Abstract
The quantification of interaction between electromagnetic radiation and matter serves as an effective tool in the characterization of materials in order to identify and quantify specific substances. Absorption spectroscopy is based on the phenomenon of wavelength-dependent absorption, that is, the attenuation of radiation intensity when passed through solution containing sample in turn helps to quantify the concentration as well as the nature of substances present within the sample.
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References
Ball DW (2001) The basics of spectroscopy, vol 49. Spie Press, Bellingham
Baruah M et al (2006) Solvent and pH-dependent fluorescent properties of a dimethylaminostyryl borondipyrromethene dye in solution. Chem A Eur J 110(18):5998–6009
Kirby EP, Steiner RF (1970) Influence of solvent and temperature upon the fluorescence of indole derivatives. J Phys Chem 74(26):4480–4490
Bravaya KB et al (2010) Electronic structure and spectroscopy of nucleic acid bases: ionization energies, ionization-induced structural changes, and photoelectron spectra. Chem A Eur J 114(46):12305–12317
Ullrich S et al (2004) Electronic relaxation dynamics in DNA and RNA bases studied by time-resolved photoelectron spectroscopy. Phys Chem Chem Phys 6(10):2796–2801
Šponer J, Leszczynski J, Hobza P (2001) Electronic properties, hydrogen bonding, stacking, and cation binding of DNA and RNA bases. Biopolymers 61(1):3–31
Hannon MJ (2007) Supramolecular DNA recognition. Chem Soc Rev 36(2):280–295
Mandel M, Marmur J (1968) [109] Use of ultraviolet absorbance-temperature profile for determining the guanine plus cytosine content of DNA. In: Methods in enzymology. Elsevier, Amsterdam, pp 195–206
Mach H et al (1995) Ultraviolet absorption spectroscopy. In: Protein stability and folding. Springer, pp 91–114
Wetlaufer D (1963) Ultraviolet spectra of proteins and amino acids. In: Advances in protein chemistry. Elsevier, pp 303–390
Levine RL, Federici MM (1982) Quantitation of aromatic residues in proteins: model compounds for second-derivative spectroscopy. Biochemistry 21(11):2600–2606
Lord R, Gubensek F, Rupley J (1973) Insulin self-association. Spectrum changes and thermodynamics. Biochemistry 12(22):4385–4392
Porterfield JZ, Zlotnick A (2010) A simple and general method for determining the protein and nucleic acid content of viruses by UV absorbance. Virology 407(2):281–288
Mihashi K, Ooi T (1965) Location of abnormal tyrosines in actin. Biochemistry 4(5):805–813
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Basu, M., Mishra, P.P. (2022). Absorption Spectroscopy: What Can We Learn About Conformational Changes of Biomolecules?. In: Sahoo, H. (eds) Optical Spectroscopic and Microscopic Techniques. Springer, Singapore. https://doi.org/10.1007/978-981-16-4550-1_1
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DOI: https://doi.org/10.1007/978-981-16-4550-1_1
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