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Binding of clenbuterol to HSA and FTO: a spectroscopic analysis and molecular docking

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Abstract

Clenbuterol (CLB) is a notorious “lean meat essence”, which has harmful effects on human health. In this study, the binding of CLB to two proteins, human serum albumin (HSA) and the human fat mass and obesity associated protein (FTO), have been investigated by spectroscopic and molecular modeling methods. Fluorescence data revealed that CLB could bind to HSA (or FTO) to form a complex. Thermodynamic analysis and molecular modeling indicated that the binding process was a spontaneous molecular interaction procedure, in which hydrophobic interaction played a major role. Results indicated that CLB could lead to some microenvironmental changes of both proteins.

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Acknowledgements

We are grateful to the National Natural Science Foundation of China (No. 81330075) and 2015 Key science and technology plan project of Henan province (152102310065) for financial support.

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Authors and Affiliations

  1. College of Chemistry and Molecular Engineering, Zhengzhou University, 100 Science Avenue, Zhengzhou, 450001, China

    Zechun Wang, Xinxin Han, Ning Wang, Ruiyong Wang & Junbiao Chang

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  1. Zechun Wang
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  2. Xinxin Han
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  3. Ning Wang
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  4. Ruiyong Wang
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  5. Junbiao Chang
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Correspondence to Ruiyong Wang or Junbiao Chang.

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Wang, Z., Han, X., Wang, N. et al. Binding of clenbuterol to HSA and FTO: a spectroscopic analysis and molecular docking. Med Chem Res 27, 944–953 (2018). https://doi.org/10.1007/s00044-017-2117-1

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  • DOI: https://doi.org/10.1007/s00044-017-2117-1

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