Abstract
10-HDA (10-hydroxy-2-decenoic acid), an unsaturated fatty acid, is the main active component of royal jelly. It has been demonstrated that 10-HDA possesses remarkable antibacterial activities. However, it is unclear in terms of the underlying antibacterial mechanism of 10-HDA. In our study, we showed that 10-HDA has obvious effects against Gram-negative bacteria and its antibacterial activity was not affected by pH and temperature. 10-HDA was shown to change membrane ionic conductivity and resulted in the leakage of K+, Ca2+, and Mg2+ from bacterial cells, suggesting that 10-HDA was able to compromise the integrity of the bacterial membranes. By detecting the 260 nm absorbing materials, it was showed that the membrane integrity was damaged and resulted in the leakage of large molecules. The results suggested that 10-HDA could damage the permeability and integrity of bacterial cell membrane, leading to the outflow of important large molecules from the cell and its eventual death.
Xiaohui Yang and Tengfei Wang contributed equally to this article.
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References
Blum MS, Novak AF, Taber S (1959) 10-Hydroxy-delta 2-decenoic acid, an antibiotic found in royal jelly. Science 130:452–453
Melliou E, Chinou I (2005) Chemistry and bioactivity of royal jelly from Greece. J Agric Food Chem 53:8987–8992
Vucevica D, Mellioub E, Vasilijica S et al (2007) Fatty acids isolated from royal jelly modulate dendritic cell-mediated immune response in vitro. Int Immunopharmacol 7:1211–1220
Nagai T, Inoue R, Suzuki N, Nagashima T (2006) Antioxidant properties of enzymatic hydrolysates from royal jelly. J Med Food 9:363–367
Nagai T, Sakai M, Inoue R et al (2001) Antioxidative activities of some commercially honeys, royal jelly and propolis. Food Chem 75:237–240
Satomi KM, Iwao O, Shimpei U et al (2004) Identification of a collagen production-promoting factor from an extract of royal jelly and its possible mechanism. Bioscience, Biotechnol Biochem 68:767–773
Park HM, Wang EH, Lee KG et al (2011) Royal jelly protects against ultraviolet β–induced photoaging in human skin fibroblasts via enhancing collagen production. J Med Food 14:899–906
Townsend GF, Morgan JF, Hazlett B (1959) Activity of 10-hydroxydecenoic acid from Royal Jelly against experimental leukaemia and ascitic tumours. Nature 183:1270–1271
Townsend GF, Morgan JF, Toinai S et al (1960) Studies on the in vitro antitumor activity of fatty acids 10-Hydroxy-2-decenoic acid from royal jelly. Cancer Res 20:503–510
Nakaya M, Onda H, Sasaki K (2007) Effect of royal jelly on bisphenol A-induced proliferation of human breast cancer cells. Biosciences, Biotechnol Biochem 71:253–255
Boukraa L, Meslem A, Benhanifia M et al (2009) Synergistic effect of starch and royal jelly against Staphylococcus aureus and Escherichia coli. J Altern Complem Med 15:755–757
Eshraghi S, Seifollahi F (2003) Antibacterial effects of royal jelly on different strains of bacteria. Iran J Public Health 32:25–30
Mccleskey CS, Melampy RM (1939) Bactericidal properties of royal jelly of the honeybee. J Econ Entomol 32:581–587
Kitahara T, Sato N, Ohya Y et al (1995) The inhibitory effect of ω-hydroxy acids in royal jelly extract on sebaceous gland lipogenesis. J Dermatol Sci 10:75–75
Yatsunami K, Echigo T (1985) Antibacterial action of royal jelly. Bull Fac Agric Tamagawa Univ 25:13–22
Amro NA, Kotra LP, Wadu-Mesthrige K et al (2000) High-resolution atomic force microscopy studies of the Escherichia coli outer membrane: structural basis for permeability. Langmuir 16:2789–2796
Youqing Xu, Shaonan Pang, Zhaokun Ding et al (2011) Effect of gene expression and mechanism on polyunsaturated fatty acid. Feed Industry 32(2):56–60
Jingyao Xu, Baoqin Ren (2000) Study and utilization of royaljelly acid in royal jelly. Chinese medicine science and Technology Press, Beijing, pp 109–113
Acknowledgments
This work was supported by grants from the National Natural Science Foundation of China (No: 31171727 and 31201281).
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Yang, X., Wang, T., Wang, R. (2014). Antibacterial Activity and Mechanism of Action of 10-HDA Against Escherichia coli . In: Zhang, TC., Ouyang, P., Kaplan, S., Skarnes, B. (eds) Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012). Lecture Notes in Electrical Engineering, vol 249. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-37916-1_60
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DOI: https://doi.org/10.1007/978-3-642-37916-1_60
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