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Accumulation of Okadaic Acid and Detoxifying Enzymes in the Digestive Gland of Mytilus galloprovincialis During Exposure to DSP

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Molluscan Shellfish Safety

Abstract

Little is known about the metabolism of toxins in mussels, although the involvement of detoxifying enzymes, as well as of antioxidant enzymes, has been suggested, because the induction of some of them has been observed in shellfish exposed to those compounds.

In this preliminary study we analyse the activity of cytochrome P450 reductase, which plays an essential role in the transformation of liposoluble xenobiotics, the activity of some antioxidant enzymes (glutathione reductase, glutathione peroxidase, glutathione-S-transferase, catalase). Other detoxification enzymes including the glyoxalase system, which is involved in detoxification of reactive α-keto aldehydes generated in cellular oxidative processes, are also included. The variation of these enzymatic activities, were analysed in relation to OA (okadaic acid) accumulation during three separate toxic blooms of Dinophysis acuminata and Dinophysis acuta in the Ria de Vigo. An inverse relation between OA and some of these enzymatic activities was observed, which suggests the induction/activation of these enzymes by OA, and the subsequent decrease of the toxin due to its metabolism.

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Acknowledgment

The authors thank Antonio Antepazos and the fishermen of the “Antepazos I”, who kindly provided the mussels used in this work. This work was supported by a grant from Autonomous Galician Government (Ref. 2008/cp390) within the Strategic Action: EPITOX.

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Authors and Affiliations

  1. Department of Biochemistry, Genetics and Immunology, University of Vigo, Vigo, Spain

    A. Vidal, Y. Ruiz, P. Suárez & F. San Juan

  2. Centro de Investigacións Mariñas (Xunta de Galicia), Pontevedra, Spain

    A. E. Rossignoli & J. Blanco

  3. Department of Biochemistry and Molecular Biology, University of Santiago de Compostela, Santiago de Compostela, Spain

    O. Garcia

  4. Department of Biochemistry, Genetics and Immunology, Faculty of Sciences, University of Vigo, Lagoas-Marcosende s/n, 36310, Vigo, Spain

    Ana Alonso Martinez

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  1. A. Vidal
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Correspondence to F. San Juan .

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  1. Canadian Food Inspection Agency, Ottawa, Ontario, Canada

    Gilbert Sauvé

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Vidal, A. et al. (2014). Accumulation of Okadaic Acid and Detoxifying Enzymes in the Digestive Gland of Mytilus galloprovincialis During Exposure to DSP. In: Sauvé, G. (eds) Molluscan Shellfish Safety. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-6588-7_19

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