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Guidelines on Cerebrospinal Fluid Analysis

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Cerebrospinal Fluid in Clinical Neurology

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Abstract

The examination of cerebrospinal fluid (CSF) is invaluable for the diagnosis of various neurological diseases. Whereas routine parameters such as white blood cell count, CSF/serum albumin quotient, CSF/serum glucose ratio, intrathecal fraction of immunoglobulins or cytological examination are used to obtain an integrative CSF report, there are also single disease-specific CSF biomarkers contributing to clinical diagnosis making. Furthermore, infectious agents can be detected in CSF either directly by microscopy and culture or indirectly by detection of antigens via polymerase chain reaction or of specific antibodies via serology. In this chapter, we discuss the diagnostic value of CSF referring to existing guidelines.

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References

  • Ahonen A, Myllyla VV, Hokkanen E (1979) Cerebrospinal fluid protein findings in various lower back pain syndromes. Acta Neurol Scand 60(2):93–99. PubMed PMID: 158934

    Article  CAS  PubMed  Google Scholar 

  • Aksamit AJ (2003) Cerebrospinal fluid 14-3-3 protein: variability of sporadic Creutzfeldt-Jakob disease, laboratory standards, and quantitation. Arch Neurol 60(6):803–804. PubMed PMID: 12810481

    Article  PubMed  Google Scholar 

  • Albert MS, DeKosky ST, Dickson D, Dubois B, Feldman HH, Fox NC et al (2011) The diagnosis of mild cognitive impairment due to Alzheimer’s disease: recommendations from the National Institute on Aging-Alzheimer’s Association workgroups on diagnostic guidelines for Alzheimer’s disease. Alzheimers Dement 7(3):270–279. PubMed PMID: 21514249. Pubmed Central PMCID: 3312027

    Article  PubMed Central  PubMed  Google Scholar 

  • Appleby BS, Appleby KK, Crain BJ, Onyike CU, Wallin MT, Rabins PV (2009) Characteristics of established and proposed sporadic Creutzfeldt-Jakob disease variants. Arch Neurol 66(2):208–215. PubMed PMID: 19204157

    Article  PubMed  Google Scholar 

  • Atarashi R, Satoh K, Sano K, Fuse T, Yamaguchi N, Ishibashi D et al (2011) Ultrasensitive human prion detection in cerebrospinal fluid by real-time quaking-induced conversion. Nat Med 17(2):175–178. PubMed PMID: 21278748

    Article  CAS  PubMed  Google Scholar 

  • Ballard C, Gauthier S, Corbett A, Brayne C, Aarsland D, Jones E (2011) Alzheimer’s disease. Lancet 377(9770):1019–1031. PubMed PMID: 21371747

    Article  PubMed  Google Scholar 

  • Blennow K, Fredman P, Wallin A, Gottfries CG, Karlsson I, Langstrom G et al (1993) Protein analysis in cerebrospinal fluid. II. Reference values derived from healthy individuals 18–88 years of age. Eur Neurol 33(2):129–133. PubMed PMID: 8467819

    Article  CAS  PubMed  Google Scholar 

  • Boer K, Isenmann S, Deufel T (2007) Strong interference of hemoglobin concentration on CSF total protein measurement using the trichloroacetic acid precipitation method. Clin Chem Lab Med 45(1):112–113. PubMed PMID: 17243927

    Article  CAS  PubMed  Google Scholar 

  • Breebaart K, Becker H, Jongebloed FA (1978) Investigation of reference values of components of cerebrospinal fluid. J Clin Chem Clin Biochem 16(10):561–565. PubMed PMID: 712336

    CAS  PubMed  Google Scholar 

  • Brettschneider J, Claus A, Kassubek J, Tumani H (2005) Isolated blood-cerebrospinal fluid barrier dysfunction: prevalence and associated diseases. J Neurol 252(9):1067–1073. PubMed PMID: 15789126

    Article  CAS  PubMed  Google Scholar 

  • Buruma OJ, Janson HL, Den Bergh FA, Bots GT (1981) Blood-stained cerebrospinal fluid: traumatic puncture or haemorrhage? J Neurol Neurosurg Psychiatry 44(2):144–147. PubMed PMID: 7217970. Pubmed Central PMCID: 490846

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Collins SJ, Sanchez-Juan P, Masters CL, Klug GM, van Duijn C, Poleggi A et al (2006) Determinants of diagnostic investigation sensitivities across the clinical spectrum of sporadic Creutzfeldt-Jakob disease. Brain 129(Pt 9):2278–2287. PubMed PMID: 16816392

    Article  CAS  PubMed  Google Scholar 

  • Coulthart MB, Jansen GH, Olsen E, Godal DL, Connolly T, Choi BC et al (2011) Diagnostic accuracy of cerebrospinal fluid protein markers for sporadic Creutzfeldt-Jakob disease in Canada: a 6-year prospective study. BMC Neurol 11:133. PubMed PMID: 22032272. Pubmed Central PMCID: 3216246

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Cruickshank A, Beetham R, Holbrook I, Watson I, Wenham P, Keir G et al (2005) Spectrophotometry of cerebrospinal fluid in suspected subarachnoid haemorrhage. BMJ 330(7483):138. PubMed PMID: 15649929. Pubmed Central PMCID: 544435

    Article  PubMed Central  PubMed  Google Scholar 

  • Cruickshank A, Auld P, Beetham R, Burrows G, Egner W, Holbrook I et al (2008) Revised national guidelines for analysis of cerebrospinal fluid for bilirubin in suspected subarachnoid haemorrhage. Ann Clin Biochem 45(Pt 3):238–244. PubMed PMID: 18482910

    Article  CAS  PubMed  Google Scholar 

  • Danise P, Maconi M, Rovetti A, Avino D, Di Palma A, Gerardo Pirofalo M et al (2013) Cell counting of body fluids: comparison between three automated haematology analysers and the manual microscope method. Int J Lab Hematol 35(6):608–613. PubMed PMID: 23647736

    Article  CAS  PubMed  Google Scholar 

  • Davies G, Keir G, Thompson EJ, Giovannoni G (2003) The clinical significance of an intrathecal monoclonal immunoglobulin band: a follow-up study. Neurology 60(7):1163–1166. PubMed PMID: 12682325

    Article  PubMed  Google Scholar 

  • Deisenhammer F, Bartos A, Egg R, Gilhus NE, Giovannoni G, Rauer S et al (2006) Guidelines on routine cerebrospinal fluid analysis. Report from an EFNS task force. Eur J Neurol 13(9):913–922. PubMed PMID: 16930354

    Article  CAS  PubMed  Google Scholar 

  • Deisenhammer F, Egg R, Giovannoni G, Hemmer B, Petzold A, Sellebjerg F et al (2009) EFNS guidelines on disease-specific CSF investigations. Eur J Neurol 16(6):760–770. PubMed PMID: 19475759

    Article  CAS  PubMed  Google Scholar 

  • del Campo M, Mollenhauer B, Bertolotto A, Engelborghs S, Hampel H, Simonsen AH et al (2012) Recommendations to standardize preanalytical confounding factors in Alzheimer’s and Parkinson’s disease cerebrospinal fluid biomarkers: an update. Biomark Med 6(4):419–430. PubMed PMID: 22917144

    Article  PubMed  Google Scholar 

  • Delank HW (1972) Clinical cerebrospinal fluid diagnostics. Nervenarzt 43(2):57–68. PubMed PMID: 4622639. Klinische Liquordiagnostik

    CAS  PubMed  Google Scholar 

  • Desplat-Jego S, Feuillet L, Pelletier J, Bernard D, Cherif AA, Boucraut J (2005) Quantification of immunoglobulin free light chains in cerebrospinal fluid by nephelometry. J Clin Immunol 25(4):338–345. PubMed PMID: 16133990

    Article  CAS  PubMed  Google Scholar 

  • DGLN DGfLuKN (2004) Ausgewählte Methoden der Liquordiagnostik und Klinischen Neurochemie. http://www.uke.de/extern/dgln/pdf/Methodenkatalog.pdf

  • Diener H, Weimar C, Berlit P, Deuschl G, Elger C, Gold R, et al. Leitlinen für Diagnostik und Therapie in der Neurologie. 2012;5. Available from: http://www.dgn.org/images/stories/dgn/leitlinien/LL_2012/pdf/ll_34_ambulant_erworbene_bakterielle_eitrige_meningoenzephalitis.pdf

  • Dobson R, Ramagopalan S, Davis A, Giovannoni G (2013) Cerebrospinal fluid oligoclonal bands in multiple sclerosis and clinically isolated syndromes: a meta-analysis of prevalence, prognosis and effect of latitude. J Neurol Neurosurg Psychiatry 84(8):909–914. PubMed PMID: 23431079

    Article  PubMed  Google Scholar 

  • Dubois B, Feldman HH, Jacova C, Dekosky ST, Barberger-Gateau P, Cummings J et al (2007) Research criteria for the diagnosis of Alzheimer’s disease: revising the NINCDS-ADRDA criteria. Lancet Neurol 6(8):734–746. PubMed PMID: 17616482

    Article  PubMed  Google Scholar 

  • Dufour-Rainfray D, Beaufils E, Vourc’h P, Vierron E, Mereghetti L, Gendrot C et al (2013) Total protein level in cerebrospinal fluid is stable in elderly adults. J Am Geriatr Soc 61(10):1819–1821. PubMed PMID: 24117297

    PubMed  Google Scholar 

  • Dujmovic I, Deisenhammer F (2010) Stability of cerebrospinal fluid/serum glucose ratio and cerebrospinal fluid lactate concentrations over 24 h: analysis of repeated measurements. Clin Chem Lab Med 48(2):209–212. PubMed PMID: 19958211

    Article  CAS  PubMed  Google Scholar 

  • Duranti F, Pieri M, Centonze D, Buttari F, Bernardini S, Dessi M (2013) Determination of kappaFLC and kappa Index in cerebrospinal fluid: a valid alternative to assess intrathecal immunoglobulin synthesis. J Neuroimmunol 263(1–2):116–120. PubMed PMID: 23916392

    Article  CAS  PubMed  Google Scholar 

  • Eeg-Olofsson O, Link H, Wigertz A (1981) Concentrations of CSF proteins as a measure of blood brain barrier function and synthesis of IgG within the CNS in ‘normal’ subjects from the age of 6 months to 30 years. Acta Paediatr Scand 70(2):167–170. PubMed PMID: 7234400

    Article  CAS  PubMed  Google Scholar 

  • Freedman MS, Thompson EJ, Deisenhammer F, Giovannoni G, Grimsley G, Keir G et al (2005) Recommended standard of cerebrospinal fluid analysis in the diagnosis of multiple sclerosis: a consensus statement. Arch Neurol 62(6):865–870. PubMed PMID: 15956157

    Article  PubMed  Google Scholar 

  • Garton MJ, Keir G, Lakshmi MV, Thompson EJ (1991) Age-related changes in cerebrospinal fluid protein concentrations. J Neurol Sci 104(1):74–80. PubMed PMID: 1717663

    Article  CAS  PubMed  Google Scholar 

  • Gilland O (1967) Lumbar cerebrospinal fluid total protein in healthy subjects. Acta Neurol Scand 43(4):526–529. PubMed PMID: 6081215

    Article  CAS  PubMed  Google Scholar 

  • Hegen H, Auer M, Deisenhammer F (2014) Serum glucose adjusted cut-off values for normal cerebrospinal fluid/serum glucose ratio: implications for clinical practice. Clin Chem Lab Med 52(9):1335–1340. PubMed PMID: 24731954

    Article  CAS  PubMed  Google Scholar 

  • Hort J et al (2010) EFNS guidelines for the diagnosis and management of Alzheimer’s disease. Eur J Neurol 17:1236–1248

    Article  CAS  PubMed  Google Scholar 

  • Khademi M, Kockum I, Andersson ML, Iacobaeus E, Brundin L, Sellebjerg F et al (2011) Cerebrospinal fluid CXCL13 in multiple sclerosis: a suggestive prognostic marker for the disease course. Mult Scler 17(3):335–343. PubMed PMID: 21135023

    Article  CAS  PubMed  Google Scholar 

  • Krakauer M, Schaldemose Nielsen H, Jensen J, Sellebjerg F (1998) Intrathecal synthesis of free immunoglobulin light chains in multiple sclerosis. Acta Neurol Scand 98(3):161–165. PubMed PMID: 9786611

    Article  CAS  PubMed  Google Scholar 

  • Ladogana A, Puopolo M, Croes EA, Budka H, Jarius C, Collins S et al (2005) Mortality from Creutzfeldt-Jakob disease and related disorders in Europe, Australia, and Canada. Neurology 64(9):1586–1591. PubMed PMID: 15883321

    Article  CAS  PubMed  Google Scholar 

  • Lang DT, Berberian LB, Lee S, Ault M (1990) Rapid differentiation of subarachnoid hemorrhage from traumatic lumbar puncture using the D-dimer assay. Am J Clin Pathol 93(3):403–405. PubMed PMID: 2309662

    CAS  PubMed  Google Scholar 

  • Leen WG, Willemsen MA, Wevers RA, Verbeek MM (2012) Cerebrospinal fluid glucose and lactate: age-specific reference values and implications for clinical practice. PLoS One 7(8):e42745. PubMed PMID: 22880096. Pubmed Central PMCID: 3412827

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Leschziner G (2014) Narcolepsy: a clinical review. Pract Neurol 15. PubMed PMID: 24830461

    Google Scholar 

  • Marton KI, Gean AD (1986) The spinal tap: a new look at an old test. Ann Intern Med 104(6):840–848. PMID: 3518565

    Article  CAS  PubMed  Google Scholar 

  • McKhann GM, Knopman DS, Chertkow H, Hyman BT, Jack CR Jr, Kawas CH et al (2011) The diagnosis of dementia due to Alzheimer’s disease: recommendations from the National Institute on Aging-Alzheimer’s Association workgroups on diagnostic guidelines for Alzheimer’s disease. Alzheimers Dement 7(3):263–269. PubMed PMID: 21514250. Pubmed Central PMCID: 3312024

    Article  PubMed Central  PubMed  Google Scholar 

  • Meissner B, Westner IM, Kallenberg K, Krasnianski A, Bartl M, Varges D et al (2005) Sporadic Creutzfeldt-Jakob disease: clinical and diagnostic characteristics of the rare VV1 type. Neurology 65(10):1544–1550. PubMed PMID: 16221949

    Article  CAS  PubMed  Google Scholar 

  • Mertin J, Wisser H, Doerr P (1971) [Studies on the normal concentration range of total protein and the total protein fractions of the cerebrospinal fluid by electrophoresis on cellulose acetate (author’s transl)]. Z Klin Chem Klin Biochem 9(4):337–340. PubMed PMID: 4142825. Untersuchung uber den Normalbereich des Gesamteiweisses und der Eiweissfraktionen des Liquor cerebrospinalis nach elektrophoretischer Trennung auf Celluloseacetatfolie

    CAS  PubMed  Google Scholar 

  • Miller DH, Weinshenker BG, Filippi M, Banwell BL, Cohen JA, Freedman MS et al (2008) Differential diagnosis of suspected multiple sclerosis: a consensus approach. Mult Scler 14(9):1157–1174. PubMed PMID: 18805839. Pubmed Central PMCID: 2850590

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Morris JC, Blennow K, Froelich L, Nordberg A, Soininen H, Waldemar G et al (2014) Harmonized diagnostic criteria for Alzheimer’s disease: recommendations. J Intern Med 275(3):204–213. PubMed PMID: 24605805

    Article  CAS  PubMed  Google Scholar 

  • Muayqil T, Gronseth G, Camicioli R (2012) Evidence-based guideline: diagnostic accuracy of CSF 14-3-3 protein in sporadic Creutzfeldt-Jakob disease: report of the guideline development subcommittee of the American Academy of Neurology. Neurology 79(14):1499–1506. PubMed PMID: 22993290. Pubmed Central PMCID: 3525296

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Mygland A, Ljostad U, Fingerle V, Rupprecht T, Schmutzhard E, Steiner I et al (2010) EFNS guidelines on the diagnosis and management of European Lyme neuroborreliosis. Eur J Neurol 17(1):8–16, e1–4. PubMed PMID: 19930447

    Article  CAS  PubMed  Google Scholar 

  • Nagy K, Skagervik I, Tumani H, Petzold A, Wick M, Kuhn HJ et al (2013) Cerebrospinal fluid analyses for the diagnosis of subarachnoid haemorrhage and experience from a Swedish study. What method is preferable when diagnosing a subarachnoid haemorrhage? Clin Chem Lab Med 51(11):2073–2086. PubMed PMID: 23729569

    Article  CAS  PubMed  Google Scholar 

  • Nakano T, Matsui M, Inoue I, Awata T, Katayama S, Murakoshi T (2011) Free immunoglobulin light chain: its biology and implications in diseases. Clin Chim Aacta 412(11–12):843–849. PubMed PMID: 21396928

    Article  CAS  Google Scholar 

  • Olischer RM, von Suchodoletz W (1972) [Evaluation of quantitative cell findings in cerebrospinal fluid containing blood]. Z Arztl Fortbild 66(4):213–218. PubMed PMID: 5036811. Zur Bewertung quantitativer Zellbefunde im bluthaltigen Liquor cerebrospinalis

    CAS  Google Scholar 

  • Otto M, Bowser R, Turner M, Berry J, Brettschneider J, Connor J et al (2012) Roadmap and standard operating procedures for biobanking and discovery of neurochemical markers in ALS. Amyotroph Lateral Scler 13(1):1–10. PubMed PMID: 22214350

    Article  PubMed  Google Scholar 

  • Page KB, Howell SJ, Smith CM, Dabbs DJ, Malia RG, Porter NR et al (1994) Bilirubin, ferritin, D-dimers and erythrophages in the cerebrospinal fluid of patients with suspected subarachnoid haemorrhage but negative computed tomography scans. J Clin Pathol 47(11):986–989. PubMed PMID: 7829694. Pubmed Central PMCID: 503057

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Petzold A, Keir G, Sharpe TL (2005) Why human color vision cannot reliably detect cerebrospinal fluid xanthochromia. Stroke 36(6):1295–1297. PubMed PMID: 15879320

    Article  PubMed  Google Scholar 

  • Pfausler B, Beer R, Engelhardt K, Kemmler G, Mohsenipour I, Schmutzhard E (2004) Cell index–a new parameter for the early diagnosis of ventriculostomy (external ventricular drainage)-related ventriculitis in patients with intraventricular hemorrhage? Acta Neurochir 146(5):477–481. PubMed PMID: 15118885

    Article  CAS  PubMed  Google Scholar 

  • Plebani M (2006) Errors in clinical laboratories or errors in laboratory medicine? Clin Chem Lab Med 44(6):750–759. PubMed PMID: 16729864

    CAS  PubMed  Google Scholar 

  • Polman CH, Reingold SC, Banwell B, Clanet M, Cohen JA, Filippi M et al (2011) Diagnostic criteria for multiple sclerosis: 2010 revisions to the McDonald criteria. Ann Neurol 69(2):292–302. PubMed PMID: 21387374. Pubmed Central PMCID: 3084507

    Article  PubMed Central  PubMed  Google Scholar 

  • Reiber H (1994) Flow rate of cerebrospinal fluid (CSF)–a concept common to normal blood-CSF barrier function and to dysfunction in neurological diseases. J Neurol Sci 122(2):189–203. PubMed PMID: 8021703

    Article  CAS  PubMed  Google Scholar 

  • Reiber H, Lange P (1991) Quantification of virus-specific antibodies in cerebrospinal fluid and serum: sensitive and specific detection of antibody synthesis in brain. Clin Chem 37(7):1153–1160. PubMed PMID: 1855284

    CAS  PubMed  Google Scholar 

  • Reiber H, Peter JB (2001) Cerebrospinal fluid analysis: disease-related data patterns and evaluation programs. J Neurol Sci 184(2):101–122. PubMed PMID: 11239944

    Article  CAS  PubMed  Google Scholar 

  • Reiber H, Ungefehr S, Jacobi C (1998) The intrathecal, polyspecific and oligoclonal immune response in multiple sclerosis. Mult Scler 4(3):111–117. PubMed PMID: 9762657

    Article  CAS  PubMed  Google Scholar 

  • Reiber H, Otto M, Trendelenburg C, Wormek A (2001) Reporting cerebrospinal fluid data: knowledge base and interpretation software. Clin Chem Lab Med 39(4):324–332. PubMed PMID: 11388657

    Article  CAS  PubMed  Google Scholar 

  • Reske A, Haferkamp G, Hopf HC (1981) Influence of artificial blood contamination of the analysis of cerebrospinal fluid. J Neurol 226(3):187–193. PubMed PMID: 6172566

    Article  CAS  PubMed  Google Scholar 

  • Rudick RA, French CA, Breton D, Williams GW (1989) Relative diagnostic value of cerebrospinal fluid kappa chains in MS: comparison with other immunoglobulin tests. Neurology 39(7):964–968. PubMed PMID: 2500621

    Article  CAS  PubMed  Google Scholar 

  • Sandhaus LM, Ciarlini P, Kidric D, Dillman C, O’Riordan M (2010) Automated cerebrospinal fluid cell counts using the Sysmex XE-5000: is it time for new reference ranges? Am J Clin Pathol 134(5):734–738. PubMed PMID: 20959656

    Article  PubMed  Google Scholar 

  • Senel M, Tumani H, Lauda F, Presslauer S, Mojib-Yezdani R, Otto M et al (2014) Cerebrospinal fluid immunoglobulin kappa light chain in clinically isolated syndrome and multiple sclerosis. PLoS One 9(4):e88680. PubMed PMID: 24695382. Pubmed Central PMCID: 3973621

    Article  PubMed Central  PubMed  Google Scholar 

  • Shah KH, Edlow JA (2002) Distinguishing traumatic lumbar puncture from true subarachnoid hemorrhage. J Emerg Med 23(1):67–74. PubMed PMID: 12217474

    Article  PubMed  Google Scholar 

  • Sharief MK, Keir G, Thompson EJ (1990) Intrathecal synthesis of IgM in neurological diseases: a comparison between detection of oligoclonal bands and quantitative estimation. J Neurol Sci 96(2–3):131–142. PubMed PMID: 2115907

    Article  CAS  PubMed  Google Scholar 

  • Sorbi S, Hort J, Erkinjuntti T, Fladby T, Gainotti G, Gurvit H et al (2012) EFNS-ENS Guidelines on the diagnosis and management of disorders associated with dementia. Eur J Neurol 19(9):1159–1179. PubMed PMID: 22891773

    Article  CAS  PubMed  Google Scholar 

  • Spanos A, Harrell FE Jr, Durack DT (1989) Differential diagnosis of acute meningitis. An analysis of the predictive value of initial observations. JAMA 262(19):2700–2707. PubMed PMID: 2810603

    Article  CAS  PubMed  Google Scholar 

  • Sperling RA, Aisen PS, Beckett LA, Bennett DA, Craft S, Fagan AM et al (2011) Toward defining the preclinical stages of Alzheimer’s disease: recommendations from the National Institute on Aging-Alzheimer’s Association workgroups on diagnostic guidelines for Alzheimer’s disease. Alzheimers Dement 7(3):280–292. PubMed PMID: 21514248. Pubmed Central PMCID: 3220946

    Article  PubMed Central  PubMed  Google Scholar 

  • Steele RW, Marmer DJ, O’Brien MD, Tyson ST, Steele CR (1986) Leukocyte survival in cerebrospinal fluid. J Clin Microbiol 23(5):965–966. PubMed PMID: 3711287. Pubmed Central PMCID: 268763

    CAS  PubMed Central  PubMed  Google Scholar 

  • Teunissen CE, Petzold A, Bennett JL, Berven FS, Brundin L, Comabella M et al (2009) A consensus protocol for the standardization of cerebrospinal fluid collection and biobanking. Neurology 73(22):1914–1922. PubMed PMID: 19949037. Pubmed Central PMCID: 2839806

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Teunissen C, Menge T, Altintas A, Alvarez-Cermeno JC, Bertolotto A, Berven FS et al (2013) Consensus definitions and application guidelines for control groups in cerebrospinal fluid biomarker studies in multiple sclerosis. Mult Scler 19(13):1802–1809. PubMed PMID: 23695446

    Article  PubMed  Google Scholar 

  • Tibbling G, Link H, Ohman S (1977) Principles of albumin and IgG analyses in neurological disorders. I. Establishment of reference values. Scand J Clin Lab Invest 37(5):385–390. PubMed PMID: 337459

    Article  CAS  PubMed  Google Scholar 

  • Tumani H, Deisenhammer F, Giovannoni G, Gold R, Hartung HP, Hemmer B et al (2011) Revised McDonald criteria: the persisting importance of cerebrospinal fluid analysis. Ann Neurol 70(3):520; author reply 1. PubMed PMID: 21710627

    Article  PubMed  Google Scholar 

  • Vanderstichele H, Bibl M, Engelborghs S, Le Bastard N, Lewczuk P, Molinuevo JL et al (2012) Standardization of preanalytical aspects of cerebrospinal fluid biomarker testing for Alzheimer’s disease diagnosis: a consensus paper from the Alzheimer’s Biomarkers Standardization Initiative. Alzheimers Dement 8(1):65–73. PubMed PMID: 22047631

    Article  CAS  PubMed  Google Scholar 

  • Walton J (1956) Subarachnoid hemorrhage. E & S Livingstone Ltd., Edinburgh

    Google Scholar 

  • Watson ID, Beetham R, Fahie-Wilson MN, Holbrook IB, O’Connell DM (2008) What is the role of cerebrospinal fluid ferritin in the diagnosis of subarachnoid haemorrhage in computed tomography-negative patients? Ann Clin Biochem 45(Pt 2):189–192. PubMed PMID: 18325184

    Article  CAS  PubMed  Google Scholar 

  • Weisner B, Bernhardt W (1978) Protein fractions of lumbar, cisternal, and ventricular cerebrospinal fluid. Separate areas of reference. J Neurol Sci 37(3):205–214. PubMed PMID: 681976

    Article  CAS  PubMed  Google Scholar 

  • Zimmermann M, Ruprecht K, Kainzinger F, Heppner FL, Weimann A (2011) Automated vs. manual cerebrospinal fluid cell counts: a work and cost analysis comparing the Sysmex XE-5000 and the Fuchs-Rosenthal manual counting chamber. Int J Lab Hematol 33(6):629–637. PubMed PMID: 21668655

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Neurology, Innsbruck Medical University, Anichstrasse 35, Innsbruck, 6020, Austria

    Harald Hegen

  2. Neurochemistry Laboratory and Biobank, Department of Clinical Chemistry, VU University Medical Center Amsterdam, Amsterdam, The Netherlands

    Charlotte E. Teunissen

  3. Danish Multiple Sclerosis Center, Department of Neurology Rigshospitalet, University of Copenhagen, Copenhagen, Denmark

    Finn Sellebjerg

  4. Department of Neurology, University of Ulm, Ulm, Germany

    Hayrettin Tumani

  5. Department of Neurology, Innsbruck Medical University, Innsbruck, Austria

    Florian Deisenhammer

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  1. Harald Hegen
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  2. Charlotte E. Teunissen
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  3. Finn Sellebjerg
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  4. Hayrettin Tumani
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  5. Florian Deisenhammer
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Correspondence to Harald Hegen .

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Editors and Affiliations

  1. Department of Neurology, Innsbruck Medical University, Innsbruck, Austria

    Florian Deisenhammer

  2. Department of Neurology, Danish Multiple Sclerosis Center Rigshospitalet, University of Copenhagen, Copenhagen, Denmark

    Finn Sellebjerg

  3. Department of Clinical Chemistry, VU University Medical Center Amsterdam Neurochemistry Laboratory and Biobank, Amsterdam, The Netherlands

    Charlotte E Teunissen

  4. Department of Neurology, University of Ulm, Ulm, Germany

    Hayrettin Tumani

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Hegen, H., Teunissen, C.E., Sellebjerg, F., Tumani, H., Deisenhammer, F. (2015). Guidelines on Cerebrospinal Fluid Analysis. In: Deisenhammer, F., Sellebjerg, F., Teunissen, C., Tumani, H. (eds) Cerebrospinal Fluid in Clinical Neurology. Springer, Cham. https://doi.org/10.1007/978-3-319-01225-4_24

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