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Neurological Analyses: Focus on Gangliosides and Mass Spectrometry

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Advancements of Mass Spectrometry in Biomedical Research

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 806))

Abstract

Gangliosides, sialylated glycosphingolipids, are particularly enriched in mammalian central nervous system where their expression is cell type-specific and changes particularly during brain development, maturation, aging, and diseases. For this reason, gangliosides are important diagnostic markers for various brain ailments, including primary and secondary brain tumors and neurodegenerative diseases. Among all biochemical and biophysical methods employed so far for ganglioside analysis, mass spectrometry (MS) emerged as one of the most reliable due to the sensitivity, accuracy, and speed of analysis as well as the possibility to characterize in details the molecular structure of the identified biomarkers.

This chapter presents significant achievements of MS with either electrospray (ESI), chip-based ESI, or matrix-assisted laser desorption/ionization (MALDI) in the analysis of gangliosides in normal and diseased human brain. Specifically, the chapter assesses the MS contribution in determination of topospecificity, filogenetic, and brain development stage dependence of ganglioside composition and structure as well as in discovery of ganglioside markers in neurodegenerative/neurodevelopmental conditions, primary and secondary brain tumors. The highlighted accomplishments in characterization of novel structures associated to severe brain pathologies show that MS has real perspectives to become a routine method for early diagnosis and therapy based on this biomolecule class.

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Acknowledgments

This work was supported by the Romanian National Authority for Scientific Research, CNCS-UEFISCDI, projects PN-II-ID-PCE-2011-3-0047 PN-II-RU-2011-TE-0008 and PN-II-PCCA-2011-142 and by EU Commission, project FP7 Marie Curie-PIRSES-“MS-Life”-2010-269-256.

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Authors and Affiliations

  1. Faculty of Physics, West University of Timisoara, 4 Boulevard V. Parvan, Timisoara, Romania

    Alina D. Zamfir

  2. National Institute for Research and Development in Electrochemistry and Condensed Matter, 1 P. Andronescu Street, Timisoara, Romania

    Alina D. Zamfir

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Correspondence to Alina D. Zamfir .

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  1. Chemistry and Biomolecular Science Biochemistry and Proteomics Group, Clarkson University, Potsdam, New York, USA

    Alisa G. Woods

  2. Chemistry and Biomolecular Science Biochemistry and Proteomics Group, Clarkson University, Potsdam, New York, USA

    Costel C. Darie

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Zamfir, A.D. (2014). Neurological Analyses: Focus on Gangliosides and Mass Spectrometry. In: Woods, A., Darie, C. (eds) Advancements of Mass Spectrometry in Biomedical Research. Advances in Experimental Medicine and Biology, vol 806. Springer, Cham. https://doi.org/10.1007/978-3-319-06068-2_8

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