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Microfluidics in Chemical Biology

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Microfluidics and Multi Organs on Chip

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Abstract

Chemical biology is the field where two scientific disciplines, that is, chemistry and biology, join. Techniques derived from chemistry are used to study or manipulate biological or natural products. Chemical biology helps develop bioassays for the quantification of various compounds. The wisdom from chemistry is used to develop techniques to purify proteins and nucleic acids or use small molecules for multiple applications, such as sensing, drug discovery, tissue engineering, disease modelling and molecular genetics. The 1970s witnessed the development of a novel field of engineering that manipulates micro- or nano-quantities of fluids using channels and pumps for specific applications called microfluidics. The development of various microfabrication techniques and novel materials in the past few decades has led to efficient microfluidic platforms. The convergence of microfluidics, chemistry and biology gives us a platform where minute quantities of samples are orchestrated to ensemble an overall result that helps researchers in various scientific fields. This chapter briefly discusses the application of microfluidics in chemical biology in bioassays, separation and purification of proteins and nucleotides, molecular self-assembly, tissue engineering and nucleotide sequencing. These aspects include exposure to electrophoresis and chromatography in microfluidic devices, gradients in microfluidic devices, surface modification strategies, and polymerase chain reaction (PCR).

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  1. School of Biotechnology, National Institute of Technology Calicut, Kozhikode, Kerala, India

    Abey Joseph, Akash Karthikeyan, Shamili G. Nair, V. R. Prince, P. E. Nafrin & Baiju G. Nair

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  1. Toxicology Division, Biomedical Technical Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology (Government of India), Thiruvananthapuram, Kerala, India

    P. V. Mohanan

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Joseph, A., Karthikeyan, A., Nair, S.G., Prince, V.R., Nafrin, P.E., Nair, B.G. (2022). Microfluidics in Chemical Biology. In: Mohanan, P.V. (eds) Microfluidics and Multi Organs on Chip . Springer, Singapore. https://doi.org/10.1007/978-981-19-1379-2_4

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