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Fluorescence Lifetime Imaging

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Handbook of Photonics for Biomedical Engineering

Abstract

Fluorescence lifetime imaging (FLIM) is a key fluorescence microscopy technique to map the environment and interaction of fluorescent probes. It can report on photophysical events that are difficult or impossible to observe by fluorescence intensity imaging, because FLIM is largely independent of the local fluorophore concentration and excitation intensity. Many FLIM applications relevant for biology concern the identification of Förster resonance energy transfer (FRET) to study protein interactions and conformational changes. In addition, FLIM has been used to image viscosity, temperature, pH, refractive index, and ion and oxygen concentrations, all at the cellular level. The basic principles and recent advances in the application of FLIM, FLIM instrumentation, molecular probe, and FLIM detector development will be discussed.

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Acknowledgments

We would like to thank the UK’s MRC, BBSRC, and EPSRC for funding.

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Authors and Affiliations

  1. Department of Physics, King’s College London, Strand, London, WC2R 2LS, UK

    Klaus Suhling, Liisa M. Hirvonen, James A. Levitt, Pei-Hua Chung & Carolyn Tregidgo

  2. Institute of Neurology, University College London, Queen Square, London, WC1N 3BG, UK

    Dmitri A. Rusakov & Kaiyu Zheng

  3. Randall Division of Cell and Molecular Biophysics, King’s College London, London, SE1 1UL, UK

    Simon Ameer-Beg & Simao Coelho

  4. Richard Dimbleby Department of Cancer Research, Division of Cancer Studies, New Hunt’s House, King’s College London, Guy’s Campus, London, SE1 1UL, UK

    Simon Ameer-Beg, Simon P. Poland & Simao Coelho

  5. CMOS Sensors and Systems Group, Integrated Micro and Nano Systems, School of Engineering, University of Edinburgh, Edinburgh, EH9 3JL, UK

    Robert Henderson & Nikola Krstajic

  6. EPSRC IRC “Hub” in Optical Molecular Sensing and Imaging, MRC Centre for Inflammation Research, Queen’s Medical Research Institute, University of Edinburgh, 47 Little France Crescent, Edinburgh, EH16 4TJ, UK

    Nikola Krstajic

  7. Division of Cancer Research and Randall Division of Cell and Molecular Biophysics, Guy’s Campus, King’s College London, London, WC2R 2LS, UK

    Simon P. Poland

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  1. Klaus Suhling
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  2. Liisa M. Hirvonen
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  7. Kaiyu Zheng
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  8. Simon Ameer-Beg
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  9. Simon P. Poland
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  10. Simao Coelho
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  11. Robert Henderson
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  12. Nikola Krstajic
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Corresponding author

Correspondence to Klaus Suhling .

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Editors and Affiliations

  1. Department of Electronic Engineering, The Chinese University of Hong Kong, Shatin, Hong Kong

    Aaron Ho-Pui Ho

  2. School of Electrical and Electronic Engineering, Yonsei University, Seoul, Korea (Republic of)

    Donghyun Kim

  3. Department of Electronic and Information Engineering, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong

    Michael G. Somekh

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Suhling, K. et al. (2017). Fluorescence Lifetime Imaging. In: Ho, AP., Kim, D., Somekh, M. (eds) Handbook of Photonics for Biomedical Engineering. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-5052-4_13

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