Abstract
Four types of mammalian sialidase so far identified demonstrate great variation in their expression during cell differentiation, cell growth, and malignant transformation. In addition, there are accumulating evidences that they are involved in many important biological processes. To understand the biological function and altered expression under the cellular various conditions, estimation of their endogenous expression levels is definitely necessary. However, it might not be easy especially to get endogenous activity level, because there is redundancy in substrate specificity among them, even with combination of measurement of mRNA level by RT-PCR. To obtain the level as exactly as possible, here we introduce several methods of sialidase assays often employed, together with a representative example of the experiment.
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Miyagi, T., Takahashi, K., Shiozaki, K., Yamaguchi, K. (2015). Mammalian Sialidase Assays. In: Taniguchi, N., Endo, T., Hart, G., Seeberger, P., Wong, CH. (eds) Glycoscience: Biology and Medicine. Springer, Tokyo. https://doi.org/10.1007/978-4-431-54841-6_189
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DOI: https://doi.org/10.1007/978-4-431-54841-6_189
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Publisher Name: Springer, Tokyo
Print ISBN: 978-4-431-54840-9
Online ISBN: 978-4-431-54841-6
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