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Fundamentals of Expression in Mammalian Cells

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Advanced Technologies for Protein Complex Production and Characterization

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 896))

Abstract

Expression of proteins in mammalian cells is a key technology important for many functional studies on human and higher eukaryotic genes. Studies include the mapping of protein interactions, solving protein structure by crystallization and X-ray diffraction or solution phase NMR and the generation of antibodies to enable a range of studies to be performed including protein detection in vivo. In addition the production of therapeutic proteins and antibodies, now a multi billion dollar industry, has driven major advances in cell line engineering for the production of grams per liter of active proteins and antibodies. Here the key factors that need to be considered for successful expression in HEK293 and CHO cells are reviewed including host cells, expression vector design, transient transfection methods, stable cell line generation and cultivation conditions.

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Authors and Affiliations

  1. IONTAS Ltd, Babraham Research Campus, Babraham, Cambridge, CB22 3AT, UK

    Michael R. Dyson

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  1. Michael R. Dyson
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Correspondence to Michael R. Dyson .

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  1. Center for Biological Research, SpanishNationalResearchCouncil(CIB-CSIC), Madrid, Spain

    M. Cristina Vega

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Dyson, M.R. (2016). Fundamentals of Expression in Mammalian Cells. In: Vega, M. (eds) Advanced Technologies for Protein Complex Production and Characterization. Advances in Experimental Medicine and Biology, vol 896. Springer, Cham. https://doi.org/10.1007/978-3-319-27216-0_14

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