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Mass Production of Lilium Bulblets in Bioreactors

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Production of Biomass and Bioactive Compounds Using Bioreactor Technology

Abstract

Lilium is an important floricultural crop for bulb and cut flower production. Culturing Lilium bulblets from bulbscales is one of the main methods for vegetative propagation; however, the traditional tissue culture requires numerous vessels and considerable labour. This study evaluates the efficiency of using air-lift bioreactor systems to produce Lilium bulblets from bulbscale segments. The factors that affect bulblet formation and enlargement in vitro and specific factors that affect bulblet production in air-lift bioreactor systems were investigated. A simple method to mass produce bulblets by using a one-step bioreactor culture is indicated, and a pilot-scale bioreactor culture is introduced. The effects of storage temperature and duration on the carbohydrates and related enzymes of in vitro–produced bulblets are determined during breaking of dormancy. A suitable bulblet size for culturing is determined after observing the emergence of cauline leaves from different sized bulblets that are produced in solid and bioreactor cultures. Finally, an efficient method to mass produce high-quality Lilium bulblets is selected based on the production cost.

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Abbreviations

BA:

6-Benzyladenine

BTBB:

Balloon-type bubble bioreactor

DIF:

Difference

LEDs:

Light emitting diodes

MS:

Murashige-Skoog

NAA:

α-Naphthalene acetic acid

SPS:

Sucrose phosphate synthase

SS:

Sucrose synthase

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Acknowledgments

This work was supported by Korea Science and Engineering Foundation (KOSEF) through Research Center for the Development of Advanced Horticultural Technology at Chungbuk National University, Cheongju, 361–763, Korea and National Science Foundation of China (30860176).

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Authors and Affiliations

  1. Key Laboratory of Nature Resource of Changbai Mountain and Functional Molecular, Yanbian University, Ministry of Education, Park Road 977, 133002, Yanji, Jilin, China

    Mei-Lan Lian & Xuan-Chun Piao

  2. Department of Horticultural Science, Chungbuk National University, 361-763, Cheongju, Republic of Korea

    So-Young Park

Authors
  1. Mei-Lan Lian
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  2. Xuan-Chun Piao
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  3. So-Young Park
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Corresponding author

Correspondence to Mei-Lan Lian .

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Editors and Affiliations

  1. Department of Horticultural Science, Chungbuk National University, Cheongju, Korea, Republic of (South Korea)

    Kee-Yoeup Paek

  2. Department of Botany, Karnatak University, Dharwad, India

    Hosakatte Niranjana Murthy

  3. School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai, China

    Jian-Jiang Zhong

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Lian, ML., Piao, XC., Park, SY. (2014). Mass Production of Lilium Bulblets in Bioreactors. In: Paek, KY., Murthy, H., Zhong, JJ. (eds) Production of Biomass and Bioactive Compounds Using Bioreactor Technology. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-9223-3_16

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