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Isolation, purification and identification of antioxidants from Lepidium latifolium extracts

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Abstract

Antioxidant potencies of an ethanolic extract and its components from Lepidium latifolium were investigated. In this study, we found that the ethyl acetate soluble fraction of L. latifolium was a rich source of antioxidant, resulting from its high total phenolic content. To determine the antioxidant components of the ethyl acetate fraction, a bioassay-guided fractionation approach using 1,1-diphenyl-2-picrylhydrazyl, 2,2′-azinobis-(3-ethylbenzthiazoline-6-sulfonate), and ferric reducing antioxidant power assays were conducted. Nine compounds were isolated and their structures were identified by MS and NMR spectral data and comparison to reported data. They are Quercetin-3-O-β-d-sophoroside-7-O-α-l-rhamnoside (1), Apetalumoside B6 (2), Kaempferol-3-O-β-d-glucopyranosyl-(1-2)-β-d-glucopyranoside-7-O-β-d-glucopyranoside (3), Kaempferol-7-O-α-l-rhamnopyranoside (4), Kaempferol-3-O-β-d-glucopyranoside-7-O-α-l-rhamnopyranoside (5), Kaempferol-3-O-(2-O-feruloyl-β-d-glucopyranosyl-(1-2)-β-d-glucopyranoside)-7-O-glucopyranoside (6), Kaempferol-3-O-β-d-sophoroside-7-O-α-l-rhamnoside (7), Kaempferol-3-O-robinoside-7-O-(2″″-(E)-feruloyl)-sophoroside (8), Quercetin-3-O-(2,6-di-O-β-d-glucopyranosyl)-β-d-glucopyranoside-7-O-α-l-rhamnopyranoside (9), compounds 1, 2, 4, and 8 had potent free radical scavenging activity. The IC50 values of these compounds were 9.8–12.3 and 7.4–31.4 μg/mL in DPPH and ABTS assays, respectively. The results indicate that L. latifolium is a potential natural source of antioxidants to treat several diseases related to oxidant by-products of human metabolism.

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Acknowledgements

This research was sponsored by the Project of Discovery, Evaluation and Transformation of Active Natural Compounds, Strategic Biological Resources Service Network Program of Chinese Academy of Sciences (ZSTH-027).

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Authors and Affiliations

  1. Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, 810001, China

    Yuan Xiang, Wang Haixia, Mei Lijuan & Tao Yanduo

  2. University of Chinese Academy of Sciences, Beijing, 100000, China

    Yuan Xiang

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  1. Yuan Xiang
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  2. Wang Haixia
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  3. Mei Lijuan
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  4. Tao Yanduo
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Correspondence to Tao Yanduo.

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Xiang, Y., Haixia, W., Lijuan, M. et al. Isolation, purification and identification of antioxidants from Lepidium latifolium extracts. Med Chem Res 27, 37–45 (2018). https://doi.org/10.1007/s00044-017-2042-3

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  • DOI: https://doi.org/10.1007/s00044-017-2042-3

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