Abstract
Safflower (Carthamus tinctorius L., Asteraceae) is an important edible oilseed crop. Because of the distinct seed oil profile, high α-tocopherol content, utilization as a leafy vegetable and useful petal pigments, it has special value among oilseed crops and is of much scientific interest. Recently, safflower has been improved for agronomical, nutritional and other traits with the introduction of specific genes from safflower and also other sources. The prerequisite for successful transformation is development of an in vitro propagation protocol, transformation method and gene of interest. Variation exists in regeneration frequency via organogenesis or somatic embryogenesis in different genotypes of safflower. Therefore, standardization of regeneration protocol is necessary for each genotype before gene transformation. Among different explants, cotyledons and apical shoot tips were found suitable for transformation and shoot regeneration. Agrobacterium-mediated transformation is the successful method for gene transfers in safflower. So far using this method, transformation has been achieved for the enhancement of γ-linolenic acid (GLA), α-linolenic acid (ALA), oleic acid, bioactive peptide, bioactive flavonoid and resistance to fungal pathogen Alternaria carthami. The commercial cultivation of genetically modified (GM) safflower is in progress in Australia, Canada and the USA. However, there is scope for improving the frequency of plant regeneration and genetic transformation. The present chapter describes the recent developments in genetic transformation and improvement of safflower.
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References
Akihisa T, Oinnma H, Tamura T (1994) Erythro-hentriacontane-6.8-dilo and 11 other alkane 6,8-dilos from Carthamus tinctorius L. Phytochemistry 36:105–108
Anjani K, Yadav P (2017) High yielding-high oleic non-genetically modified Indian safflower cultivars. Ind Crop Prod 104:7–12
Anonymous (1950) Carthamus tinctorius L. In: Wealth of India, Indian raw materials, vol 2. CSIR, Indian, Bhopal, pp 83–88
Anonymous (2019) The biology of Carthamus tinctorius L. (safflower) for information on the Australian Government Office of the Gene Technology Regulator Version 1.2, pp 14–15
Badri J, Ansari NA, Mulpuri S (2009) Plan regeneration and microprojectile-mediated transient β-glucuronidase (gus) gene expression in mature embryos of Safflower (Carthamus tinctorius L.). Asian Austr J Plant Sci Biotech 3(1):31–36
Baker CM, Dyer WE (1996a) Genetic transformation of Carthamus tinctorius L. (safflower). In: Bajaj YPS (ed) Plant protoplasts and genetic engineering. Biotechnology in agriculture and forestry. Springer Nature, Berlin, pp 201–210
Baker CM, Dyer WE (1996b) Improvements in rooting regenerated safflower (Carthamus tinctorius L.) shoots. Plant Cell Rep 16:106–110
Basalma D, Uranbey S, Mirici S, Kolsarici O (2008) TDZ×IBA induced shoot regeneration from cotyledonary leaves and in vitro multiplication in safflower. African J Biotech 7(8):960–966
Baum A (2008) Sem Bio Sys Genetics Inc. submits IND for Safflower-produced insulin to U.S.FDA. http://www.isaaa.org/kc/cropbiotechupdate/article/default.asp?ID=2905
Belide S, Hac L, Singh SP, Green AG, Wood CC (2011) Agrobacterium-mediated transformation of safflower and the efficient recovery of transgenic plants via grafting. Plant Methods 7:12
Bella SL, Tuttolomondo T, Lazzeri L, Matteo R, Leto C, Licata M (2019) An agronomic evaluation of new safflower (Carthamus tinctorius L.) germplasm for seed and oil yields under Mediterranean climate conditions. Agronomy 9(468):2–16. https://doi.org/10.3390/agronomy9080468
Chavan S, Lokhande V, Nitnaware K, Nikam T (2011) Influence of growth regulators and elicitors on cell growth and α-tocopherol and pigment productions in cell cultures of Carthamus tinctorius L. Appl Microbiol Biotechnol 89:1701–1707
Chen K, Wang Y, Zhang R, Zhang H, Gao C (2019) CRISPR/Cas genome editing and precision plant breeding in agriculture. Annu Rev Plant Biol 70:667–697
Claassen CE (1950) Natural and controlled crossing in Safflower, Carthamus tinctorius L. Agron J 42:381–384
Devi IS, Ansari NA, Kumar VD (2009) Biofortification of safflower oil with gamma linoleic acid through transgenic approach using delta 6 desaturase gene from Borago officianalis. In: 7th International Safflower Conference, Wagga Wagga, Australia
Dhumale DR, Dudhare, Mohite NR, Shingote PR, Jadhav PV, Moharil MP (2015) Refinement of in vitro regeneration system in elite safflower (Carthamus tinctorius L.) genotypes. J Plant Cell Tissue Res 15(1):4849–4854
Dhumale DR, Shingote PR, Dudhare MS, Jadhav PV, Kale PB (2016) Parameters influencing Agrobacterium-mediated transformation system in safflower genotypes AKS-207 and PKV Pink. 3 Biotech 6:181. https://doi.org/10.1007/s13205-016-0497-4
El-Lattief EA (2012) Evaluation of 25 safflower genotypes for seed and oil yields under arid environment in upper Egypt. Asia J Crop Sci 4(2):72–79
FAO (2016) Food and Agriculture Organization of the United Nations. FAOSTAT. Availability at http://www.apps.fao.org
FAO (2019) FAO Statistical Yearbook: World Food and Agriculture. Food andAgriculture Organization of the United Nations. http://www.fao.org/docrep
FDA (Food and Drug Administration) (2010) Arcadia Biosciences, Inc.; Filing of Food Additive Petition (Animal Use); Safflower Seed Meal. Federal Register 75(202)/Wednesday, October 20, 2010/Notices
FDA (Food and Drug Administration) (2015) Federal Register 80(119) Monday, June 22, 2015/rules and regulations
FSANZ (2011) Application A1049—food derived from herbicide-tolerant, high oleic acid soybean line MON87705. Assessment report. Food Standards Australia New Zealand
FSANZ (2018a) Food standards Australia New Zealand. Commercial release of genetically modified safflower. Regulation of GM Crops and foods developed using gene silencing
FSANZ (2018b) Application to food standards Australia and New Zealand for the inclusion of safflower with high oleic acid composition in standard 1.5.2 food produced using Gene Technology
Furuya T, Yoshikawa T, Kimura T, Kaneko H (1987) Production of tocopherols by cell culture of safflower. Phytochemistry 26:2741–2747
Gamborg OL, Shyluk JP (2013) Nutrition media and characteristics of plant cell and tissue culture. In: Thorpe TA (ed) Plant tissue culture: methods and applications in agriculture. Academic Press, New York, p 45
Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension culture of soybean root cells. Exp Cell Res 50:15–158
Gao W, Fan L, Paek K (2000) Yellow and red pigment production by cell cultures of Carthamus tinctorius L in a bioreactor. Plant Cell Tiss Org Cult 60:95–100
George L, Rao PS (1982) In vitro multiplication of safflower (Carthamus tinctorius L.) through tissue culture. Proc Indian Natl Sci Acad B48:791–794
Ghorbani E, Hasani Keleshteri R, Shahbazi M, Moradi F, Sadri M (2015) Optimization of extraction yield of carthamine and safflower yellow pigments from safflower (Carthamus tinctorious L.) under different treatments and solvent systems. Res J Pharmacognosy (RJP) 2(1):17–23
Govindaraj M, Vetriventhan M, Srinivasan M (2015, 2015) Importance of genetic diversity assessment in crop plants and its recent advances: an overview of its analytical perspectives. Genet Res Int:431487. https://doi.org/10.1155/2015/431487
GRDC (2010) Raising the bar with better safflower agronomy. Grains Research and Development Corporation ACT, Canberra
Guo D, Xue Y, Li D, He B, Jia X, Dong X, Guo M (2017) Overexpression of CtCHS1 increases accumulation of quinochalcone in Safflower. Front Plant Sci 8:1409. https://doi.org/10.3389/fpls.2017.01409
Haldrup A, Petersen SG, Okkels FT (1998) The xylose isomerase gene from Thermoanaerobacterium thermosulfurogenes allows effective selection of transgenic plant cells using D-xylose as the selection agent. Plant Mol Biol 37:287–296
Horrobin DF (1990) Gamma linolenic acid: an intermediate in essential fatty acid metabolism with potential as an ethical pharmaceutical and as a food. Rev Contemp Pharmacother 1:1–45
ISAAA (2017) Global status commercialized biotech/ GM crops in 2017: biotech crop adaptation surges as economic benefits accumulate in 22 years ISAAA brief no 53. Ithaca, ISAAA
Janmohammadi M, Sabaghnia N, Seifi A, Pasandi M (2017) The impacts of nano-structured nutrients on chickpea performance under supplemental irrigation. Acta Univ Agric Silvic Mendel Brun 3:859–870
Jaychandran V, Ponmanickam P, Samuel P, Sudarmani D, Pandiarajan J (2017) Influence of meta topolin on efficient plant regeneration via micropropation and organogenesis of safflower (Carthamus tinctorius L.) cv. NARI-H-15. Am J Plant Sci 8:688–705
Joersbo M, Donaldson I, Kreiberg J, Petersen SG, Brunstedt J, Okkels FT (1998) Analysis of mannose selection used for transformation of sugar beet. Mol Breed 4:111–117
Kishor PBK, Sangam S, Amrutha RN, Laxmi PS, Naidu KR, Rao KRS (2005) Regulation of proline biosynthesis, degradation, uptake and transport in higher plants: its implications in plant growth and abiotic stress tolerance. Curr Sci 88:424–438
Kumar SP, Kumari BDR (2011) Factors affecting on somatic embryogenesis of safflower (Carthamus tinctorius L) at morphological and biochemical levels. World J Agric Sci 7(2):197–205
Kumar SP, Kumari BDR (2017) Indirect somatic embryogenesis from transgenic immature leaf of safflower Carthamus tinctorius (Mohler, Roth, Schmidt & Boudreaux, 1967) (Asterales: Asteraceae). Braz J Biol Sci 4(8):247–258
Kumar JV, Kumari BR, Castano E (2008a) Cyclic somatic embryogenesis and efficient plant regeneration from callus of safflower. Biol Plantarum 52:429–436
Kumar JV, Kumari BR, Sujatha G, Castano GS (2008b) Production of plants resistant to Alternaria carthami via organogenesis and somatic embryogenesis of safflower cv. NARI-6 treated with fungal culture filtrates. Plant Cell Tiss Organ Cult 93:85–96
Kumar AM, Sundaresha S, Shreevathsa R (2009) Resistance to Alternaria leaf spot disease in transgenic safflower (Carthamus tinctorius L.) harboring a rice chitinase gene. Transgenic Plant J 3(Special issue 1):113–118
Kunze I, Ebneth M, Heim U, Geiger M, Sonnewald U, Herbers K (2001) 2-Deoxyglucose resistance: a novel selection marker for plant transformation. Mol Breed 7:221–227
Lemmon ZH, Reem NT, Dalrymple J, Soyk S, Swartwood KE, Rodriguez-Leal D, Van Eck J, Lippman ZB (2018) Rapid improvement of domestication traits in an orphan crop by genome editing. Nat Plants 4:766–770
Linsmaier EM, Skoog F (1965) Organic growth factor requirements of tobacco tissue cultures. Physiol Plant 18:100–127
Liu X, Ahmad N, Yang L, Fu T, Kong J, Na Y, Dong Y, Wang N, Li X, Wang F, Liu X, Liu W, Li H (2019) Molecular cloning and functional characterization of chalcone isomerase from Carthamus tinctorius. AMB Expr 9:132
Lloyd G, McCown B (1981) Commercially feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoot-tip culture. Comb Proc Intern Plant Prop Soc 30:421–427
Loyola-Vargas VM, Ochoa-Alejo N (2018) An introduction to plant tissue culture: advances and perspectives. Methods Mol Biol 1815:3–13
Mandal AK, Gupta SD (2001) Direct shoot organogenesis and plant regeneration in safflower. In Vitro Cell Dev Biol Plant 37:50–54
Mandal AKA, Gupta SD (2003) Somatic embryogenesis of safflower: influence of auxin and ontogeny of somatic embryos. Plant Cell Tissue Organ Cult 72:27–31
Mandal AA, Chatterji AK, Gupta SD (1995) Direct somatic embryogenesis and plantlet regeneration from cotyledonary leaves of safflower. Plant Cell Tiss Org Cult 43:287–290
Mandal AK, Gupta SD, Chatterji AK (2001) Factors affecting somatic embryogenesis from cotyledonary explants of safflower. Biol Plant 44:503–507
Matern U, Kneusel RE (1993) The use of recombinant DNA techniques to confer resistance to the Alternaria leaf spot disease of safflower. In: Li D, Yuanzhou H (eds) Proceedings of the 3rd International Safflower Conference, Beijing, June 14–18, 1993, pp 807–815
Meselhy M, Kadota S, Mimose Y, Hatakeyama N, Kusai A, Hattori M, Namba T (1993) Two new quinochalcome yellow pigments form Carthamus tictorius and Ca2+ antagonistic activity of tinctorimine. Chem Pharm Bull 41:1796–1802
Miki B, McHugh S (2004) Selectable marker genes in transgenic plants: applications, alternatives and biosafety. J Biotechnol 107(3):193–232
Mohite N, Dudhare M, Jadhav PV, Moharil MP, Deshmukh A (2014) In vitro shoot regeneration and plantlets development in Safflower (Carthamus tinctorius L.). Bioscan 9(2):551–555
Motamedi J, Zebarjadi A, Kahrizi D, Salmanian AH (2011) In vitro propagation and Agrobacterium-mediated transformation of safflower (Carthamus tinctorius L.) using a bacterial mutated aroA gene. Aust J Crop Sci 5(4):479–486
Mundel HH, Blackshaw RE, Byers JR, Huang HC, Johnson DL, Keon R, Kubik J, McKenzie R, Otto B, Roth B, Stanford K (2004) Safflower production in the Canadian prairies. Agriculture and Agri-Food Canada, Lethbridge Research Centre, Lethbridge
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–479
Murphy D (2016) Plant storage lipids. In: eLS. Wiley, Chichester. https://doi.org/10.1002/9780470015902.a0001918.pub3
Nikam T, Shitole M (1993) Regeneration of niger (Guizotia abyssinica Cass.) CV Sahyadri from seedling explants. Plant Cell Tissue Organ Cult 32:345–349
Nikam TD, Shitole MG (1999) In vitro culture of safflower L. cv. Bhima: initiation, growth optimization and organogenesis. Plant Cell Tissue Organ Cul 55:15–22
Nitsch JP, Nitsch C (1969) Haploid plants from pollen grains. Science 163:85–87
Nykiforuk CL, Shen Y, Murray EW, Boothe JG, Busseuil D, Rhéaume E, Tardif J, Reid A, Moloney MM (2011) Expression and recovery of biologically active recombinant Apolipoprotein AIMilano from transgenic safflower (Carthamus tinctorius) seeds. Plant Biotechnol J 9(2):250–263
Nykiforuk C, Shewmaker C, Harry I, Yurchenko O, Zhang M, Reed C, Oinam G, Steve Z, Fidantsef A, Boothe J, Moloney M (2012) High level accumulation of gamma linolenic acid (C18:3D6.9,12 cis) in transgenic safflower (Carthamus tinctorius) seeds. Transgenic Res 21:367–381
Omidi AH, Sharifmoghaddasi M (2010) Study of safflower varieties for flower and grain yields and fatty acid composition. Adv Environ Biol 4(3):524–527
Orlikowska TK, Dyer WE (1993) In vitro regeneration and multiplication of safflower (Carthamus tinctorius L.). Plant Sci 93:151–157
Orlikowska TK, Cranston HJ, Dyer WE (1995) Factors influencing Agrobacterium tumefaciens mediated transformation and regeneration of the safflower cultivar ‘Centennial’. Plant Cell Tiss Org Cult 40:85–91
Patial V, Krishna R, Arya G, Singh VK, Agarwal M, Goel S, Jagannath A, Kumar A (2016) Development of an efficient, genotype independent plant regeneration and transformation protocol using cotyledonary nodes in safflower (Carthamus tinctorius L.). J Plant Biochem Biotechnol 25(4):421–432
Pingali P, Aiyar A, Abraham M, Rahman A (2019) Transforming food systems for a rising India. In: Agricultural technology for increasing competitiveness of small holders, pp 215–240
Prasad BR, Khadeer MA, Seeta P, Anwar SY (1991) In vitro induction of androgenic haploids in safflower (Carthamus tinctorius L.). Plant Cell Rep 10:48–51
Radhika K, Sujatha M, Rao NT (2006) Thidiazuron stimulates adventitious shoot regeneration in different safflower explants. Biol Plant 50(2):174–179
Rani A, Panwar A, Sathe M, Alageri KC, Kush A (2018) Biofortification of safflower: an oil seed crop engineered for ALA-targeting better sustainability and plant-based omega-3 fatty acids. Transgenic Res 27(3):253–263. https://doi.org/10.1007/s11248-018-0070-5
Rao SK, Rohini VK (1999) Gene transfer into Indian cultivars of safflower (Carthamus tinctorius L.) using Agrobacterium tumefaciens. Plant Biotech 16:201–206
Rohini VK, Rao SK (2000) Embryo transformation, a practical approach for realizing transgenic plants of safflower (Carthamus tinctorius L.). Ann Bot 86:1043–1049
Rudolphi S, Becker HC, Schierolt A, von Witzke-Ehbrecht (2012) Improved estimation of oil, linoleic and oleic acid and seed hull fractions in safflower by NIRS. J Am Oil Chem Soc 89:363–369
Shah PK, Nilsson J, Kaul S, Fishbein MC, Agelan H, Hamsten A, Johansson J, Karpe F, Cercek B (1998) Effects of recombinant Apolipoprotein A-IMilano on aortic atherosclerosis in Apolipoprotein E–deficient mice. Circulation 97:780–785
Shahrokhnia M, Sepaskhah A (2017) Safflower model for simulation of growth and yield under various irrigation strategies, planting methods and nitrogen fertilization. International J Plant Prod 11(1):167–192
Shanklin J, Cahoon EB (1998) Desaturation and related modifications of fatty acids. Annu Rev Plant Physiol Plant Mol Biol 49:611–641
Shilpa KS, Dinesh Kumar V, Sujatha M (2010) Agrobacterium-mediated genetic transformation of safflower (Carthamus tinctorius L.). Plant Cell Tiss Org Cult 103:387–401. https://doi.org/10.1007/s11240-010-9792-7
Shimizu-Sato S, Tanaka M, Mori H (2009) Auxin-cytokinin interactions in the control of shoot branching. Plant Mol Biol 4:429–435
Singh S, Larkin P, Green A (2019) Fatty acids and pharmamolecules. In: Zeigler R (ed) Sustaining global food security: the nexus of science and policy. CSIRO Publishing, Canberra, pp 97–112
Smilovic M, Gleeson T, Adamowski J, Langhorn C (2019) More food with less water—optimizing agricultural water use. Adv Water Resour 123:256–261
Sujatha M, Kumar VD (2007) In vitro bud regeneration of Carthamus tinctorius and wild Carthamus species from leaf explants and axillary buds. Biol Plantarum 51:782–786
Surbhaiyya S, Dudhare M, Thakre R, Jahav P, Moharil M, Dhumale D, Umbarkar H (2018) In vitro callus induction from two different explants cotyledonary leaves and hypocotyle in Carthamus tinctorius Linn. var pkv-pink. Indian Res J Genet Biotech 10(1):37–43
Tejovathi G, Anwar SY (1984) In vitro induction of capitula from cotyledons of Carthamus tinctorius (safflower). Plant Sci 36:165–168
Tejovathi G, Anwar SY (1993) 2,4,5-Trichloro phenoxy propionic acid induced rhizogenesis in Carthamus tinctorius L. Proc Indian Nat Sci Acad B59(6):633–636
Topfer R, Martini N, Schell J (1995) Modification of plant lipid synthesis. Science 268:681–686
Villanueva-Mejia D, Alvarez JC (2017) Genetic improvement of oilseed crops using modern biotechnology. In: Advances in seed biology. Web of Science™ Core Collection (BKCI), pp 295–317
Walia N, Amandeep K, Babbar SB (2005) In vitro regeneration of a high oil-yielding variety of safflower (Carthamus tinctorius var HUS-305). J. Plant Biochem Biotechnol 14(1):65–68
Walia N, Kaur A, Babbar SB (2007) Proliferation and differentiation from endosperms of Carthamus tinctorius. Biol Plant 51(4):749–753
Wood C, Okada S, Taylor M, Menon A, Mathew A, Cullerne D, Stephen SJ, Allen R, Zhou X, Liu Q, Oakeshott J, Singh S, Green A (2018) Seed-specific RNAi in safflower generates a superhigh oleic oil with extended oxidative stability. Plant Biotechnol J 16:1788–1796
Yang J, Xiong L, Li T (2009) The effect of phytohormones on safflower regeneration plant. J Chinese Med Mater 32:1335–1338
Yelchuri V, Srikanth K, Prasad RBN, Karuna MSL (2019) Olefin metathesis of fatty acids and vegetable oils. J Chem Sci 131:39. https://doi.org/10.1007/s12039-019-1615-8
Ying M, Dyer WE, Bergman JW (1992) Agrobacterium tumefaciens–mediated transformation of safflower (Carthamus tinctorius L.) cv “Centennial”. Plant Cell Rep 11:581–585
Zhou X, Tang L, Xu Y, Zhou G, Wang Z (2014) Towards a better understanding of medicinal uses of Carthamus tinctorius L. in traditional Chinese medicine: a phytochemical and pharmacological review. J Ethanopharmacol 151(1):27–43
Zhu LH, Krens F, Smith MA, Li X et al (2016) Dedicated industrial oilseed crops as metabolic engineering platforms for sustainable industrial feedstock production. Sci Rep 6:22181
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Nitnaware, K.M. et al. (2021). Genetic Engineering in Safflower (Carthamus tinctorius L.): Retrospect and Prospect. In: Kavi Kishor, P.B., Rajam, M.V., Pullaiah, T. (eds) Genetically Modified Crops. Springer, Singapore. https://doi.org/10.1007/978-981-15-5897-9_10
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