enology, University of California, Davis, CA, 95616, USA

    S. Riaz, K. Huerta-Acosta, A. C. Tenscher & M. A. Walker

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  1. S. Riaz
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  2. K. Huerta-Acosta
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  3. A. C. Tenscher
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  4. M. A. Walker
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Corresponding author

Correspondence to M. A. Walker.

Additional information

Communicated by Reinhard Toepfer.

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122_2018_3100_MOESM1_ESM.jpg

Supplementary material 1 Average least square means (lsmeans) comparisons of X. fastidiosa (Xf) levels with Tukey’s test among accessions used as references. Results significantly separate the resistant (b43-17, U505-01, and un-inoculated Chardonnay), intermediate (Roucaneuf and Blanc du Bois) and susceptible (inoculated Chardonnay and U505-22) references that were included in each trial. The use of reference accessions allowed comparison of results across different greenhouse and experiment dates (JPEG 85 kb)

122_2018_3100_MOESM2_ESM.jpg

Supplementary material 2 Phenotypic distribution based on the ELISA bacterial titer (X. fastidiosa (Xf) ln colony forming units/ml) in three breeding populations: a F1 population (n = 47) developed from a cross of the susceptible V. vinifera F2-35 and the resistant accession b41-13, b F1 population (n = 27) developed from a cross of V. vinifera F2-35 and resistant parent ANU67, c F1 population (n = 60) developed with resistant parent T03-16 as female and susceptible V. vinifera cultivar Palomino as male. Arrows indicate the resistant (R) and susceptible (S) parent bacterial count. The number of plants within each titer count are shown on top of the bars (JPEG 64 kb)

Supplementary material 3 (DOCX 28 kb)

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Riaz, S., Huerta-Acosta, K., Tenscher, A.C. et al. Genetic characterization of Vitis germplasm collected from the southwestern US and Mexico to expedite Pierce’s disease-resistance breeding. Theor Appl Genet 131, 1589–1602 (2018). https://doi.org/10.1007/s00122-018-3100-z

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