Abstract
Zebrafish is one of the emerging animal models for drug screening. A neuronal system similar to humans makes zebrafish a potential candidate as an animal model for neurotoxicity and neurogenic diseases. Additionally, due to its transparent larvae, it provides mechanistic insights of drug actions in real time. Behavioural, proteomics and metabolomics studies of zebrafish help to understand the various changes that occur in the brain function and pathways due to the neurotoxic effects. However, to successfully carry out the experiments, efficient, easy and cost-effective protocols should be followed. This chapter provides detailed protocols of experiments for a comprehensive neurotoxicity study using the zebrafish model. Zebrafish behaviour is robust. The behavioural study is a non-invasive and quick method to assess neurotoxicity. Novel tank test, colour preference test, social behaviour and cognitive behaviour analysis of zebrafish are well documented in the literature, and numerous researches have been reported. The recent development of omics techniques such as metabolomics and proteomics, along with bioinformatics, provides an excellent opportunity to study alteration of protein expression and neurochemicals due to induction of any neurotoxic drug. The focus of this chapter is the systematic designing of experiments for neurotoxic study.
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Biswas, S., Bellare, J. (2022). Zebrafish Model for Neurotoxic Drug Screening: Methodologies and Protocols. In: Bhandari, P.R., Bharani, K.K., Khurana, A. (eds) Zebrafish Model for Biomedical Research . Springer, Singapore. https://doi.org/10.1007/978-981-16-5217-2_21
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