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GM-CSF and GM-CSF receptor have regulatory role in transforming rat mesenteric mesothelial cells into macrophage-like cells

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Abstract

Objective and design

During peritonitis, mesothelial cells assume macrophage characteristics, expressing macrophage markers, indicating that they might differentiate into macrophage-like cells.

Materials and subjects

Twenty-five male rats were used for in vivo experiments. For in vitro experiments, a primary mesentery culture model was developed. The mesothelial cell to macrophage-like cell transition was followed by studying ED1 expression.

Treatments

In vitro primary mesenteric culture was treated with granulocyte–macrophage colony-stimulating factor (GM-CSF, 1 ng/ml). Blocking internalization of receptor–ligand complex, Dynasore (80 µM) was used. Acute peritonitis was induced by Freund’s adjuvant’s (1 ml) intraperitoneal injection.

Results

Immunohistochemistry: GM-CSF in vitro treatment resulted in a prominent ED1 expression in transformed mesothelial cells. Blocking the internalization, ED1 expression could not be detected. GM-CSF receptor (both α and β) was expressed in mesothelial cells in vitro (even if the GM-CSF was not present) and in vivo. Inflammation resulted in an increasing GM-CSF and GM-CSF-receptor level in the lysate of mesothelial cells.

Conclusions

Mesothelial cells can differentiate into macrophage-like cells, and GM-CSF, produced by the mesothelial cells, has probably an autocrine regulatory role in this transition. Our results provide new data about the plasticity of mesothelial cell and support the idea that during inflammation macrophages can derive from non-hematopoietic sources as well.

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Acknowledgments

We would like to express our thankfulness to Professor Pál Röhlich for the precious comments and accurate language correction of the manuscript. Our special thanks go to Katalin Lőcsey for her valuable technical help.

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Authors and Affiliations

  1. Department of Anatomy, Histology and Embryology, Semmelweis University, Tűzoltó u. 58., Budapest, 1094, Hungary

    Sándor Katz, Viktória Zsiros, Nikolett Dóczi, Arnold Szabó, Ádám Biczó & Anna L. Kiss

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  1. Sándor Katz
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  2. Viktória Zsiros
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  3. Nikolett Dóczi
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  4. Arnold Szabó
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  5. Ádám Biczó
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Correspondence to Anna L. Kiss.

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The authors declare that they have no conflict of interest.

Ethical approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

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Responsible Editor: John Di Battista.

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Katz, S., Zsiros, V., Dóczi, N. et al. GM-CSF and GM-CSF receptor have regulatory role in transforming rat mesenteric mesothelial cells into macrophage-like cells. Inflamm. Res. 65, 827–836 (2016). https://doi.org/10.1007/s00011-016-0967-5

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  • DOI: https://doi.org/10.1007/s00011-016-0967-5

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