Abstract
Growth of animals is the complex result of competition between anabolic and catabolic process, which implies constant changes and remodeling through synthesis of new proteins and breakdown of existing proteins (Jobgen et al. 2006; Tan et al.,2009). Together, these processes are called protein turnover and produce muscle growth or hypertrophy when synthesis is greater than breakdown and muscle wasting when synthesis is less than breakdown (Norton and Layman 2006). Protein turnover requires large amounts of ATP. However, this costly metabolic cycle fulfills key obligatory functions, including protein homeostasis, cell turnover, removal of aged and damaged proteins, synthesis of new proteins like heat-shock and immunological proteins, gluconeogenesis from amino acids, wound healing, tissue repair, adaptation to nutritional and pathological alterations, and immune responses (Wu 2009). In pigs, although the protein syntheses increase in all tissues, the greatest response occurs in skeletal muscle in response to feeding stimulation. The elevated postprandial protein synthesis in skeletal muscle of pigs therefore increases the protein deposition during the post-absorptive period allowing growth and development. A sharp increase of circulating glucose, insulin, and amino acids, especially some nutritional indispensable amino acids, is observed after meal (Yin et al. 2010, 2011), in accordance with higher protein deposition in pigs (Drew et al. 2012). The mechanism responsible for the stimulation of protein synthesis by feeding was therefore focused on the roles of postprandial circulating glucose, insulin, and amino acids. The protein degradation is a process where proteins are broken into smaller peptides as well as free amino acids, the latter being either reused for new protein synthesis or further degraded into several metabolites, several of them being able to generate ATP for energy use. In addition, the protein degradation plays important roles in animal physiological process, especially in the cellular signal transduction system as well as in the maintenance of the integrity of the proper folded state of protein.
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References
Avruch J, Hara K, Lin Y, Liu M, Long X, Ortiz-Vega S, Yonezawa K (2006) Insulin and amino-acid regulation of mTOR signaling and kinase activity through the Rheb GTPase. Oncogene 25(48):6361–6372
Baehrecke EH (2005) Autophagy: dual roles in life and death? Nat Rev Mol Cell Biol 6(6):505–510
Bates PC, Millward DJ (1981) Characteristics of skeletal muscle growth and protein turnover in a fast-growing rat strain. Br J Nutr 46(1):7–13
Baumeister W, Walz J, Proteolysis C (1998) The proteasome: paradigm review of a self-compartmentalizing protease. Cell 92:367–389
Bergen WG, Wu G (2009) Intestinal nitrogen recycling and utilization in health and disease. J Nutr 139(5):821–825
Burrin DG, Shulman RJ, Reeds PJ, Davis TA, Gravitt KR (1992) Porcine colostrum and milk stimulate visceral organ and skeletal muscle protein synthesis in neonatal piglets. J Nutr 122(6):1205–1213
Bush JA, Kimball SR, O’Connor PMJ, Suryawan A, Orellana RA, Nguyen HV, Jefferson LS, Davis TA (2003) Translational control of protein synthesis in muscle and liver of growth hormone-treated pigs. Endocrinology 144(4):1273–1283
Davis TA (2008) Insulin and amino acids are critical regulators of neonatal muscle growth. Nutrition today 43(4):143–149
Davis TA, Nguyen HV, Suryawan A, Bush JA, Jefferson LS, Kimball SR (2000) Developmental changes in the feeding-induced stimulation of translation initiation in muscle of neonatal pigs. Am J Physiol Endocrinol Metab 279(6):E1226–E1234
Davis TA, Fiorotto ML, Beckett PR, Burrin DG, Reeds PJ, Wray-Cahen D, Nguyen HV (2001) Differential effects of insulin on peripheral and visceral tissue protein synthesis in neonatal pigs. Am J Physiol Endocrinol Metab 280(5):E770–E779
Davis TA, Suryawan A, Orellana RA, Nguyen HV, Fiorotto ML (2008) Postnatal ontogeny of skeletal muscle protein synthesis in pigs. J Anim Sci 86(14 suppl):E13–E18
Deng D, Yao K, Chu WY, Li TJ, Huang RL, Yin YL, Liu HQ, Zhang JS, Wu GY (2009) Impaired translation initiation activation and reduced protein synthesis in weaned piglets fed a low-protein diet. J Nutr Biochem 20:544–552
Deng J, Wu X, Bin S, Li TJ, Huang R, Liu Z, Liu Y, Ruan Z, Deng Z, Hou Y, Yin YL (2010) Dietary amylose and amylopectin ratio and resistant starch content affects plasma glucose, lactic acid, hormone levels 1 and protein synthesis in splanchnic tissues. Z Tierphysiol Tierernahr Futtermittelkd 94:220–226
Di Guglielmo GM, Baass PC, Authier F, Posner BI, Bergeron JJM (1998) Insulin receptor internalization and signalling. Mol Cell Biochem 182(1):59–63
Drew MD, Schafer TC, Zijlstra RT (2012) Glycemic index of starch affects nitrogen retention in grower pigs. J Anim Sci 90(4):1233–1241
Glickman MH, Ciechanover A (2002) The ubiquitin-proteasome proteolytic pathway: destruction for the sake of construction. Physiol Rev 82(2):373–428
Goberdhan DCI, Wilson C (2003) PTEN: tumour suppressor, multifunctional growth regulator and more. Hum Mol Genet 12(suppl 2):R239–R248
Goll DE, Thompson VF, Li H, Wei W, Cong J (2003) The calpain system. Physiol Rev 83(3):731–801
Gronostajski RM, Goldberg AL, Pardee AB (1984) The role of increased proteolysis in the atrophy and arrest of proliferation in serum-deprived fibroblasts. J Cell Physiol 121(1):189–198
Jeyapalan AS, Orellana RA, Suryawan A, O’Connor PMJ, Nguyen HV, Escobar J, Frank JW, Davis TA (2007) Glucose stimulates protein synthesis in skeletal muscle of neonatal pigs through an AMPK- and mTOR-independent process. American Journal of Physiology - Endocrinology And Metabolism 293(2):E595–E603
Jobgen WS, Fried SK, Fu WJ, Meininger CJ, Wu G (2006) Regulatory role for the arginine–nitric oxide pathway in metabolism of energy substrates. J Nutr Biochem 17(9):571–588
Kimball S (2007) The role of nutrition in stimulating muscle protein accretion at the molecular level. Biochem Soc Trans 35:1298–1301
Kimball SR, Jefferson LS (2004) Molecular mechanisms through which amino acids mediate signaling through the mammalian target of rapamycin. Curr Opin Clin Nutr Metab Care 7(1):39–44
Kimball SR, Jefferson LS, Nguyen HV, Suryawan A, Bush JA, Davis TA (2000) Feeding stimulates protein synthesis in muscle and liver of neonatal pigs through an mTOR-dependent process. Am J Physiol Endocrinol Metab 279(5):E1080–E1087
Kong XF, Tan BE, Yin YL, Li XL, Jaeger LA, Bazer FW, Wu GY (2012) Arginine stimulates the mTOR signaling pathway and protein synthesis in porcine trophectoderm cells. J Nutr Biochem 23(9):1178–1183
Lecker SH, Goldberg AL, Mitch WE (2006) Protein degradation by the ubiquitin–proteasome pathway in normal and disease states. J Am Soc Nephrol 17:1807–1819
Li F, Yin Y, Tan B, Kong X, Wu G (2011) Leucine nutrition in animals and humans: mTOR signaling and beyond. Amino Acids 41:1185–1193
Liu JG, Zhang P, Bin SY (2007) Effects of different dietary starch constituents on level of blood glucose and insulin of weaned piglets (article in Chinese). Food Sci 28:315–319 (in Chinese)
Long X, Lin Y, Ortiz-Vega S, Yonezawa K, Avruch J (2005) Rheb binds and regulates the mTOR kinase. Curr Biol 15(8):702–713
Norton LE, Layman DK (2006) Leucine regulates translation initiation of protein synthesis in skeletal muscle after exercise. J Nutr 136(2):533S–537S
O’Connor PMJ, Bush JA, Suryawan A, Nguyen HV, Davis TA (2003a) Insulin and amino acids independently stimulate skeletal muscle protein synthesis in neonatal pigs. Am J Physiol Endocrinol Metab 284(1):E110–E119
O’Connor PM, Kimball SR, Suryawan A, Bush JA, Nguyen HV, Jefferson LS, Davis TA (2003b) Regulation of translation initiation by insulin and amino acids in skeletal muscle of neonatal pigs. Am J Physiol Endocrinol Metab 285(1):E40–E53
O’Connor PM, Kimball SR, Suryawan A, Bush JA, Nguyen HV, Jefferson LS, Davis TA (2004) Regulation of neonatal liver protein synthesis by insulin and amino acids in pigs. Am J Physiol Endocrinol Metab 286(6):E994–E1003
Regmi PR, Matte JJ, van Kempen T, Zijlstra RT (2010) Starch chemistry affects kinetics of glucose absorption and insulin response in swine. Livest Sci 134(1–3):44–46
Salvesen GS, Dixit VM (1997) Caspases: intracellular signaling by proteolysis. Cell 91(4):443–446
Sarbassov DD, Guertin DA, Ali SM, Sabatini DM (2005) Phosphorylation and regulation of Akt/PKB by the rictor-mTOR complex. Science 307(5712):1098–1101
Saucedo LJ, Gao X, Chiarelli DA, Li L, Pan D, Edgar BA (2003) Rheb promotes cell growth as a component of the insulin/TOR signalling network. Nat Cell Biol 5(6):566–571
Stoll B, Burrin DG (2006) Measuring splanchnic amino acid metabolism in vivo using stable isotopic tracers. J Anim Sci 84(13 suppl):E60–E72
Suryawan A, Nguyen HV, Bush JA, Davis TA (2001) Developmental changes in the feeding-induced activation of the insulin-signaling pathway in neonatal pigs. Am J Physiol Endocrinol Metab 281(5):E908–E915
Suryawan A, Orellana RA, Nguyen HV, Jeyapalan AS, Fleming JR, Davis TA (2007) Activation by insulin and amino acids of signaling components leading to translation initiation in skeletal muscle of neonatal pigs is developmentally regulated. Am J Physiol Endocrinol Metab 293(6):E1597–E1605
Suryawan A, Jeyapalan AS, Orellana RA, Wilson FA, Nguyen HV, Davis TA (2008) Leucine stimulates protein synthesis in skeletal muscle of neonatal pigs by enhancing mTORC1 activation. Am J Physiol Endocrinol Metab 295(4):E868–E875
Tan B, Yin YL, Liu ZQ, Li XG, Xu HJ, Kong XF, Huang RL, Tang WJ, Shinzato I, Smith SB, Wu GY (2009) Dietary L-arginine supplementation increases muscle gain and reduces body fat mass in growing-finishing pigs. Amino Acids 37:169–175
Tremblay F, Gagnon AM, Veilleux A, Sorisky A, Marette A (2005) Activation of the mammalian target of rapamycin pathway acutely inhibits insulin signaling to Akt and glucose transport in 3 T3-L1 and human adipocytes. Endocrinology 146(3):1328–1337
Wang X, Proud CG (2006) The mTOR pathway in the control of protein synthesis. Physiology (Bethesda) 21:362–369
Wang L, Rhodes CJ, Lawrence JC (2006) Activation of mammalian target of rapamycin (mTOR) by insulin is associated with stimulation of 4EBP1 binding to dimeric mTOR complex 1. J Biol Chem 281(34):24293–24303
Watford M (2008) Glutamine metabolism and function in relation to proline synthesis and the safety of glutamine and proline supplementation. J Nutr 138(10):2003S–2007S
Wek RC, Jiang HY, Anthony TG (2006) Coping with stress: eIF2 kinases and translational control. Biochem Soc Trans 34(1):7–11
Wilson FA, Suryawan A, Orellana RA, Kimball SR, Gazzaneo MC, Nguyen HV, Fiorotto ML, Davis TA (2009) Feeding Rapidly Stimulates Protein Synthesis in Skeletal Muscle of Neonatal Pigs by Enhancing Translation Initiation. J Nutr 139(10):1873–1880
Wu G (1998) Intestinal mucosal amino acid catabolism. J Nutr 128(8):1249–1252
Wu G (2009) Amino acids: metabolism, functions, and nutrition. Amino Acids 37(1):1–17
Wu G (2010) Recent advances in swine amino acid nutrition. J Anim Sci Biotechnol 1:49–61
Yao K, Yin Y, Chu W, Liu Z, Deng D, Li T, Huang R, Zhang J, Tan B, Wang W, Wu G (2008) Dietary Arginine Supplementation Increases mTOR Signaling Activity in Skeletal Muscle of Neonatal Pigs. J Nutr 138(5):867–872
Yao K, Guan S, Li TJ, Yin YL (2011) Dietary L-arginine supplementation enhances intestinal development and expression of vascular endothelial growth factor in weanling piglets. Br J Nutr 105:703–709
Yao K, Wang L, Ding BY, Fu DB, Liu YL, Zhu HL, Liu J, Li YT, Kang P, Yin YL, Wu GY, Hou YQ (2012) Alpha-ketoglutarate inhibits glutamine degradation and enhances protein synthesis in intestinal porcine epithelial cells. Amino Acids 42(6):2491–2500
Yin F, Zhang Z, Huang J, Yin Y (2010) Digestion rate of dietary starch affects systemic circulation of amino acids in weaned pigs. Br J Nutr 103:1404–1412
Yin F, Yin Y, Zhang Z, Xie M, Huang J, Huang R, Li T (2011) Digestion rate of dietary starch affects the systemic circulation of lipid profiles and lipid metabolism-related gene expression in weaned pigs. Br J Nutr 106:369–377
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Yin, F., Yin, Y., Hou, Y. (2013). Synthesis and Degradation of Proteins in Pigs. In: Blachier, F., Wu, G., Yin, Y. (eds) Nutritional and Physiological Functions of Amino Acids in Pigs. Springer, Vienna. https://doi.org/10.1007/978-3-7091-1328-8_8
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