Abstract
Biohydrogen is a clean fuel, which can be produced through direct or indirect biophotolysis, photo-fermentation, and dark fermentation by microorganisms. In dark fermentation, organic waste degradation and hydrogen production go simultaneously. A variety of substrates from industrial wastewater to agricultural solid wastes have been used for biohydrogen production. Obligate anaerobes from genera Clostridium and Desulfovibrio species and facultative anaerobes from Citrobacter, Enterobacter, Klebsiella, and Bacillus species are known to utilize the organic wastes to produce hydrogen through dark fermentation. Biohydrogen production in batch and fed-batch reactors at lab-scale to pilot scale have been demonstrated by several researchers. The major bottlenecks for the large-scale production of biohydrogen are the costs of plant establishment and maintenance. This study gives an overview of the potential microbes and technology involved in the biohydrogen production from organic wastes through dark fermentation and the factors to be addressed for its commercial production.
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Jamaldheen, S.B., Moholkar, V.S., Goyal, A. (2022). Commercial Production of Biohydrogen Using Microbes. In: Nayak, S.K., Baliyarsingh, B., Mannazzu, I., Singh, A., Mishra, B.B. (eds) Advances in Agricultural and Industrial Microbiology. Springer, Singapore. https://doi.org/10.1007/978-981-16-8918-5_9
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