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Molecular Biology Techniques for the Identification and Genotyping of Microorganisms

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Microbial Genomics in Sustainable Agroecosystems

Abstract

The advent in molecular biology techniques has enabled, to a great extent, numerous identification and detection techniques of microorganisms by amplifying specific conserved DNA sequences. From time-to-time, the tremendous modifications have been employed in search of a rapid and inexpensive microbial identification method and now a whole genome to be sequenced is possible. In this chapter, we have described the different molecular identification techniques including 16S/18S rRNA, ITS and whole-genome sequencing as well as genotyping techniques such as pulse field gel electrophoresis, AFLP, RAPD, RFLP, ribotyping, BOX, ERIC, rep-PCR and multi-locus sequence typing with their basic principle, procedure, strengths and weaknesses.

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Acknowledgments

This work was supported by Puri Foundation for Education in India.

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  1. Vijai Singh

    Present address: Department of Biosciences, School of Sciences, Indrashil University, Kadi, Gujarat, India

Authors and Affiliations

  1. School of Biological Sciences and Biotechnology, Institute of Advanced Research, Gandhinagar, India

    Nisarg Gohil, Happy Panchasara, Shreya Patel & Vijai Singh

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Editors and Affiliations

  1. Department of Molecular and Cellular Engineering, Jacob Institute of Biotechnology and Bioengineering, Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj, Uttar Pradesh, India

    Vijay Tripathi

  2. Department of Forestry, North Eastern Regional Institute of Science and Technology (Deemed To Be University-MHRD), Itanagar, Arunachal Pradesh, India

    Pradeep Kumar

  3. Department of Computational Biology and Bioinformatics, Jacob Institute of Biotechnology and Bioengineering, Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj, Uttar Pradesh, India

    Pooja Tripathi

  4. Department of Botany, Kamla Nehru P.G. College, Raebareli, Uttar Pradesh, India

    Amit Kishore

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Gohil, N., Panchasara, H., Patel, S., Singh, V. (2019). Molecular Biology Techniques for the Identification and Genotyping of Microorganisms. In: Tripathi, V., Kumar, P., Tripathi, P., Kishore, A. (eds) Microbial Genomics in Sustainable Agroecosystems. Springer, Singapore. https://doi.org/10.1007/978-981-13-8739-5_11

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