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Enhanced Growth and Overcoming Abnormal Phenomena in Micropropagation by Nanoparticles

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Plant Tissue Culture: New Techniques and Application in Horticultural Species of Tropical Region

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Abstract

This chapter provides background technical information and demonstrates some positive effects of different metal nanoparticles on the growth and development of in vitro cultured plantlets. Metal nanoparticles were obtained by the aqueous solution method with the core composing of silver, iron, or cobalt (Ago, Feo, Coo). The shell of silver nanoparticles was made of chitosan, and the shell component for iron and cobalt nanoparticles was carboxymethyl cellulose. The abscission phenomenon in Rose plantlets (Rosa hybrida L. “Baby Love”) was suppressed when cobalt nanoparticles (CoNPs) were incorporated in the medium. During the micropropagation of Gerbera (Gerbera jamesonii “Revolution yellow”), silver nanoparticles (AgNPs) promoted plantlet growth and overcame the vitrification phenomenon. Iron nanoparticles (FeNPs) improved the quality of carnation (Dianthus caryophyllus “Express golem”) plantlets, increasing rooting efficiency and tolerance to culture conditions through increased activity of antioxidant enzymes. AgNPs and CoNPs helped to limit ethylene gas biosynthesis and action. The plantlets derived from in vitro cultures supplemented with AgNPs, CoNPs, and FeNPs gave the highest survival percentage and improved development at the nursery stage compared to the control without metal nanoparticle treatments.

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Authors and Affiliations

  1. Tay Nguyen Institute for Scientific Research, VAST, Dalat City, Vietnam

    Duong Tan Nhut, Ha Thi My Ngan, Nguyen Thi Nhu Mai, Phan Le Ha Nguyen & Hoang Thanh Tung

  2. University of Science, VNU—HCMC, Hochiminh City, Vietnam

    Bui Van Le

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  1. Duong Tan Nhut
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Editors and Affiliations

  1. Molecular Biology and Plant Breeding, Tay Nguyen Institute for Scientific Research, Vietnam Academy of Science and Technology, Dalat City, Vietnam

    Duong Tan Nhut

  2. Molecular Biology and Plant Breeding, Tay Nguyen Institute for Scientific Research, Vietnam Academy of Science and Technology, Dalat City, Vietnam

    Hoang Thanh Tung

  3. Biological Sciences, University of Calgary, Calgary, AB, Canada

    Edward Chee-Tak YEUNG

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Nhut, D.T., Ngan, H.T.M., Mai, N.T.N., Nguyen, P.L.H., Van Le, B., Tung, H.T. (2022). Enhanced Growth and Overcoming Abnormal Phenomena in Micropropagation by Nanoparticles. In: Nhut, D.T., Tung, H.T., YEUNG, E.CT. (eds) Plant Tissue Culture: New Techniques and Application in Horticultural Species of Tropical Region. Springer, Singapore. https://doi.org/10.1007/978-981-16-6498-4_15

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