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Salmonella enteritidis and Listeria monocytogenes: inactivation effect and aerobic respiratory limitation of cold plasma treatment

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Abstract

Salmonella enteritidis (S. enteritidis) and Listeria monocytogenes (L. monocytogenes) are two main foodborne pathogens. Cold plasma (CP), a non-thermal technology used for food decontamination, has attracted considerable attention in recent years. Hence, we studied the effects of CP treatment on S. enteritidis and L. monocytogenes growth and bacterial respiratory enzyme activities to elucidate the CP disinfection mechanism. Bacterial colonies were counted, respiratory enzyme activity and reactive species (RS) contents were measured, and relationships between these factors were assessed immediately after CP treatment. Both the colony numbers and enzymatic activities of the two bacterial species decreased as the treatment voltage increased from 60 to 80 kV, regardless of the treatment time. Moreover, similar increasing trends in RS were found in treated bacterial samples. The enzymatic activity of S. enteritidis was reduced to a greater extent than that of L. monocytogenes under the same treatment conditions. After CP treatment at 80 kV for 180 s the activity levels of both malate dehydrogenase (MDH) and succinate dehydrogenase (SDH) in S. enteritidis decreased below the detection limit. The RS levels showed a significant negative correlation with respiratory enzyme activities in these bacteria. Cold plasma displayed a high inhibitory effect on respiratory enzymes in both S. enteritidis and L. monocytogenes. SDH and hexokinase showed the highest correlation with the growth of treated S. enteritidis and L. monocytogenes, respectively. These results indicate that CP affects the growth of S. enteritidis and L. monocytogenes through different pathways, with CP disruption of normal aerobic respiration to induce cell death being the main antimicrobial mechanism.

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Acknowledgements

This study was funded by Natural Science Foundation of Hainan province (219QN153) and National Natural Science Foundation of China (31601565).

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  1. College of Food Science and Engineering, Hainan University, Haikou, 570228, China

    Tengfei Fu, Yafu Liu & Jiamei Wang

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  1. Tengfei Fu
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  2. Yafu Liu
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  3. Jiamei Wang
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Correspondence to Jiamei Wang.

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Fu, T., Liu, Y. & Wang, J. Salmonella enteritidis and Listeria monocytogenes: inactivation effect and aerobic respiratory limitation of cold plasma treatment. J Consum Prot Food Saf 17, 243–253 (2022). https://doi.org/10.1007/s00003-022-01376-y

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  • DOI: https://doi.org/10.1007/s00003-022-01376-y

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