Skip to main content

Present and Future Prospectives of Microbial Fibrinolytic Enzyme Production and Its Applications

  • Conference paper
  • First Online:
Proceedings of the 2nd International Conference on Computational and Bio Engineering

Part of the book series: Lecture Notes in Networks and Systems ((LNNS,volume 215))

  • 381 Accesses

Abstract

Cardiovascular diseases (CVDs) are the group of diseases that occur in blood vessels and heart, the most common cause of death worldwide. The treatment of this cardiovascular disease is using as an enzyme treatment such as streptokinase, urokinase, tPA, etc. This type of enzymatic drugs has some side effects. To overcome this problem, novel therapeutic enzymes are isolated from different sources. This review focused on novel microorganisms fibrinolytic enzymes produced from different sources and different types of thrombosis. This review is also focused on fibrinolytic enzymes or thrombolytic agents act on novel coronavirus.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 189.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 249.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Lu F, Lu Z, Bie X et al (2010) Purification and characterization of a novel anticoagulant and fibrinolytic enzyme produced by endophytic bacterium Paenibacillus polymyxa EJS-3. Thromb Res 126:349–355

    Article  Google Scholar 

  2. Mahajan PM, Gokhale SV, Lele SS (2010) Production of nattokinase using B. natto NRRL 3666: media optimization, scale up and kinetic modeling. Food Sci Biotechnol 19:1593–1603

    Article  Google Scholar 

  3. Mahajan PM, Nayak S, Lele SS (2012) Fibrinolytic enzyme from newly isolated marine bacterium Bacillus subtilis ICTF-1: media optimization, purification and characterization. J Biosci Bioeng 113(3):307–314

    Article  Google Scholar 

  4. Liu X, Zheng X, Qian P et al (2014) Purification and characterization of a novel fibrinolytic enzyme from culture supernatant of Pleurotus ostreatus. J Microbiol Biotechnol 24(2):245–253

    Article  Google Scholar 

  5. Mohammed AS, Ling TC, Muniandy S, Tan YS, Raman J, Sabaratanam V (2014) Recovery and partial purification of Fibrinolytic enzymes of Auricular polytricha (mont) sall by an aqueous two-phase system. Sep Purif Technol 122i:359–366

    Google Scholar 

  6. Mienda BS, Yahya A, Galadima IA, Shamsher MS (2014) An overview of microbial proteases for industrial application. Res J pharm Biol. Chem. Sci 5:388–396

    Google Scholar 

  7. Kotb E (2015) the biotechnological potential of subtilisin-like fibrinolytic enzyme from a newly isolated Lactobacillus plantarum KSK-II in blood distaining and antimicrobials. Biotechnol Progr 31(2):316–324

    Article  Google Scholar 

  8. Kotb E (2012) Fibrinolytic bacterial enzymes with thrombolytic activity. Springer, New York

    Book  Google Scholar 

  9. Pammi N, Chaitanaya KI, Mahmood SK (2015) Servattia marcseens OUSOT. SP Nov1. A cellulose and pharma. Producing bacterium isolated from polluted water. Int J Environ Biol 5:32–36

    Google Scholar 

  10. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA 6: Molecular Evolutionary Genetics analysis version 6. Mol Biol Evol 36:2725–2729

    Google Scholar 

  11. Kotb E (2013) Activity assessment of microbial fibrinolytic enzymes. Appl Microbiol Biotechnol 97:6647–6665

    Article  Google Scholar 

  12. Kotb E (2014) the biotechnological potential of fibrinolytic enzymes in the dissolution of endogenous blood thrombi. Biotechnol Progr 30(3):656–672

    Article  Google Scholar 

  13. E silva FD, de Azevedo CA (2016) The Assistant software version 7-7 its isle in the analysis of experimental data. Afr J Agric Res 11:3733–3740

    Google Scholar 

  14. Wu R, Chen G, Pan S (2019) Cost-effective fibrinolytic enzyme production by Bacillus subtilis WR 350 using medium supplemented with corn steep powder and sucrose

    Google Scholar 

  15. Kotb E (2014) Purification and partial characterization of a chymotrypsin-like serine fibrinolytic enzyme from Bacillus amyloliquefaciens FCF-11 using corn husk as a novel substrate. World J Microbiol Biotechnol 30(7):2071–2080

    Article  Google Scholar 

  16. Chung DM, Choi NS, Maeng PJ, Chun HK, Kim SH (2010) Purification and characterization of a novel fibrinolytic enzyme from Chive (Allium tuberosum). Food Sci Biotechnol 19:697–702

    Article  Google Scholar 

  17. Lu X, Kopparapu NK, Yao L, Deng Y, Zheng X (2017) Bio-chemical characterization of a novel fibrinolytic enzyme from Cordyceps militaris. Int J Biol Macromol 94:793–801

    Google Scholar 

  18. Vijayaraghavan P et al (2017) Novel sequential sequential screening and enhanced production of fibrinolytic enzyme by Bacillus SP. IND12 using response surface methodology in solid state fermentation. Biomed Res

    Google Scholar 

  19. Kotb E (2015) Purification and partial characterization of serine fibrinolytic enzyme from Bacillus megaterium KSK-07 isolated from Kishk, a traditional Egyptian fermented food. Appl Biochem Microbiol 51(1):34–43

    Article  Google Scholar 

  20. Kotb E (2017) Microbial fibrinolytic enzyme production and applications. In: Microbial Functional Foods and Nutraceuticals, pp 175–213

    Google Scholar 

  21. Yogeh D, Halami PM (2017) Fibrinolytic enzymes of Bacillus SPP. an overview. Int Food J 24(1):35–47

    Google Scholar 

  22. Almalki MA (2018) Solid state fermentation of agro-residues for the production of analyse from Bacillus subtitlis for industrial application. Int J Curr Microbiol Appl Sci 7(3):1341–1348

    Google Scholar 

  23. Green MR, Sambrook J (2012) Molecular cloning a laboratory manual. Cold Spring Harbor Laboratory Press, New York

    Google Scholar 

  24. World Health Organization (2019) The world health report. Cardiovascular diseases (CVD’s)

    Google Scholar 

  25. Ningthoujam DS, Thokchom S (2016) Screening of fibrinolytic enzyme from microorganisms especially actinomycites from different biotypes in Manipur 7(3):21

    Google Scholar 

  26. Thokchom S,. Joshi SR (2014) Screening of fibrinolytic enzymes from lactic acid bacterial isolates associated with traditional fermented soybean foods. Food Sci Biotechnol 23:1601–1604

    Google Scholar 

  27. VijayaRaghavan P, Prakash Vincent SG (2014) Medium optimization for the production of fibrinolytic enzyme by paenibacillus sp., IND8 using response surface methodology scientific world 2014:276942

    Google Scholar 

  28. Krishnamoorthy A, Belur PD, Subramanaya SB (2018) Methods available to assess therapeutic potential of fibrinolytic enzymes of microbial origin a review 9:10

    Google Scholar 

  29. Cui H, Yang M, Wang L, Xian CJ (2015) Identification of new marine bacterial strain SD 8 and optimization of its culture conditions for producing alkaline protease. PLOS One 10:eo14606

    Google Scholar 

  30. Sakpal HC, Narayan G (2015) Thermostable alkaine protease from Bacillus Sp., and its potential application. J Pharm Biol Sci 10:58–67

    Google Scholar 

  31. Vuillemin M, Malbert Y, Laguerre S, Remaud-simeon M, Moulis C(2014) Optimizing the production of an α-(1-22) branching. Sucrose in Escherichia coli using statistical design appl. Microbiol Biotechnol 98:5173–5184

    Google Scholar 

  32. Chandrasekharan SD, Vaithilingam MS, Shankar R (2015) Exploring invitro thromblytic activity of natto kings from a new strain pseudomonas aeruginosa CMSS 8(10). https://doi.org/10.5812/jjm.23567

  33. El Hidri D, Guesmi A, Najjari A, Cherif H, Ettomuni B, Hamdi C et al (2013) Cultivation-dependent assessment, diversity, and ecology of halo alkhalophilic bacteria in arid saline systems of southern Tunisia. Biomed Res Int 648141

    Google Scholar 

  34. Devi CS, Sinha D, Sharma V, Mohana Srinivasan V (2012) Screening for staphylokinase producing staphylococcus spp. from different environmental sample. Asian J Pharm Clin Res 5(4):125–128

    Google Scholar 

  35. https://www.healthline.com/health/coronavirus-and-blood-clots

  36. Narshiman MK, Chandrasekharan M, Rajesh M (2015) Fibrinolytic enzyme, production by newly isolated blood clot lysis potential. Appl Microbiol 61:157–164

    Google Scholar 

  37. Pathak AP, Deshmukh KB (2012) Alkaline protease production, extraction and characterization from alkaliphilic Bacillus licheniformis KBDL4: a lonar soda lake isolate. Indian J Exp Biol 50:569–576

    Google Scholar 

  38. Liu Z, Zheng W, Ge C, Cui W (2019) High-level extracellular production of recombinant nattokinase in Bacillus subtilis WB 800 by multiple tandem promoters

    Google Scholar 

  39. Jo HD, Lee HA, Jeong SJ, Kim JH (2011) Purification and characterization of a major fibrinolytic enzyme from Bacillus amyloliquefaciens MJ 5-41 isolated from Meju. J Microbiol Biotechnol 21(11):1166–1167

    Google Scholar 

  40. Afifah DN, Scikhan M, Syah D, Yanti, Suhartono MT, Kim JH (2014) Purification and characterization of a fibrinolytic enzyme from bacillus pumilus 2-g. Isolated from Gembus an Indonesian fermented food. Prev Nutr Food Sci 19:213–219

    Google Scholar 

  41. Mohanasrinivasan V, Subatra Devi C, Biswas R, Paul F, Mitra M, Selvarajan E, Suganthi V (2013) Enhanced production of natto kinase from UV mutated Bacillus S.P. Bangladesh J Pharmacol 8:110–115

    Article  Google Scholar 

  42. Chateterjee S (2015) Production and estimation alkaline protease by immobilized Bacillus licheniformis isolated from poultry farm SOTL of 24 parganas and its reusability. Adv Pharm Technol Res 6:2–6

    Google Scholar 

  43. Sivanandhini T, Subbaiya R, Gopinath M, Mahavined Angrash JKV, Kabilah T, Selvam M (2015) An investigation on morphological characterization of actionmycetes isolated from marine sediments Res J Pharm Biol Chem Sci 6i:1234–1243

    Google Scholar 

  44. Kim DW, Choi JH, Park SE, Kim S, K S et al (2015) Purification and characterization of a fibrin lytic enzyme from Petasites japhonicas. Int J Biol Macromol 72:1159–1167

    Article  Google Scholar 

  45. Rhasmi B, Liny P (2013) Production and characterization of novel fibrinolytic enzyme from different soil fungal SP. Int J Pharma Bio Sci 43:454–464

    Google Scholar 

  46. Phanksri T, Luxanani P, Panyim S, Tirasophon W (2015) Synergism of regulatory elements in sigma (B) and sigma (A)—dependent promoters enhances recombinant protein expression in Bacillus subtilis. J Bio Eng 20(4):470–475

    Google Scholar 

  47. Xin X, Ambati RR, Cal ZW, Lei B (2019) Development of universal purification protocols for fibrinolytic enzyme – producing bacilli. CYTA J Food 17(1):112–120. ISSN: 1947-6337

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to R. Jaya Madhuri .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2021 The Author(s), under exclusive license to Springer Nature Singapore Pte Ltd.

About this paper

Check for updates. Verify currency and authenticity via CrossMark

Cite this paper

Gowthami, K., Jaya Madhuri, R. (2021). Present and Future Prospectives of Microbial Fibrinolytic Enzyme Production and Its Applications. In: Jyothi, S., Mamatha, D.M., Zhang, YD., Raju, K.S. (eds) Proceedings of the 2nd International Conference on Computational and Bio Engineering . Lecture Notes in Networks and Systems, vol 215. Springer, Singapore. https://doi.org/10.1007/978-981-16-1941-0_22

Download citation

Publish with us

Policies and ethics