Skip to main content

Co-inoculation of Legumes with Azospirillum and Symbiotic Rhizobia

  • Chapter
Handbook for Azospirillum

Abstract

Azospirilla are generally regarded as rhizosphere bacteria and colonize the root surface and only a few strains are able to infect plants. The stimulatory effect exerted by Azospirillum has been attributed to several mechanisms but under certain environmental and soil conditions, Azospirillum can positively influence plant growth, crop yields, and N-content of the legume. Most Azospirillum–rhizobacteria co-inoculation studies have focused on the final effects on plant growth and nodulation parameters; in contrast, few data are available on the simultaneous effect of double inoculation and stressful conditions on the early signalling exchange between the symbiotic partners. Co-inoculation of legume with rhizobia plus Azospirillum exerted changes on the concentration, content, and/or distribution of several mineral nutrients in roots and/or shoots of plants, change in flavonoids, and can help to tolerate stress. This chapter summarized some effects that occur when legumes are co-inoculated with Azospirillum.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 189.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 249.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 249.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  • Bashan Y, Harrison K, Whitmoyer R (1990) Enhanced growth of wheat and soybean plants inoculated with Azospirillum brasilense is not necessarily due to general enhancement of mineral uptake. Appl Environ Microbiol 56:769–775

    PubMed Central  CAS  PubMed  Google Scholar 

  • Bashan Y, Holguin G, de-Bashan LE (2004) Azospirillum-plant relationships: physiological, molecular, agricultural, and environmental advances (1997–2003). Can J Microbiol 50:521–577

    Article  CAS  PubMed  Google Scholar 

  • Burdman S, Kigel J, Okon Y (1997) Effects of Azospirillum brasilense on nodulation and growth of common bean (Phaseolus vulgaris L.). Soil Biol Biochem 29:923–929

    Article  CAS  Google Scholar 

  • Burdman S, Jurkevitch E, Schwartsburd B, Hampel M, Okon Y (1998) Aggregation in Azospirillum brasilense: effects of chemical and physical factors and involvement of extracellular components. Microbiology 144:1989–1999

    Article  CAS  PubMed  Google Scholar 

  • Cassan F, García de Salomone I (eds) (2008) Azospirillum sp.: cell physiology, plant interactions and agronomic research in Argentina. Asociación Argentina de Microbiología, Buenos Aires, pp 141–151

    Google Scholar 

  • Dardanelli MS, Fernández FJ, Espuny MR, Rodríguez MA, Soria ME, Gil Serrano AM, Okon Y, Megías M (2008) Effect of Azospirillum brasilense coinoculated with Rhizobium on Phaseolus vulgaris flavonoids and Nod factor production under salt stress. Soil Biol Biochem 40:2713–2721

    Article  CAS  Google Scholar 

  • Dardanelli MS, Carletti SM, Paulucci NS, Medeot DB, Rodriguez Cáceres EA, Vita FA, Bueno MA, Fumero MV, Garcia MB (2010) Benefits of plant growth promoting rhizobacteria (PGPR) and rhizobia in agriculture. In: Maheshwari DK (ed) Bacteria and plant health. Springer, Berlin, pp 1–20

    Chapter  Google Scholar 

  • Döbereiner J, Pedrosa FO (1987) Nitrogen-fixing bacteria in non-leguminous crop plants. Springer, Madison

    Google Scholar 

  • Fallik E, Okon Y, Fischer M (1988) The effect of Azospirillum brasilense inoculation on metabolic enzyme activity in maize root seedlings. Symbiosis 6:17–28

    Google Scholar 

  • Helman Y, Burdman S, Okon Y (2011) Plant growth promotion by rhizosphere bacteria through direct effects. In: Rosenberg E, Gophna U (eds) Beneficial microorganisms in multicellular life forms. Springer, Berlin, pp 89–103

    Google Scholar 

  • Hungria M, Nogueira MA, Araujo RS (2013) Co-inoculation of soybeans and common beans with rhizobia and azospirilla: strategies to improve sustainability. Biol Fertil Soils 49:791–801

    Article  Google Scholar 

  • Michiels K, Croes CL, Vanderleyden J (1991) Two different modes of attachment of Azospirillum brasilense Sp7 to wheat roots. J Gen Microbiol 137:2241–2246

    Article  CAS  Google Scholar 

  • Okon Y, Kapulnik Y (1986) Development and function of Azospirillum inoculated roots. Plant Soil 90:3–16

    Article  CAS  Google Scholar 

  • Okon Y, Labandera-González C (1994) Agronomic applications of Azospirillum: an evaluation of 20 years’ worldwide field inoculation. Soil Biol Biochem 26:1591–1601

    Article  CAS  Google Scholar 

  • Pacovsky RS, Paul EA, Bethlenfalvay GJ (1985) Nutrition of sorghum plants with nitrogen or inoculated with Azospirillum brasilense. Plant Soil 85:145–148

    Article  CAS  Google Scholar 

  • Plazinski J, Rolfe BG (1985) Influence of Azospirillum strains on the nodulation of clovers by Rhizobium strains. Appl Environ Microbiol 49:984–989

    PubMed Central  CAS  PubMed  Google Scholar 

  • Remans R, Beebe S, Blair M, Manrique G, Tovar E, Rao I, Croonenborghs A, Torres-Gutierrez R, El-Howeity M, Michiels J, Vanderleyden J (2008a) Physiological and genetic analysis of root responsiveness to auxin-producing plant growth-promoting bacteria in common bean (Phaseolus vulgaris L.). Plant Soil 302:149–161

    Article  CAS  Google Scholar 

  • Remans R, Ramaekers L, Schelkens S, Hernandez G, Garcia A, Reyes JL, Mendez N, Toscano V, MullingM GL, Vanderleyden J (2008b) Effect of RhizobiumAzospirillum coinoculation on nitrogen fixation and yield of two contrasting Phaseolus vulgaris L. genotypes cultivated across different environments in Cuba. Plant Soil 312:25–37

    Article  CAS  Google Scholar 

  • Rodelas B, González-López J, Martínez-Toledo MV, Pozo C, Salmerón V (1999) Influence of Rhizobium/Azotobacter and Rhizobium/Azospirillum combined inoculation on mineral composition of faba bean (Vicia faba L.). Biol Fertil Soils 29:165–169

    Article  CAS  Google Scholar 

  • Rodríguez-Navarro DN, Dardanelli MS, Ruiz Sainz JE (2007) Attachment of bacteria to the roots of higher plants. FEMS Microbiol Lett 272:127–136

    Article  PubMed  Google Scholar 

  • Skvortsov IM, Ignatov VV (1998) Extracellular polysaccharides and polysaccharide-containing biopolymers from Azospirillum species: properties and the possible role in interaction with plant roots. FEMS Microbiol Lett 165:223–229

    Article  CAS  PubMed  Google Scholar 

  • Smit G, Kijne J, Lugtenberg B (1986) Correlation between extracellular fibrils and attachment of Rhizobium leguminosarum to pea root hair tips. J Bacteriol 168:821–827

    PubMed Central  CAS  PubMed  Google Scholar 

  • Sozzani R, Busch W, Spalding EP, Benfey PN (2014) Advanced imaging techniques for the study of plant growth and development. Trends Plant Sci 19:304–310

    Article  CAS  PubMed  Google Scholar 

  • Spaink HP, Aarts A, Stacey G, Bloemberg GV, Lugtenberg BJJ, Kennedy EP (1992) Detection and separation of Rhizobium and Bradyrhizobium Nod metabolites using thin-layer chromatography. Mol Plant-Microbe Interact 5:72–80

    Article  CAS  PubMed  Google Scholar 

  • Star L, Matan O, Dardanelli MS, Kapulnik Y, Burdman S, Okon Y (2012) Effect of Azospirillum brasilense strain Sp7 and mutants, on Vicia sativa spp. nigra-Rhizobium leguminosarum bv viciae symbiosis. Plant Soil 356:165–174

    Article  CAS  Google Scholar 

  • Steenhoudt O, Vanderleyden J (2000) Azospirillum a free-living nitrogen-fixing bacterium closely associated with grasses: genetic, biochemical and ecological aspects. FEMS Microbiol Rev 24:487–506

    Article  CAS  PubMed  Google Scholar 

  • Volpin H, Kapulnik Y (1994) Interaction of Azospirillum with beneficial soil microorganisms. In: Okon Y (ed) Azospirillum/plant associations. CRC Press, Boca Raton, pp 111–118

    Google Scholar 

  • Yahalom E, Dovrat A, Okon Y, Czosnek H (1991) Effect of inoculation with Azospirillum brasilense strain Cd and Rhizobium on the morphology of burr medic (Medicago polymorpha L.). Isr J Bot 40:155–164

    Google Scholar 

  • Zhu GY, Dobbelaere S, Vanderleyden J (2002) Use of green fluorescent protein to visualized rice root colonization by Azospirillum irakense and A. brasilense. Funct Plant Biol 29:1279–1285

    Article  Google Scholar 

Download references

Acknowledgments

This research was partially supported by the CONICET PIP 112-201101-00309 and PID 2010 MINCyT Córdoba. JCV, LAG, and CK are fellows from CONICET and MSD and NSP are members of the research career of CONICET, Argentina.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Marta Susana Dardanelli .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2015 Springer International Publishing Switzerland

About this chapter

Cite this chapter

Vicario, J.C., Gallarato, L.A., Paulucci, N.S., Perrig, D.S., Bueno, M.Á., Dardanelli, M.S. (2015). Co-inoculation of Legumes with Azospirillum and Symbiotic Rhizobia. In: Cassán, F., Okon, Y., Creus, C. (eds) Handbook for Azospirillum. Springer, Cham. https://doi.org/10.1007/978-3-319-06542-7_22

Download citation

Publish with us

Policies and ethics