Abstract
Mass spectrometry (MS) is the core for advanced methods in proteomic experiments. When effectively used, proteomics may provide extensive information about proteins and their post-translational modifications, as well as their interaction partners. However, there are also many problems that one can encounter during a proteomic experiment, including, but not limited to sample preparation, sample fractionation, sample analysis, data analysis & interpretation, and biological significance. Here we discuss some of the problems that researchers should be aware of when performing a proteomic experiment.
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Acknowledgements
We would like to thank Ms. Laura Mulderig, Scott Nichols, and their colleagues (Waters Corporation) for their generous support in setting up the Proteomics Center at Clarkson University. CCD thanks Drs. Thomas A. Neubert (New York University), Belinda Willard (Cleveland Clinic), and Gregory Wolber & David Mclaughin (Eastman Kodak Company) for donation of a TofSpec2E MALDI-MS (each). C.C.D. thanks his advisors, Vlad Artenie, Wolfgang Haehnel, Paul M. Wassarman & Thomas A. Neubert for advice and support. This work was supported in part by the Keep a Breast Foundation (KEABF-375-35054), the Redcay Foundation (SUNY Plattsburgh), the Alexander von Humboldt Foundation, SciFund Challenge, the David A. Walsh fellowship, and by the U.S. Army research office (DURIP grant #W911NF-11-1-0304).
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Wetie, A.G.N., Shipp, D.A., Darie, C.C. (2014). Bottlenecks in Proteomics. In: Woods, A., Darie, C. (eds) Advancements of Mass Spectrometry in Biomedical Research. Advances in Experimental Medicine and Biology, vol 806. Springer, Cham. https://doi.org/10.1007/978-3-319-06068-2_29
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DOI: https://doi.org/10.1007/978-3-319-06068-2_29
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