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Recent Development of In Vivo Cryotechnique to Cryobiopsy for Living Animals

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In Vivo Cryotechnique in Biomedical Research and Application for Bioimaging of Living Animal Organs

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Abstract

Tissue resection or perfusion fixation in conventional procedures causes ischemic or anoxic artifacts. In vivo cryotechnique (IVCT) has been used to overcome these problems and study the distribution of soluble molecules without ischemic/anoxic artifacts at high time resolution. There are some limitations of IVCT; the target organs of living small animals need to be exposed, and tissues adjacent to the target organs are inevitably damaged by poured isopentane-propane cryogens. A new cryotechnique, “cryobiopsy,” enables acquisition of tissue specimens of larger animals without anoxia/ischemia with technical advantages similar to those of IVCT. Live-imaging techniques could be complemented by IVCT and cryobiopsy, and cryofixation can preserve all components in frozen tissue specimens. Thus, IVCT and cryobiopsy followed by freeze substitution for light or electron microscopy will provide more precise morphofunctional information in vivo on tissue sections, and also they can be combined with other analytical methods, such as Raman microscopy, freeze-fracture replication, and X-ray microanalyses. The merits of IVCT and cryobiopsy will be underscored in the applications to other microscopic fields and experimental animal studies in clinical medicine.

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Authors and Affiliations

  1. Department of Anatomy and Molecular Histology, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, 1110 Shimokato, Chuo City, Yamanashi, 409-3898, Japan

    Nobuhiko Ohno M.D., Ph.D., Nobuho Terada & Shinichi Ohno

  2. Division of Health Sciences, Shinshu University Graduate School of Medicine, 3-1-1 Asahi, Matsumoto City, Nagano, 390-8621, Japan

    Nobuho Terada

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  1. Nobuhiko Ohno M.D., Ph.D.
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  2. Nobuho Terada
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  3. Shinichi Ohno
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Correspondence to Nobuhiko Ohno M.D., Ph.D. .

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Editors and Affiliations

  1. Department of Anatomy and Molecular Histology Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Yamanashi, Japan

    Shinichi Ohno

  2. Department of Anatomy and Molecular Histology Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Yamanashi, Japan

    Nobuhiko Ohno

  3. Division of Health Science, Shinshu University Graduate School of Medicine, Nagano, Japan

    Nobuo Terada

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Ohno, N., Terada, N., Ohno, S. (2016). Recent Development of In Vivo Cryotechnique to Cryobiopsy for Living Animals. In: Ohno, S., Ohno, N., Terada, N. (eds) In Vivo Cryotechnique in Biomedical Research and Application for Bioimaging of Living Animal Organs. Springer, Tokyo. https://doi.org/10.1007/978-4-431-55723-4_48

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  • DOI: https://doi.org/10.1007/978-4-431-55723-4_48

  • Publisher Name: Springer, Tokyo

  • Print ISBN: 978-4-431-55722-7

  • Online ISBN: 978-4-431-55723-4

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