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RNA- and DNA-Based Identification of Body Fluids

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Forensic DNA Typing: Principles, Applications and Advancements

Abstract

The most common form of evidence to forensic investigators is the body fluids collected at crime scenes. They ascertain the suspect or victim, they provide valuable DNA evidences and can play a vital role in acquit an innocent individual. The determination of a specific bodily fluid is predominantly the initial step as the body fluid composition is very relevant to the further investigation process. The ability to identify and report an unexplained stain at the scene of crime without waiting for the laboratory results is another very important phase in the forensic body fluids analysis. Many forms of detection methods for body fluids have been known for over a century, such as alternative light source, immunological tests, spectroscopic techniques, chemical methods, catalytic tests, and microscopic methods. Although these modern forms of detection of body fluids are often definitive, these are done at a time with only one body fluid. Currently the usage of molecular genetic based approaches using DNA methylation detection or RNA-based profiling methods has recently conquered to replace the traditional body fluids identification methods.

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Authors and Affiliations

  1. Department of Forensic Science, Guru Ghasidas Vishwavidyalaya, Bilaspur, CG, India

    Arjun Rao, Manisha Rana, Ashish Pradhan & Moumita Sinha

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  1. Arjun Rao
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  2. Manisha Rana
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  3. Ashish Pradhan
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  4. Moumita Sinha
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Editors and Affiliations

  1. DNA Fingerprinting Unit, Department of Home (Police), State Forensic Science Laboratory, Sagar, Madhya Pradesh, India

    Pankaj Shrivastava

  2. DNA Fingerprinting Unit, Regional Forensic Science Laboratory, Bhopal, Madhya Pradesh, India

    Hirak Ranjan Dash

  3. Department of Legal Medicine, University of Granada, Granada, Granada, Spain

    Jose A. Lorente

  4. DNA Fingerprinting Unit, State Forensic Science Laboratory, Ranchi, Jharkhand, India

    Jahangir Imam

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Rao, A., Rana, M., Pradhan, A., Sinha, M. (2020). RNA- and DNA-Based Identification of Body Fluids. In: Shrivastava, P., Dash, H.R., Lorente, J.A., Imam, J. (eds) Forensic DNA Typing: Principles, Applications and Advancements . Springer, Singapore. https://doi.org/10.1007/978-981-15-6655-4_5

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