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Magnetic Activated Cell Sorting of Human Spermatozoa

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In Vitro Fertilization

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Abstract

Magnetic separation has been successfully applied to many aspects of both biomedical and biological research and also in clinical areas like cellular therapies for human autoimmune diseases, like rheumatoid arthritis, diabetes, multiple sclerosis, and systemic lupus erythematosus, and nucleic acid transfer as a transfection method to optimize conditions for virus-mediated gene delivery (therapy) by magnetofection. In the last decade, several studies have been carried out on the use of magnetic cell sorting in human reproduction, for decontamination of testicular cell suspensions in cancer patients and for elimination of apoptotic spermatozoa from human semen samples.

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Author information

Authors and Affiliations

  1. Obstetrics and Gynecology, Center for Assisted Reproduction, Akdeniz University, Faculty of Medicine, Antalya, Turkey

    Enver Kerem Dirican

Authors
  1. Enver Kerem Dirican
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Editor information

Editors and Affiliations

  1. Reproductive Biology Associates – Prelude, Atlanta, GA, USA

    Zsolt Peter Nagy

  2. Astra Fertility Group, Mississauga, ON, Canada

    Alex C. Varghese

  3. Cleveland Clinic, Cleveland, OH, USA

    Ashok Agarwal

Review Questions

Review Questions

  1. 1.

    What is the purpose of eliminating apoptotic spermatozoa prior to assisted reproduction?

  2. 2.

    Explain the depletion and enrichment techniques in magnetic cell sorting technologies.

  3. 3.

    What are the advantages of sperm selection in terms of improving assisted reproduction outcome?

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Dirican, E.K. (2019). Magnetic Activated Cell Sorting of Human Spermatozoa. In: Nagy, Z., Varghese, A., Agarwal, A. (eds) In Vitro Fertilization. Springer, Cham. https://doi.org/10.1007/978-3-319-43011-9_29

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  • DOI: https://doi.org/10.1007/978-3-319-43011-9_29

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  • Online ISBN: 978-3-319-43011-9

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