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Nucleic Acid Amplification Strategy-Based Colorimetric Assays

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Nucleic Acid Amplification Strategies for Biosensing, Bioimaging and Biomedicine

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Abstract

Colorimetric assays , using colored substances in solution to determine the concentration without the aid of advanced instrumentation, are a convenient, cost-effective, and rapid method for ions, small molecules , oligonucleotides, miRNA , and protein detections. Nucleic acid amplification strategies play an important role in highly sensitive detection. Based on nucleic acid amplification , colorimetric assays detections would be more sensitive, proved by many detections, such as DNA, miRNA, and tumor cells . In this chapter, we introduce the nucleic acid amplification strategy-based colorimetric assays, including various amplification methods and colored substances. Au nanoparticles and Ag nanoparticles are usually employed as the colored substances by aggregation . HRP-catalyzed conversion of ABTS and TMB is also used as colored substances, which can be oxidized by hydrogen peroxide with color change , widely applied in electrochemical analysis and detection. The nucleic acid amplification strategies contain enzyme-assisted (PCR , RCA , NEANA, EASA, LAMP, DSN, NASBA, ESPAR, HDA) and enzyme-free (HCR, SDA ). Additionally, the future perspectives and advantages of adoption nucleic acid amplification techniques in colorimetric assays are also discussed.

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Authors and Affiliations

  1. Shandong Provincial Key Laboratory of Detection Technology for Tumour Markers, College of Chemistry and Chemical Engineering, Linyi University, Linyi, 276005, People’s Republic of China

    Pengfei Shi & Xiangjiang Zheng

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  1. Pengfei Shi
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  2. Xiangjiang Zheng
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Correspondence to Pengfei Shi .

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Editors and Affiliations

  1. Shandong Provincial Key Laboratory of Detection Technology for Tumour Markers, Linyi University, Linyi, Shandong, China

    Shusheng Zhang

  2. College of Chemistry and Chemical Engineering, Qingdao University, Qingdao, Shandong, China

    Sai Bi

  3. Shandong Provincial Key Laboratory of Detection Technology for Tumour Markers, College of Chemistry and Chemical Engineering, Linyi University, Linyi, Shandong, China

    Xinyue Song

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Shi, P., Zheng, X. (2019). Nucleic Acid Amplification Strategy-Based Colorimetric Assays. In: Zhang, S., Bi, S., Song, X. (eds) Nucleic Acid Amplification Strategies for Biosensing, Bioimaging and Biomedicine. Springer, Singapore. https://doi.org/10.1007/978-981-13-7044-1_5

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