Skip to main content

Microalgal Biomass as a Promising Feedstock for the Production of Biohydrogen: A Comprehensive Review

  • Chapter
  • First Online:
Organic Waste to Biohydrogen

Part of the book series: Clean Energy Production Technologies ((CEPT))

Abstract

The global energy crisis and rising greenhouse gas emissions have fueled the search for environmentally acceptable renewable energy sources. Biological hydrogen production from microalgae is a low-cost, clean energy source that generates water vapor as a by-product and does not pollute the environment, unlike fossil fuels, which emit CO2 when burned. Microalgal biomass is a biofuel feedstock that is both environmentally friendly and renewable. These photosynthetic organisms collect carbohydrates naturally, mostly in the form of starch, which is utilized as raw substrates. DNA, RNA (1–5%), and other essential components like antioxidants, vitamins, pigments, and fatty acids are also present in microalgal cells. The majority of the chemical composition of microalgal biomass is made up of proteins, carbs, and lipids. Biophotolysis or photofermentation can be used by microalgae to produce hydrogen. In microalgae metabolism, the enzymes hydrogenase and nitrogenase are principally responsible for the biological hydrogen generation process. Despite successful laboratory-scale research into microalgae hydrogen production, low yield has been identified as a problem due to light absorption efficiency, enzyme sensitivity to oxygen, CO2 fixation efficiency, and other reasons. This review discusses different metabolic pathways of hydrogen production, the enzymes involved, different techniques to recover microalgal biomass, and factors affecting the production of biohydrogen.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 169.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 219.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  • Akhlaghi N, Najafpour-Darzi G (2020) A comprehensive review on biological hydrogen production. Int J Hydrog Energy 45(43):22492–22512

    Article  CAS  Google Scholar 

  • Akkerman I, Janssen M, Rocha J, Wijffels RH (2002) Photobiological hydrogen production: photochemical efficiency and bioreactor design. Int J Hydrog Energy 27:1195–1208

    Article  CAS  Google Scholar 

  • Allahverdiyeva Y, Aro EM, Kosourov SN (2014) Recent developments on cyanobacteria and green algae for biohydrogen photoproduction and its importance in CO2 reduction. In: Gupta V, Tuohy M, Kubicek C, Saddler J, Xu F (eds) Bioenergy research: advances and applications. Elsevier, Amsterdam, pp 367–387

    Chapter  Google Scholar 

  • Amutha KB, Murugesan AG (2011) Biological hydrogen production by the algal biomass Chlorella vulgaris MSU 01 strain isolated from pond sediment. Bioresour Technol 102:194–199

    Article  CAS  Google Scholar 

  • Aubert-Jousset E, Cano M, Guedeney G, Richaud P, Cournac L (2011) Role of HoxE subunit in Synechocystis PCC 6803 hydrogenase. FEBS J 278:4035–4043

    Article  CAS  PubMed  Google Scholar 

  • Azwar MY, Hussain MA, Abdul-Wahab AK (2014) Development of bio-hydrogen production by photo biological: fermentation and electrochemical processes: a review. Renew Sustain Energy Rev 31:158–173

    Article  CAS  Google Scholar 

  • Babel S, Takizawa S (2010) Microfiltration membrane fouling and cake behavior during algal filtration. Desalination 261:46–51

    Article  CAS  Google Scholar 

  • Basak N, Das D (2007) The prospect of purple non-sulfur (PNS) photosynthetic bacteria for hydrogen production: the present state of the art. World J Microbiol Biotechnol 23(1):1e42

    Article  CAS  Google Scholar 

  • Bastidas-Oyanedel J-R, Mohd-Zaki Z, Zeng RJ, Bernet N, Pratt S, Steyer J-P et al (2012) Gas controlled hydrogen fermentation. Bioresour Technol 110:503–509

    Article  CAS  PubMed  Google Scholar 

  • Behera S, Singh R, Arora R, Sharma NK, Shukla M, Kumar S (2015) Scope of algae as third generation biofuels frontiers in bioengineering and biotechnology. Mar Biotechnol 90(2):1–13

    Google Scholar 

  • Bharathiraja B, Chakravarthy M, Ranjith Kumar R, Yogendran D, Yuvaraj D, Jayamuthunagai J (2015) Aquatic biomass (algae) as a future feedstock for biorefineries: a review on cultivation, processing and products. Renew Sustain Energy Rev 47:634e653

    Article  CAS  Google Scholar 

  • Bothe H, Schmitz O, Yates MG, Newton WE (2010) Nitrogen fixation and hydrogen metabolism in cyanobacteria. MicrobiolMolBiol Rev 74:529–551

    CAS  Google Scholar 

  • Brennana L, Owendea P (2010) Biofuels from microalgae—A review of technologies for production, processing, and extractions of biofuels and coproducts. Renew Sustain Energy Rev 14:557–577

    Article  CAS  Google Scholar 

  • Carrieri D, Wawrousek K, Eckert C, Yu J, Maness P-C (2011) The role of the bidirectional hydrogenase in cyanobacteria. Bioresour Technol 102(18):8368–8377

    Article  CAS  PubMed  Google Scholar 

  • Cerff M, Morweiser M, Dillschneider R, Michel A, Menzel K, Posten C (2012) Harvesting fresh water and marine algae by magnetic separation: Screening of separation parameters and high gradient magnetic filtration. Bioresour Technol 118:289–295

    Article  CAS  PubMed  Google Scholar 

  • Chen PC, Fan SH, Chiang CL, Lee CM (2008) Effect of growth conditions on the hydrogen production with cyanobacterium Anabaena sp. strain CH3. Int J Hydrog Energy 33:1460–1464

    Article  CAS  Google Scholar 

  • Chen C-Y, Yeh K-L, Aisyah R, Lee D-J, Chang J-S (2011) Cultivation, photobioreactor design, and harvesting of microalgae for biodiesel production: a critical review. Bioresour Technol 102:71–81

    Article  CAS  PubMed  Google Scholar 

  • Chong M, Sabaratnam V, Shirai Y, Ali M, Hassan MA (2009) Biohydrogen production from biomass and industrial wastes by dark fermentation. Int J Hydrog Energy 34:3277–3287

    Article  CAS  Google Scholar 

  • Chu S, Majumdar A (2012) Opportunities and challenges for a sustainable energy future. Nature 488:294–303

    Article  CAS  PubMed  Google Scholar 

  • Debabrata D, Veziroglu TN (2001) Hydrogen production by biological processes: a survey of literature. Int J Hydrog Energy 26:13–28

    Article  Google Scholar 

  • Degrenne B, Pruvost J, Legrand J (2011) Effect of prolonged hypoxia in autotrophic conditions in the hydrogen production by the green microalga Chlamydomonas reinhardtii in photobioreactor. Bioresour Technol 102:1035–1043

    Article  CAS  PubMed  Google Scholar 

  • Dittami SM, Michel G, Collen J, Boyen C, Tonon T (2010) Chlorophyll-binding proteins revisited - a multigenic family of light-harvesting and stress proteins from a brown algal perspective. BMC Evol Biol 10:365

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • English CM, Eckert C, Brown K, Seibert M, King PW (2009) Recombinant and in vitro expression systems for hydrogenases: New frontiers in basic and applied studies for biological and synthetic H2 production. Dalton Trans 45:9970–9978

    Article  Google Scholar 

  • Eroglu E, Melis A (2016) Microalgal hydrogen production research. Int J Hydrog Energy 41:12772–12798

    Article  CAS  Google Scholar 

  • Ghimire A, Frunzo L, Pirozzi F, Trably E, Escudie R, Lens PNL, Esposito G (2015) A review on dark fermentative biohydrogen production from organic biomass: process parameters and use of by-products. Appl Energy 144:73–95

    Article  CAS  Google Scholar 

  • Ghimire A, Kumar G, Sivagurunathan P, Shobana S, Saratale GD, Kim HW, Luongo V, Esposito G, Munoz R (2017) Bio-hythane production from microalgae biomass: key challenges and potential opportunities for algal bio-refineries. Bioresour Technol 241:525e536

    Article  CAS  Google Scholar 

  • Ghirardi ML (2015) Implementation of photobiological H2 production: the O2 sensitivity of hydrogenases. Photosynth Res 125:383–393

    Article  CAS  PubMed  Google Scholar 

  • Hankamer B, Lehr F, Rupprecht J, Mussgnug J, Posten C, Kruse O (2007) Photosynthetic biomass and H2 production by green algae: from bioengineering to bioreactor scale-up. Physiol Plant 131:10e21

    Article  CAS  Google Scholar 

  • Ho DP, Ngo HH, Guo W (2014) A mini review on renewable sources for biofuel. Bioresour Technol 169:742–749

    Article  CAS  PubMed  Google Scholar 

  • John RP, Anisha GS, Nampoothiri KM, Pandey A (2011) Micro and macroalgal biomass: a renewable source for bioethanol. Bioresour Technol 102:186–193

    Article  CAS  PubMed  Google Scholar 

  • Jung K-W, Kim D-H, Kim S-H, Shin H-S (2011) Bioreactor design for continuous dark fermentative hydrogen production. Bioresour Technol 102:8612–8620

    Article  CAS  PubMed  Google Scholar 

  • Khanna N, Lindblad P (2015) Cyanobacterial hydrogenases and hydrogen metabolism revisited: recent progress and future prospects. Int J Mol Sci 16:10537–10561

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Khetkorn W, Khanna N, Incharoensakdi A, Lindblad P (2014) Metabolic and genetic engineering of cyanobacteria for enhanced hydrogen production. Biofuels 4:535–561

    Article  CAS  Google Scholar 

  • Khetkorn W, Rastogi RP, Incharoensakdi A, Lindblad P, Madamwar D, Pandey A, Larroche C (2017) Microalgal hydrogen production – a review. Bioresour Technol 243:1194–1206

    Article  CAS  PubMed  Google Scholar 

  • Kim DH, Kim MS (2011) Hydrogenases for biological hydrogen production. Bioresour Technol 102(18):8423–8431

    Article  CAS  PubMed  Google Scholar 

  • Kumar G, Shobana S, Chen WH, Bach QV, Kim SH, Atabani AE, Chang JS (2016) A review of thermochemical conversion of microalgal biomass for biofuels: chemistry and processes. Green Chem 19:44–67

    Article  CAS  Google Scholar 

  • Lee K-S, Tseng T-S, Liu Y-W, Hsiao Y-D (2012) Enhancing the performance of dark fermentative hydrogen production using a reduced pressure fermentation strategy. Int J Hydrog Energy 37:15556–15562

    Article  CAS  Google Scholar 

  • Li Y-G, Gao H-S, Li W-L, Xing J-M, Liu H-Z (2009) In situ magnetic separation and immobilization of dibenzothiophene-desulfurizing bacteria. Bioresour Technol 100:5092–5096

    Article  CAS  PubMed  Google Scholar 

  • Liang Y, Sarkanyet N, Cui Y (2009) Biomass and lipid productivities of Chlorella vulgaris under autotrophic, heterotrophic, and mixotrophic growth conditions. Biotechnol Lett 31:1043–1049

    Article  CAS  PubMed  Google Scholar 

  • Lim JK, Chieh DCJ, Jalak SA, Toh PY, Yasin NHM, Ng BW, Ahmad AL (2012) Rapid magnetophoretic separation of microalgae. Small 8:1683–1692

    Article  CAS  PubMed  Google Scholar 

  • Lin CY, Lay CH (2004) A nutrient formulation for fermentative hydrogen production using anaerobic sewage sludge micro flora. Int J Hydrog Energy 30:285–292

    Article  CAS  Google Scholar 

  • Luque R (2010) Algal biofuels: the eternal promise? Energy Environ Sci 3(3):254–257

    Article  CAS  Google Scholar 

  • Mandal B, Nath K, Das D (2006) Improvement of biohydrogen production under decreased partial pressure of H2 by Enterobacter cloacae. Biotechnol Lett 28:831–835

    Article  CAS  PubMed  Google Scholar 

  • Markov SA, Thomas AD, Bazin MJ, Hall DO (1997) Photoproduction of hydrogen by cyanobacteria under partial vacuum in batch culture or in a photobioreactor. Int J Hydrog Energy 22:521

    Article  CAS  Google Scholar 

  • Mata TM, Martins AA, Caetano NS (2010) Microalgae for biodiesel production and other applications: a review. Renew Sustain Energy Rev 14:217–232

    Article  CAS  Google Scholar 

  • Medipally SR, Yusoff FM, Banerjee S, Shariff M (2015) Microalgae as sustainable renewable energy feedstock for biofuel production. Biomed Res Int:1–13

    Google Scholar 

  • Melis A (2000) Green alga hydrogen production: progress, challenges and prospects. Int J Hydrog Energy 27:1217–1228

    Article  Google Scholar 

  • Melis A, Happe T (2001) Hydrogen production. Green algae as a source of energy. Plant Physiol 127:740–748

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Melis A, Zhang LP, Forestier M, Ghirardi ML, Seibert M (2000) Sustained photobiological hydrogen gas production upon reversible inactivation of oxygen evolution in the green alga Chlamydomonas reinhardtii. Plant Physiol 122:127–135

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Meyer J (2007) [FeFe] hydrogenases and their evolution: a genomic perspective. Cell Mol Life Sci 64:1063–1084

    Article  CAS  PubMed  Google Scholar 

  • Misra N, Panda PK, Parida BK, Mishra BK (2016) Way forward to achieve sustainable and cost-effective biofuel production from microalgae: a review. Int J Environ SciTechnol 13(11):2735–2756

    Google Scholar 

  • Mutanda T, Ramesh D, Karthikeyan S, Kumari S, Anandraj A, Bux F (2011) Bioprospecting for hyperelipid producing microalgal strains for sustainable biofuel production. Bioresour Technol 102:57–70

    Article  CAS  PubMed  Google Scholar 

  • Nagarajan D, Lee D-J, Kondo A, Chang J-S (2017) Recent insights into biohydrogen production by microalgae – from biophotolysis to dark fermentation. Bioresour Technol 227:373–387

    Article  CAS  Google Scholar 

  • Nagarajan D, Chang J-S, Lee D-J (2020a) Pretreatment of microalgal biomass for efficient biohydrogen production – recent insights and future perspectives. Bioresour Technol 302:122871

    Article  CAS  PubMed  Google Scholar 

  • Nagarajan D, Dong C, Chen C, Lee D, Chang J (2020b) Biohydrogen production from microalgae – major bottlenecks and future research perspectives. Biotechnol J 16(5):2000124

    Article  CAS  Google Scholar 

  • Neves VTDC, Andrade E, Perelo LW (2016) Influence of lipid extraction methods as pre-treatment of microalgal biomass for biogas production. Renew Sustain Energy Rev 59:160–165

    Article  CAS  Google Scholar 

  • Nyberg M, Heidorn T, Lindblad P (2015) Hydrogen production by the engineered cyanobacterial strain NostocPCC 7120 D hupW examined in a flat panel photo bioreactor system. J Biotechnol 215:35–43

    Article  CAS  PubMed  Google Scholar 

  • Obradovic A, Likozar B, Levec J (2013) Catalytic surface development of novel nickel plate catalyst with combined thermally annealed platinum and alumina coatings for steam methane reforming. Int J Hydrog Energy 38:1419–1429

    Article  CAS  Google Scholar 

  • Oey M, Sawyer AL, Ross IL, Hankamer B (2016) Challenges and opportunities for hydrogen production from microalgae. Plant Biotechnol J 14:1487–1499

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Oh H-M, Lee SJ, Park M-H, Kim H-S, Kim H-C, Yoon J-H et al (2001) Harvesting of Chlorella vulgaris using a bioflocculant from Paenibacillus sp. AM49. Biotechnol Lett 23:1229–1234

    Article  CAS  Google Scholar 

  • Papazi A, Gjindali A-I, Kastanaki E, Assimakopoulos K, Stamatakis K, Kotzabasis K (2014) Potassium deficiency, a “smart” cellular switch for sustained high yield hydrogen production by the green alga Scenedesmus obliquus. Int J Hydrog Energy 39:19452–19464

    Article  CAS  Google Scholar 

  • Prochazkova G, Safarik I, Branyik T (2013) Harvesting microalgae with microwave synthesized magnetic microparticles. Bioresour Technol 130:472–477

    Article  CAS  PubMed  Google Scholar 

  • Pushparaj B, Pelosi E, Torzillo G, Materassi R (1993) Microbial biomass recovery using a synthetic cationic polymer. Bioresour Technol 43:59–62

    Article  CAS  Google Scholar 

  • Ramachandran R, Menon RK (1998) An overview of industrial uses of hydrogen. Int J Hydrog Energy 23:593–598

    Article  CAS  Google Scholar 

  • Rashid N, Rehman MS, Memonb S, Rahman Z, Lee K, Han JI (2013) Current status, barriers and developments in bio-hydrogen production by microalgae. Renew Sustain Energy Rev 22:571–579

    Article  CAS  Google Scholar 

  • Rathore D, Singh A (2013) Biohydrogen production from microalgae. In: Gupta VK, Tuohy MG (eds) Biofuel technologies - recent developments. Springer, Berlin, pp 317–333

    Chapter  Google Scholar 

  • Razu MH, Hossain F, Khan M (2019) Advancement of bio-hydrogen production from microalgae. In: Microalgae biotechnology for development of biofuel and wastewater treatment. Springer, Singapore, pp 423–462

    Chapter  Google Scholar 

  • Ríos SD, Salvadó J, Farriol X, Torras C (2012) Antifouling microfiltration strategies to harvest microalgae for biofuel. Bioresour Technol 119:406–418

    Article  PubMed  CAS  Google Scholar 

  • Sambusiti C, Bellucci M, Zabaniotou A, Beneduce L, Monlau F (2015) Algae as promising feedstocks for fermentative biohydrogen production according to a biorefinery approach: a comprehensive review. Renew Sustain Energy Rev 44:20e36

    Article  CAS  Google Scholar 

  • Scoma A, Krawietz D, Faraloni C, Giannelli L, Happe T, Torzillo G (2012) Sustained H2 production in a Chlamydomonasreinhardtii D1 protein mutant. J Biotechnol 157:613–619

    Article  CAS  PubMed  Google Scholar 

  • Shobana S, Saratale GD, Pugazhendhi A, Arvindnarayan S, Periyasamy S, Kumar G, Kim SH (2017) Fermentative hydrogen production from mixed and pure microalgae biomass: key challenges and possible opportunities. Int J Hydrog Energy 42:26440–26453

    Article  CAS  Google Scholar 

  • Show K-Y, Lee D-J, Chang J-S (2013) Algal biomass dehydration. Bioresour Technol 135:720–729

    Article  CAS  PubMed  Google Scholar 

  • Show K-Y, Yan Y-G, Lee D-J (2019) Biohydrogen production from algae: perspectives, challenges, and prospects. Biofuels from algae 325–343

    Google Scholar 

  • Singh NK, Sonani RR, Rastogi RP, Madamwar D (2015) The phycobilisomes: An early requisite for efficient photosynthesis in cyanobacteria. EXCLI J 14:268–289

    PubMed  PubMed Central  Google Scholar 

  • Srirangan K, Pyne ME, Perry CC (2011) Biochemical and genetic engineering strategies to enhance hydrogen production in photosynthetic algae and cyanobacteria. Bioresour Technol 102:8589–8604

    Article  CAS  PubMed  Google Scholar 

  • Tamagnini P, Leitao E, Oliveira P, Ferreira D, Pinto F, Harris DJ, Heidorn T, Lindblad P (2007) Cyanobacterial hydrogenases: diversity, regulation and applications. FEMS Microbiol Rev 31:692–720

    Article  CAS  PubMed  Google Scholar 

  • Toh PY, Yeap SP, Kong LP, Ng BW, Derek CJC, Ahmad AL, Lim J (2012) Magnetophoretic removal of microalgae from fishpond water: feasibility of high gradient and low gradient magnetic separation. Chem Eng J 211:22–30

    Article  CAS  Google Scholar 

  • Troshina O, Serebryakova L, Sheremetieva M, Lindblad P (2002) Production of H2 by the unicellular Cyanobacterium gloeocapsa alpicola CALU743 during fermentation. Int J Hydrog Energy 27:1283

    Article  CAS  Google Scholar 

  • Uduman N, Qi Y, Danquah MK, Forde GM, Hoadley A (2010) Dewatering of microalgal cultures: a major bottleneck to algae-based fuels. J Renew Sustain Energy 2:012701

    Article  CAS  Google Scholar 

  • Uggetti E, Sialve B, Trably E, Steyer JP (2014) Integrating microalgae production with anaerobic digestion: a biorefinery approach. Biofuels Bioprod Biorefin 8(4):516–529

    Article  CAS  Google Scholar 

  • Vogt S, Lyon EJ, Shima S, Thauer RK (2008) The exchange activities of [Fe] hydrogenase (iron-sulfur-cluster-free hydrogenase) from methanogenic archaea in comparison with the exchange activities of [FeFe] and [NiFe] hydrogenases. J Biol Inorg Chem 13:97–106

    Article  CAS  PubMed  Google Scholar 

  • Volgusheva A, Styring S, Mamedov F (2013) Increased photosystem II stability promotes H2 production in sulfur-deprived Chlamydomonas reinhardtii. Proc Natl Acad Sci U S A 110:7223–7228

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Wang J, Wan W (2009) Factors influencing fermentative hydrogen production: A review. Int J Hydrog Energy 34:799–811

    Article  CAS  Google Scholar 

  • Wang JL, Yin YN (2017) Biohydrogen production from organic wastes. Springer, Singapore

    Book  Google Scholar 

  • Wang Y, Wang H, Feng X, Wang X, Huang J (2010) Biohydrogen production from cornstalk wastes by anaerobic fermentation with activated sludge. Int J Hydrog Energy 35:3092e9

    Google Scholar 

  • Winkler M, Kuhlgert S, Hippler M, Happe T (2009) Characterization of the key step for light-driven hydrogen evolution in green algae. J Biol Chem 284:36620–36627

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Wykoff DD, Davies JP, Melis A, Grossman AR (1998) The regulation of photosynthetic electron-transport during nutrient deprivation in Chlamydomonas reinhardtii. Plant Physiol 117:129–139

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Xu L, Guo C, Wang F, Zheng S, Liu C-Z (2011) A simple and rapid harvesting method for microalgae by in situ magnetic separation. Bioresour Technol 102:10047–10051

    Article  CAS  PubMed  Google Scholar 

  • Yang Z, Guo R, Xu X, Fan X, Li X (2010) Enhanced hydrogen production from lipid-extracted microalgal biomass residues through pretreatment. Int J Hydrog Energy 35(18):9618–9623

    Article  CAS  Google Scholar 

  • Yen HW, Hu IC, Chen CY, Ho SH, Lee DJ, Chang JS (2013) Microalgae-based biorefinery—from biofuels to natural products. Bioresour Technol 135:166–174

    Article  CAS  PubMed  Google Scholar 

  • Yilmaz F, Balta MT, Selbas R (2016) A review of solar based hydrogen production methods. Renew Sustain Energ Rev 56:171–178

    Article  CAS  Google Scholar 

  • Yin YN, Wang JL (2015) Biohydrogen production using waste activated sludge disintegrated by gamma irradiation. Appl Energy 155:434–439

    Article  CAS  Google Scholar 

  • Zhan J, Zhang Q, Qin M, Hong Y (2016) Selection and characterization of eight freshwater green algae strains for synchronous water purification and lipid production. Front Environ Sci Eng 10(3):548–558

    Article  CAS  Google Scholar 

  • Zhang L, Happe T, Melis A (2000) Biochemical and morphological characterization of sulfur-deprived and H2-producing Chlamydomonas reinhardtii (green alga). Planta 214:552–561

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Esha Rami .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2022 The Author(s), under exclusive license to Springer Nature Singapore Pte Ltd.

About this chapter

Check for updates. Verify currency and authenticity via CrossMark

Cite this chapter

Singh, A., Das, R., Upadhye, V., Rami, E. (2022). Microalgal Biomass as a Promising Feedstock for the Production of Biohydrogen: A Comprehensive Review. In: Kuddus, M., Yunus, G., Ramteke, P.W., Molina, G. (eds) Organic Waste to Biohydrogen. Clean Energy Production Technologies. Springer, Singapore. https://doi.org/10.1007/978-981-19-1995-4_11

Download citation

Publish with us

Policies and ethics