Abstract
Administration of the non-competitive N-methyl-D-aspartate (NMDA) receptor antagonist phencyclidine (PCP) to rodents has been used as the gold standard preclinical model for schizophrenia. PCP treatment induces hyperlocomotion and stereotypic behaviour, which resemble the positive symptoms of schizophrenia. In addition, proteomic studies have identified changes in proteins associated with energy metabolism and neurotransmission which are typical hallmarks of the disease. This chapter presents a protocol for the generation of this model, behavioural assessment and preparation of key bio-samples to provide the raw materials for proteomic analyses.
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References
Allen RM, Young SJ (1978) Phencyclidine-induced psychosis. Am J Psychiatry 135:1081–1084
Sturgeon RD, Fessler RG, Meltzer HY (1979) Behavioral rating scales for assessing phencyclidine-induced locomotor activity, stereotyped behavior and ataxia in rats. Eur J Pharmacol 59:169–179
Nabeshima T, Yamaguchi K, Yamada K, Hiramatsu M, Furukawa H, Kameyama T (1983) Phencyclidine-induced stereotyped behaviors in rats following specific neurotoxin lesions of the striatum. Eur J Pharmacol 93:229–234
Javitt DC, Zukin SR (1991) Recent advances in the phencyclidine model of schizophrenia. Am J Psychiatry 148:1301–1308
Sams-Dodd F (1995) Distinct effects of D-amphetamine and phencyclidine on the social behaviour of rats. Behav Pharmacol 6:55–65
Egerton A, Reid L, McGregor S, Cochran SM, Morris BJ, Pratt JA (2008) Subchronic and chronic PCP treatment produces temporally distinct deficits in attentional set shifting and prepulse inhibition in rats. Psychopharmacology (Berl) 198:37–49
Adell A, Jimenez-Sanchez L, Lopez-Gil X, Romon T (2012) Is the acute NMDA receptor hypofunction a valid model of schizophrenia? Schizophr Bull 38:9–14
Anastasio NC, Xia Y, O’Connor ZR, Johnson KM (2009) Differential role of N-methyl-D-aspartate receptor subunits 2A and 2B in mediating phencyclidine-induced perinatal neuronal apoptosis and behavioral deficits. Neuroscience 163:1181–1191
Jenkins TA, Harte MK, Reynolds GP (2010) Effect of subchronic phencyclidine administration on sucrose preference and hippocampal parvalbumin immunoreactivity in the rat. Neurosci Lett 471:144–157
Palsson E, Lowry J, Klamer D (2010) Information processing deficits and nitric oxide signalling in the phencyclidine model of schizophrenia. Psychopharmacology (Berl) 212:643–651
Ernst A, Ma D, Garcia-Perez I, Tsang TM, Kluge W, Schwarz E et al (2012) Molecular validation of the acute phencyclidine rat model for schizophrenia: identification of translational changes in energy metabolism and neurotransmission. J Proteome Res 11:3704–3714
Wesseling H, Chan MK, Tsang TM, Ernst A, Peters F, Guest PC et al (2013) A combined metabonomic and proteomic approach identifies frontal cortex changes in a chronic phencyclidine rat model in relation to human schizophrenia brain pathology. Neuropsychopharmacology 38:2532–2544
Palmowski P, Rogowska-Wrzesinska A, Williamson J, Beck HC, Mikkelsen JD, Hansen HH et al (2014) Acute phencyclidine treatment induces extensive and distinct protein phosphorylation in rat frontal cortex. J Proteome Res 13:1578–1592
Wesseling H, Want EJ, Guest PC, Rahmoune H, Holmes E, Bahn S (2015) Hippocampal proteomic and metabonomic abnormalities in neurotransmission, oxidative stress, and apoptotic pathways in a chronic phencyclidine rat model. J Proteome Res 14:3174–3187
Ma D, Chan MK, Lockstone HE, Pietsch SR, Jones DN, Cilia J et al (2009) Antipsychotic treatment alters protein expression associated with presynaptic function and nervous system development in rat frontal cortex. J Proteome Res 8:3284–3297
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Ma, D., Guest, P.C. (2017). Generation of the Acute Phencyclidine Rat Model for Proteomic Studies of Schizophrenia. In: Guest, P. (eds) Proteomic Methods in Neuropsychiatric Research. Advances in Experimental Medicine and Biology(), vol 974. Springer, Cham. https://doi.org/10.1007/978-3-319-52479-5_23
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DOI: https://doi.org/10.1007/978-3-319-52479-5_23
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