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Clinical outcome of magnetic activated cell sorting of non-apoptotic spermatozoa before density gradient centrifugation for assisted reproduction

  • ASSISTED REPRODUCTION
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Abstract

Purpose

Magnetic activated cell sorting (MACS) eliminates apoptotic spermatozoa based on the presence of externalized phosphatidylserine residues. We evaluated the outcome of male fertility treatment when intracytoplasmic sperm injection (ICSI) into human oocytes was performed with non-apoptotic MACS-selected spermatozoa.

Methods

196 couples were treated by ICSI following spermatozoa preparation by MACS (study group; 122 couples) or density gradient centrifugation (DGC) (control group; 74 couples). Fertilization, cleavage, pregnancy, and implantation rates were analyzed.

Results

The percentage of sperm with normal morphology after MACS selection was improved. Cleavage and pregnancy rates were higher, respectively, in the study group than in control. A slightly higher implantation rate was also observed in the study group.

Conclusions

MACS selection of human spermatozoa increased cleavage and pregnancy rates in oligoasthenozoospermic ART cases. This novel method for selecting non-apoptotic spermatozoa for ICSI is safe and reliable, and may improve the assisted reproduction outcome.

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Authors and Affiliations

  1. Embryology, MAYA Center for Assisted Reproductive Technologies, Ankara, Turkey

    Enver Kerem Dirican & Özge Ercan

  2. Obstetrics and Gynecology, MAYA Center for Assisted Reproductive Technologies, Ankara, Turkey

    Osman Denizhan Özgün & Adnan Kaya

  3. Obstetrics and Gynecology, Keçiören Education and Research Hospital, Ankara, Turkey

    Süleyman Akarsu

  4. Biochemistry, Keçiören Education and Research Hospital, Ankara, Turkey

    Kadir Okhan Akın

  5. Andrology, MAYA Center for Assisted Reproductive Technologies, Ankara, Turkey

    Mukaddes Uğurlu & Çağrı Çamsarı

  6. Public Health, MAYA Center for Assisted Reproductive Technologies, Ankara, Turkey

    Oya Kanyılmaz

  7. Urology, Keçiören Education and Research Hospital, Ankara, Turkey

    Ali Ünsal

Authors
  1. Enver Kerem Dirican
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  2. Osman Denizhan Özgün
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  3. Süleyman Akarsu
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  4. Kadir Okhan Akın
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  5. Özge Ercan
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  6. Mukaddes Uğurlu
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  7. Çağrı Çamsarı
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  8. Oya Kanyılmaz
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  9. Adnan Kaya
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  10. Ali Ünsal
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Correspondence to Enver Kerem Dirican.

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Capsule

MACS selection of human spermatozoa increased cleavage and pregnancy rates in oligoasthenozoospermic ART cases.

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Dirican, E.K., Özgün, O.D., Akarsu, S. et al. Clinical outcome of magnetic activated cell sorting of non-apoptotic spermatozoa before density gradient centrifugation for assisted reproduction. J Assist Reprod Genet 25, 375–381 (2008). https://doi.org/10.1007/s10815-008-9250-1

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  • DOI: https://doi.org/10.1007/s10815-008-9250-1

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