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High-density mapping of the major FHB resistance gene Fhb7 derived from Thinopyrum ponticum and its pyramiding with Fhb1 by marker-assisted selection

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Wheat lines with shortened Th. ponticum chromatin carrying Fhb7 and molecular markers linked to Fhb7 will accelerate the transfer of Fhb7 to breeding lines and provide an important resource for future map-based cloning of this gene.

Abstract

Fusarium head blight is a major wheat disease globally. A major FHB resistance gene, designated as Fhb7, derived from Thinopyrum ponticum, was earlier transferred to common wheat, but was not used in wheat breeding due to linkage drag. The aims of this study were to (1) saturate this FHB resistance gene region; (2) develop and characterize secondary translocation lines with shortened Thinopyrum segments carrying Fhb7 using ph1b; (3) pyramid Fhb7 and Fhb1 by marker-assisted selection. Fhb7 was mapped in a 1.7 cM interval that was flanked by molecular markers XsdauK66 and Xcfa2240 with SSR, diversity arrays technology, EST-derived and conserved markers. KS24-2 carrying Fhb7 was analyzed with molecular markers and genomic in situ hybridization, confirming it was a 7DS.7el2L Robertsonian translocation. To reduce the Thinopyrum chromatin segments carrying Fhb7, a BC1F2 population (Chinese Spring ph1bph1b*2/KS24-2) was developed and genotyped with the markers linked to Fhb7. Two new translocation lines (SDAU1881 and SDAU1886) carrying Fhb7 on shortened alien segments (approximately 16.1 and 17.3 % of the translocation chromosome, respectively) were developed. Furthermore, four wheat lines (SDAU1902, SDAU1903, SDAU1904, and SDAU1906) with the pyramided markers flanking Fhb1 and Fhb7 were developed and the FHB responses indicated lines with mean NDS ranging from 1.3 to 1.6 had successfully combined Fhb7 and Fhb1. Three new molecular markers associated with Fhb7 were identified and validated in 35 common wheat varieties. The translocation lines with shortened alien segments carrying Fhb7 (and Fhb1) and the markers closely linked to Fhb7 will be useful for improving wheat scab resistance.

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Acknowledgments

The authors thank Robert McIntosh, University of Sydney Plant Breeding Institute–Cobbitty, PB4011, Narellan, NSW 2567, Australia, for review of the manuscript before submission. We thank Dr. B. S. Gill, Kansas state university for providing seeds of wheat nulli-tetra and deletion stocks of group 7. We acknowledge financial supports by the National High-Tech R&D Program of China (2011AA100102 and 2012AA101105), the NSF of China (Grant nos. 31171553 and 31471488), Shandong Seed Engineering Project (2015-2019), and the International Collaboration Program (948 Project, 2013-S19).

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Authors and Affiliations

  1. State Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Taian, 271018, China

    Jun Guo, Jinjin Cai, Tingting Zhou, Huihui Xu, Hongwei Wang, Anfei Li, Honggang Wang & Lingrang Kong

  2. College of Life Science, Northeast Forest University, Harbin, 150040, Jilin, China

    Xiuli Zhang

  3. State Key Lab of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China

    Yanlin Hou & Fangpu Han

  4. Department of Agronomy, Purdue University, West Lafayette, IN, 47907-1150, USA

    Xiaorong Shen & Herbert W. Ohm

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  1. Jun Guo
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  2. Xiuli Zhang
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Correspondence to Lingrang Kong.

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Communicated by H. Buerstmayr.

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Guo, J., Zhang, X., Hou, Y. et al. High-density mapping of the major FHB resistance gene Fhb7 derived from Thinopyrum ponticum and its pyramiding with Fhb1 by marker-assisted selection. Theor Appl Genet 128, 2301–2316 (2015). https://doi.org/10.1007/s00122-015-2586-x

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