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The Plus-Maze Discriminative Avoidance Task: An Ethical Rodent Model for Concomitant Evaluation of Learning, Memory, Anxiety, Motor Activity and Their Interactions

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Rodent Model as Tools in Ethical Biomedical Research

Abstract

The elevated plus-maze consists of two open, elevated arms running along a north-south axis and two arms enclosed by walls running east-west. It combines elements of unfamiliarity, openness and elevation (see Chap. 16). Since its introduction by Handley and Mithani (1984) and experimental validation for rats (Pellow et al. 1985) and mice (Lister 1987), it has been most extensively used to asses anxiolytic and anxiogenic effects of drugs in several hundred studies. Standard anxiolytic and anxiogenic drugs increase and decrease, respectively, both the percentage of time that rats (or mice) spend in the open arms and the percentage of entries that they make into the open arms. However, due to its widespread appeal, the elevated plus-maze has also been used to measure levels of spontaneous anxiety behavior (Goto et al. 1993), to investigate anxiety-induced antinociception (Lee and Rodgers 1990; Frussa-Filho et al. 1992; Conceição et al. 1992; Rodgers et al. 1992) and to evaluate memory. Regarding this latter use, Itoh et al. (1990) proposed that the retesting-induced decrease in the time taken by the animal to move from an open arm to an enclosed arm in the elevated plus-maze could be a measure of memory. This suggestion has been corroborated and extended by others (Frussa-Filho et al. 1991; Graeff et al. 1993; Conceição et al. 1994). For example, Graeff et al. (1993) showed that the retesting-induced increase in the time taken by the animal to move from an enclosed arm to an open arm could be used as an inhibitory avoidance task to measure memory.

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Notes

  1. 1.

    The authors would like to acknowledge the fundamental contribution of Dr. Regina Helena da Silva to the development and validation of the PM-DAT.

  2. 2.

    The noise is produced by the horizontal displacement of a piece of metal against a piece of wood.

References

  • Abílio VC, Freitas FM, Dolnikoff MS, Castrucci AM, Frussa-Filho R. Effects of continuous exposure to light on behavioral dopaminergic supersensitivity. Biol Psychiatry. 1999;45:1622–9.

    Article  PubMed  Google Scholar 

  • Agnati LF, Fuxe K, Benfenati F, Batistini N, Zini I, Toffano G. Chronic ganglioside treatment counteracts the biochemical signs of dopamine receptor supersensitivity induced by chronic haloperidol treatment. Neurosci Lett. 1983;40:293–7.

    Article  CAS  PubMed  Google Scholar 

  • Aporti F, Borsato R, Calderini G, Rubini R, Toffano G, Zanotti A, et al. Age-dependent spontaneous EEG bursts in rats: effects of brain phosphatidylserine. Neurobiol Aging. 1986;7:115–20.

    Article  CAS  PubMed  Google Scholar 

  • Bellot RG, Camarini R, Vital MA, Palermo-Neto J, Leyton V, Frussa-Filho R. Monosialoganglioside attenuates the excitatory and behavioural sensitization effects of ethanol. Eur J Pharmacol. 1996;313(3):175–9.

    Article  CAS  PubMed  Google Scholar 

  • Beracochea D. Anterograde and retrograde effects of benzodiazepines on memory. Sci World J. 2006;6:1460–5.

    Article  CAS  Google Scholar 

  • Bonnet MH, Arand DL. We are chronically sleep deprived. Sleep. 1995;18:908–11.

    CAS  PubMed  Google Scholar 

  • Bonnet MH, Arand DL. Clinical effects of sleep fragmentation versus sleep deprivation. Sleep Med Rev. 2003;7:297–310.

    Article  PubMed  Google Scholar 

  • Bouyer JJ, Deminiere W, Mayo W, Le Moal M. Inter-individual differences in the effects of acute stress on the sleep-wakefulness cycle in the rat. Neurosci Lett. 1997;225:193–6.

    Article  CAS  PubMed  Google Scholar 

  • Bruins Slot LA, Colpaert FC. Opiate states of memory: receptor mechanisms. J Neurosci. 1999;19:10520–9.

    CAS  PubMed  Google Scholar 

  • Bruins Slot LA, Koek W, Colpaert FC. Ethanol state dependence involving a lever press response requirement in rats. Behav Pharmacol. 1999;10:229–33.

    Article  CAS  PubMed  Google Scholar 

  • Bueno OF, Lobo LL, Oliveira MG, Gugliano EB, Pomarico AC, Tufik S. Dissociated paradoxical sleep deprivation effects on inhibitory avoidance and conditioned fear. Physiol Behav. 1994;56:775–9.

    Article  CAS  PubMed  Google Scholar 

  • Calzavara MB, Lopez GB, Abílio VC, Silva RH, Frussa-Filho R. Role of anxiety levels in memory performance of spontaneously hypertensive rats. Behav Pharmacol. 2004;15:545–53.

    Article  CAS  PubMed  Google Scholar 

  • Carvalho RC, Silva RH, Abílio VC, Barbosa PN, Frussa-Filho R. Antidyskinetic effects of risperidone on animal models of tardive dyskinesia in mice. Brain Res Bull. 2003;60:115–24.

    Article  CAS  PubMed  Google Scholar 

  • Carvalho RC, Patti CL, Takatsu-Coleman AL, Kameda SR, Souza CF, Garcez-do-Carmo L, et al. Effects of reserpine on the plus-maze discriminative avoidance task: dissociation between memory and motor impairments. Brain Res. 2006;1122:179–83.

    Article  CAS  PubMed  Google Scholar 

  • Castro JP, Frussa-Filho R, Fukushiro DF, Chinen CC, Abílio VC, Silva RH. Effects of long-term continuous exposure to light on memory and anxiety in mice. Physiol Behav. 2005;86:218–23.

    Article  CAS  PubMed  Google Scholar 

  • Castro JP, Frussa-Filho R, Fukushiro DF, Silva RH, Medrano WA, Ribeiro RA, et al. Effects of baclofen on reserpine-induced vacuous chewing movements in mice. Brain Res Bull. 2006;68:436–41.

    Article  CAS  PubMed  Google Scholar 

  • Claro FT, Silva RH, Frussa-Filho R. Bovine brain phosphatidylserine attenuates scopolamine-induced amnesia. Physiol Behav. 1999;67:551–4.

    Article  CAS  PubMed  Google Scholar 

  • Cochran JC, Thorne DR, Penetar DM, Newhouse PA. Decoupling motor memory strategies: effects of sleep deprivation and amphetamine. Int J Neurosci. 1994;74:45–54.

    Article  CAS  PubMed  Google Scholar 

  • Cohen C, Perrault G, Sanger DJ. Evidence for the involvement of dopamine receptors in ethanol-induced hyperactivity in mice. Neuropharmacology. 1997;36:1099–108.

    Article  CAS  PubMed  Google Scholar 

  • Colpaert FC. Pharmacological characteristics of tremor, rigidity and hypokinesia induced by reserpine in rats. Neuropharmacology. 1987;26:1431–40.

    Article  CAS  PubMed  Google Scholar 

  • Colpaert FC. Amnestic trace locked into the benzodiazepine state of memory. Psychopharmacology (Berl). 1990;102:28–36.

    Article  CAS  Google Scholar 

  • Colpaert FC. System theory of pain and of opiate analgesia: no tolerance to opiates. Pharmacol Rev. 1996;47:605–29.

    Google Scholar 

  • Colpaert FC, Koek W, Bruins Slot LA. Evidence that amnesic state govern normal and disordered memory. Behav Pharmacol. 2001;12:575–89.

    Article  CAS  PubMed  Google Scholar 

  • Conceição IM, Maiolini Jr M, Mattia MA, Vital MA, Santos BR, Smaili S, et al. Anxiety-induced antinociception in the mouse. Braz J Med Biol Res. 1992;25:831–4.

    PubMed  Google Scholar 

  • Conceição IM, Maioline Jr M, Mattia NF, Chang YH, Smaili S, Frussa-Filho R. Effect of withdrawal from long-term nifedipine administration on open-field habituation in the rat. Braz J Med Biol Res. 1994;27:1363–7.

    PubMed  Google Scholar 

  • Cooper JA, Sagar HJ, Jordan N, Harvey NS, Sullivan E. Cognitive impairment in early, untreated Parkinson’s disease and its relationship to motor disability. Brain. 1991;14:2095–122.

    Article  Google Scholar 

  • Crook T, Petrie W, Wells C, Massari DC. Effects of phosphatidylserine in Alzheimer’s disease. Psychopharmacol Bull. 1992;28:61–6.

    CAS  PubMed  Google Scholar 

  • Dinges DF, Pack F, Williams K, Gillen KA, Powell JW, Ott GE, et al. Cumulative sleepiness, mood disturbance, and psychomotor vigilance performance decrements during a week of sleep restricted to 4–5 hours per night. Sleep. 1997;20:267–77.

    CAS  PubMed  Google Scholar 

  • Fluck E, File SE, Springett J, Kopelman MD, Rees J, Orgill J. Does the sedation resulting from sleep deprivation and lorazepam cause similar cognitive deficits? Pharmacol Biochem Behav. 1998;59:909–15.

    Article  CAS  PubMed  Google Scholar 

  • Frussa-Filho R, Otoboni JR, Uema FT, Sá-Rocha LC. Evaluation of memory and anxiety in rats observed in the elevated plus-maze: effects of age and isolation. Braz J Med Biol Res. 1991;24:725–8.

    CAS  PubMed  Google Scholar 

  • Frussa-Filho R, Otoboni JR, Gianotti AD, Amaral ACS, Conceição IM. Effects of age on antinociceptive effects of elevated plus-maze exposure. Braz J Med Biol Res. 1992;25:827–9.

    CAS  PubMed  Google Scholar 

  • Frussa-Filho R, Barbosa-Júnior H, Silva RH, Da Cunha C, Mello CF. Naltrexone potentiates the anxiolytic effects of chlordiazepoxide in rats exposed to novel environments. Psychopharmacology (Berl). 1999;147:168–73.

    Article  CAS  Google Scholar 

  • Goldstone S, Nurnberg HG, Lhamon WT. Effects of trifluoperazine, chlorpromazine, and haloperidol upon temporal information processing by schizophrenic patients. Psychopharmacology (Berl). 1979;65:119–24.

    Article  CAS  Google Scholar 

  • Goto SH, Conceição IM, Ribeiro RA, Frussa-Filho R. Comparison of anxiety measured in the elevated plus-maze, open-field and social interaction tests between spontaneously hypertensive rats and Wistar EPM 1 rats. Braz J Med Biol Res. 1993;26:965–9.

    CAS  PubMed  Google Scholar 

  • Graeff FG, Vianna MB, Tomaz C. The elevated T-maze, a new experimental model of anxiety and memory: effect of diazepam. Braz J Med Biol Res. 1993;26:67–70.

    CAS  PubMed  Google Scholar 

  • Handley SL, Mitani S. Effects of alfa-adrenoceptor agonists and antagonists in a maze exploration model of ‘fear’-motivated behavior. Naunyn Schmiedebergs Arch Pharmacol. 1984;327:1–5.

    Article  CAS  PubMed  Google Scholar 

  • Hanks GW, O’Neill WM, Simpson P, Wesnes K. The cognitive and psychomotor effects of opioid analgesics. II. A randomized controlled trial of single doses of morphine, lorazepam and placebo in healthy subjects. Eur J Clin Pharmacol. 1995;48:455–60.

    Article  CAS  PubMed  Google Scholar 

  • Hernández OH, Vogel-Sprott M, Ke-Aznar VI. Alcohol impairs the cognitive component of reaction time to an omitted stimulus: a replication and an extension. J Stud Alcohol Drugs. 2007;68:276–81.

    Article  PubMed  Google Scholar 

  • Isomae K, Morimoto S, Hasegawa H, Morita K, Kamei J. Effects of T-82, a novel acetylcholinesterase inhibitor, on impaired learning and memory in passive avoidance task in rats. Eur J Pharmacol. 2003;465:97–103.

    Article  CAS  PubMed  Google Scholar 

  • Itoh J, Nabeshima T, Kameyama T. Utility of an elevated plus-maze for the evaluation of memory in mice: effects of nootropics, scopolamine and electroconvulsive shock. Psychopharmacology (Berl). 1990;101:27–33.

    Article  CAS  Google Scholar 

  • Izquierdo I, Perry ML, Dias RD, Souza DO, Elizabetsky E, Carrasco MA, et al. Endogenous opioids memory modulation and state dependency. In: Martinez JL, Jensen RA, Messing RB, Rigter H, McGaugh JL, editors. Endogenous peptides and learning and memory process. New York: Academic; 1981. p. 269–90.

    Google Scholar 

  • Jackson A, Koek W, Colpaert FC. NMDA antagonists make learning and recall state-dependent. Behav Pharmacol. 1992;3:415–21.

    Article  CAS  PubMed  Google Scholar 

  • Kameda SR, Frussa-Filho R, Carvalho RC, Takatsu-Coleman AL, Ricardo VP, Patti CL, et al. Dissociation of the effects of ethanol on memory, anxiety, and motor behavior in mice tested in the plus-maze discriminative avoidance task. Psychopharmacology (Berl). 2007;192:39–48.

    Article  CAS  Google Scholar 

  • Karni A, Tanne D, Rubenstein BS, Askenasy JJ, Sagi D. Dependence on REM sleep of overnight improvement of a perceptual skill. Science. 1994;265:679–82.

    Article  CAS  PubMed  Google Scholar 

  • Lee C, Rodgers RJ. Antinociceptive effects of elevated plus-maze exposure: influence of opiate receptor manipulations. Psychopharmacology (Berl). 1990;102:507–13.

    Article  CAS  Google Scholar 

  • Levin ED, Wilson W, Rose JE, McEvoy J. Nicotine-haloperidol interactions and cognitive performance in schizophrenics. Neuropsychopharmacology. 1996;15:429–36.

    Article  CAS  PubMed  Google Scholar 

  • Lister RG. The use of a plus-maze to measure anxiety in the mouse. Psychopharmacology (Berl). 1987;92:180–5.

    CAS  Google Scholar 

  • Martinez Jr JL, Schulteis G, Janak PH, Weinberger SB. Behavioral assessment of forgetting in aged rodents and its relationship to peripheral sympathetic function. Neurobiol Aging. 1988;9:697–708.

    Article  PubMed  Google Scholar 

  • Mednick SC, Nakayama K, Cantero JL, Atienza M, Levin AA, Pathak N, Stickgold R. The restorative effect of naps on perceptual deterioration. Nat Neurosci. 2002;5:677–81.

    CAS  PubMed  Google Scholar 

  • Miceli G, Caltagirone C, Gainotti G. Gangliosides in the treatment of mental deterioration. A double-blind comparison with placebo. Acta Psychiatr Scand. 1997;55:102–10.

    Article  Google Scholar 

  • Mizumori SJ, Sakai DH, Rosenzweig MR, Bennett EL, Wittreich P. Investigations into the neuropharmacological basis of temporal stages of memory formation in mice trained in an active avoidance task. Behav Brain Res. 1987;23:239–50.

    Article  CAS  PubMed  Google Scholar 

  • Nunzi MG, Milan F, Guidolin D, Zanotti A, Toffano G. Therapeutic properties of phosphatidylserine in the aging brain. In: Hanin I, Pepeu G, editors. Phospholipids. New York: Plenum Press; 1990. p. 213–8.

    Chapter  Google Scholar 

  • Owen AM, James M, Leigh PN, Summers BA, Marsden CD, Quinn NP, et al. Fronto-striatal cognitive deficits at different stages of Parkinson’s disease. Brain. 1992;115:1727–51.

    Article  PubMed  Google Scholar 

  • Patti CL, Kameda SR, Carvalho RC, Takatsu-Coleman AL, Lopez GB, Niigaki ST, et al. Effects of morphine on the plus-maze discriminative avoidance task: role of state-dependent learning. Psychopharmacology (Berl). 2006;184:1–12.

    Article  CAS  Google Scholar 

  • Pellow S, Chopin P, File SE, Briley M. Validation of open: closed arm entries in an elevated plus-maze as a measure of anxiety in the rat. J Neurosci Methods. 1985;14:149–67.

    Article  CAS  PubMed  Google Scholar 

  • Pierce RC, Kalivas PW. A circuitry model of the expression of behavioral sensitization to amphetamine-like psychostimulants. Brain Res Brain Res Rev. 1997;25:192–216.

    Article  CAS  PubMed  Google Scholar 

  • Rahmann H. Brain gangliosides: neuromodulators for synaptic transmission and memory formation. In: Matthies H, editor. Advances in the biosciences. Oxford: Pergamon Press; 1986. p. 235–45.

    Google Scholar 

  • Ramos A, Kangerski AL, Basso PF, Da Silva Santos JE, Assreuy J, Vendruscolo LF, et al. Evaluation of Lewis and SHR rat strains as a genetic model for the study of anxiety and pain. Behav Brain Res. 2002;129:113–23.

    Article  PubMed  Google Scholar 

  • Rapeli P, Kivisaari R, Kähkönen S, Puuskari V, Autti T, Kalska H. Do individuals with former amphetamine dependence have cognitive deficits? Nord J Psychiatry. 2005;59:293–7.

    PubMed  Google Scholar 

  • Rapport MM. Introduction to the biochemistry of gangliosides. In: Rapport MM, Gorio A, editors. Gangliosides in neurobiological and neuromuscular function, development and repair. New York: Raven; 1981. p. 15–9.

    Google Scholar 

  • Rodgers RJ, Lee C, Shepherd JK. Effects of diazepam on behavioural and antinociceptive responses to elevated plus-maze in male mice depend upon treatment regimen and prior maze experience. Psychopharmacology (Berl). 1992;106:102–10.

    Article  CAS  Google Scholar 

  • Rosenzweig MR, Bennett EL, Colombo PJ, Lee DW, Serrano PA. Short-term, intermediate-term, and long-term memories. Behav Brain Res. 1993;5:193–8.

    Article  Google Scholar 

  • Schreiber S, Kampf-Sherf O, Gorfine M, Kelly D, Oppenheim Y, Lerer B. An open trial of plant-source derived phosphatydilserine for treatment of age-related cognitive decline. Isr J Psychiatry Relat Sci. 2000;37:302–7.

    CAS  PubMed  Google Scholar 

  • Seifert W, Wierasko A, Terlau H, Hollmann M. Gangliosides and neuronal plasticity in the hippocampus. NATO ASI Ser. 1987;7:523–8.

    CAS  Google Scholar 

  • Siegel JM. The REM, sleep-memory consolidation hypothesis. Science. 2001;294:1058–63.

    Article  CAS  PubMed  Google Scholar 

  • Silva RH, Frussa-Filho R. The plus-maze discriminative avoidance task: a new model to study memory-anxiety interactions. Effects of chlordiazepoxide and caffeine. J Neurosci Methods. 2000;102:117–25.

    Article  CAS  PubMed  Google Scholar 

  • Silva RH, Frussa-Filho R. Naltrexone potentiates both amnestic and anxiolytic effects of chlordiazepoxide in mice. Life Sci. 2002;72:721–30.

    Article  CAS  PubMed  Google Scholar 

  • Silva RH, Bellot RG, Vital MABF, Frussa-Filho R. Effects of long-term ganglioside GM1 administration on a new discriminative avoidance test in normal adult mice. Psychopharmacology (Berl). 1997;129:322–8.

    CAS  Google Scholar 

  • Silva RH, Felicio LF, Frussa-Filho R. Ganglioside GM1 attenuates scopolamine-induced amnesia in rats and mice. Psychopharmacology (Berl). 1999;141:111–7.

    Article  CAS  Google Scholar 

  • Silva RH, Abílio VC, Torres-Leite D, Bergamo M, Chinen CC, Claro FT, et al. Concomitant development of oral dyskinesia and memory deficits in reserpine-treated male and female mice. Behav Brain Res. 2002a;132:171–7.

    Article  CAS  PubMed  Google Scholar 

  • Silva RH, Kameda SR, Carvalho RC, Rigo GS, Costa KL, Taricano ID, et al. Effects of amphetamine on the plus-maze discriminative avoidance task in mice. Psychopharmacology (Berl). 2002b;160:9–18.

    Article  CAS  Google Scholar 

  • Silva RH, Abílio VC, Takatsu AL, Kameda SR, Grassl C, Chehin AB, et al. Role of hippocampal oxidative stress in memory deficits induced by sleep deprivation in mice. Neuropharmacology. 2004a;46:895–903.

    Article  CAS  PubMed  Google Scholar 

  • Silva RH, Chehin AB, Kameda SR, Takatsu-Coleman AL, Abílio VC, Tufik S, et al. Effects of pre- or post-training paradoxical sleep deprivation on two animal models of learning and memory in mice. Neurobiol Learn Mem. 2004b;82:90–8.

    Article  CAS  PubMed  Google Scholar 

  • Smith C, Kelly G. Paradoxical sleep deprivation applied two days after end of training retards learning. Physiol Behav. 1998;43:213–6.

    Article  Google Scholar 

  • Spanagel R. Modulation of drug-induced sensitization processes by endogenous opioid systems. Behav Brain Res. 1995;70:37–49.

    Article  CAS  PubMed  Google Scholar 

  • Stip E. Cognition, schizophrenia and the effect of antipsychotics. Encéphale. 2006;32:341–50.

    Article  CAS  PubMed  Google Scholar 

  • Suchecki D, Duarte-Palma B, Tufik S. Sleep rebound in animals deprived of paradoxical sleep by the modified multiple platform method. Brain Res. 2000;875:14–22.

    Article  CAS  PubMed  Google Scholar 

  • Sutton MA, Masters SE, Bagnall MW, Carew TJ. Molecular mechanisms underlying a unique intermediate phase of memory in aplysia. Neuron. 2001;31:143–54.

    Article  CAS  PubMed  Google Scholar 

  • Tettamanti G, Sonnino S, Ghiondi R, Masserini M, Venerando B. Chemical and functional properties of gangliosides. Their possible implication in the membrane mediated transfer of information. In: Degiorgio V, Corti M, editors. Physics and amphiphiles: micelles, vesicles and microemulsions. Amsterdam: North Holland Physics; 1985. p. 607–36. XC Corso Soc Ital Fisica Bologna.

    Google Scholar 

  • Tipper CM, Cairo TA, Woodward TS, Phillips AG, Liddle PF, Ngan ET. Processing efficiency of a verbal working memory system is modulated by amphetamine: an fMRI investigation. Psychopharmacology (Berl). 2005;180:634–43.

    Article  CAS  Google Scholar 

  • Toffano G, Agnati LF, Fuxe KG. The effect of the ganglioside GM1 on neuronal plasticity. Int J Dev Neurosci. 1986;4:97–100.

    Article  CAS  PubMed  Google Scholar 

  • Walker MP, Stickgold R. Sleep-dependent learning and memory consolidation. Neuron. 2004;44:121–33.

    Article  CAS  PubMed  Google Scholar 

  • Youngblood BD, Zhou J, Smagin GN, Ryan DH, Harris RB. Sleep deprivation by the “flower pot” technique and spatial reference memory. Physiol Behav. 1997;61:249–56.

    Article  CAS  PubMed  Google Scholar 

  • Zanotti A, Valzelli L, Toffano G. Reversal of scopolamine-induced amnesia by phosphatidylserine in rats. Psychopharmacology (Berl). 1986;90:274–5.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Pharmacology, Universidade Federal de São Paulo (UNIFESP), São Paulo, São Paulo, Brazil

    Roberto Frussa-Filho, Luciana Takahashi Carvalho Ribeiro Ph.D., Sonia Regina Kameda & Rita de Cassia Carvalho

  2. Instituto de Genética e Erros Inatos do Metabolismo, Universidade Federal de São Paulo (UNIFESP), São Paulo, São Paulo, Brazil

    Camilla de Lima Patti Ph.D.

  3. Department of Pharmacology, Universidade Federal de São Paulo (UNIFESP), Rua Botucatu, 862, Ed. Leal Prado, 1º andar, São Paulo,, São Paulo, 04023062, Brazil

    Daniela Fukue Fukushiro Ph.D.

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  1. Roberto Frussa-Filho
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  1. Department of Psychobiology, Universidade Federal de São Paulo (UNIFESP), São Paulo, São Paulo, Brazil

    Monica Levy Andersen

  2. Department of Psychobiology, Universidade Federal de São Paulo (UNIFESP), São Paulo, São Paulo, Brazil

    Sergio Tufik

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Frussa-Filho, R., de Lima Patti, C., Fukushiro, D.F., Ribeiro, L.T.C., Kameda, S.R., de Cassia Carvalho, R. (2016). The Plus-Maze Discriminative Avoidance Task: An Ethical Rodent Model for Concomitant Evaluation of Learning, Memory, Anxiety, Motor Activity and Their Interactions. In: Andersen, M., Tufik, S. (eds) Rodent Model as Tools in Ethical Biomedical Research. Springer, Cham. https://doi.org/10.1007/978-3-319-11578-8_19

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