Abstract
Various techniques of orthotopic single lung transplantation in rats have been reported; however, their widespread use has been limited owing to the complexity of the procedure and the high mortality, respectively. We present a simplified and reproducible microsurgical lung transplantation model in rats with a high survival rate, rendering surgical assistance unnecessary.
Overall recovery rate from anesthesia and 1 day survival including the early period of establishment was 77.0 % (n = 97), however survival could be increased to and maintained at 100 % in later series. The overall long-term survival (28 days) of animals successfully recovered from anesthesia was 75.0 %. However, we found the long-term survival to be dependent on different animal suppliers and series with a variation between 61.5 % and 100 %. The most common postoperative transplantation related cause of death was pleural effusion. The lungs showed excellent perfusion and ventilation immediately upon transplantation. Blood gas samples drawn from the left pulmonary vein and histologic sections revealed excellent graft function. The donor operation lasted 21.6 ± 6.8 min, donor left lung dissection required 21.9 ± 4.8 min, and implantation required 100.9 ± 19.8 min with a tendency of improvement over the course of time.
This innovative method of orthotopic left single lung transplantation can be safely performed by one experienced surgeon and is associated with excellent results and a high degree of reproducibility. The model presented is a valuable tool for research in ischemia reperfusion injury, acute lung edema, vascular inflammation and chronic rejection.
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© 2015 Springer International Publishing Switzerland
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Habertheuer, A., Wiedemann, D., Laufer, G., Kocher, A., Andreas, M. (2015). Orthotopic Experimental Lung Transplantation. In: Girman, P., Kriz, J., Balaz, P. (eds) Rat Experimental Transplantation Surgery. Springer, Cham. https://doi.org/10.1007/978-3-319-14559-4_10
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DOI: https://doi.org/10.1007/978-3-319-14559-4_10
Publisher Name: Springer, Cham
Print ISBN: 978-3-319-14558-7
Online ISBN: 978-3-319-14559-4
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