Abstract
Majority of the human cultures depend on many plants as a valuable source of medicines in various worldwide traditional medicinal systems. The utilization of these plants is increasing rapidly worldwide with the growing demand for natural products and drugs. Therefore, many plants are now threatened with extinction in the past decades due to severe human interference. Pharmacologically significant plants should be conserved from overexploitation, uncontrolled collection, heavy deforestation, and habitat destruction. For the large-scale propagation of such medicinal plants, conventional methods become difficult as pharmaceutical industries require. There is a great surge of public interest in in vitro propagation of endangered plants for conservation strategies and the mass production of secondary metabolites through different culture methods. Thus, the in vitro plant tissue culture techniques are efficient plant regeneration methods for the sustainable production of pharmacologically important plant species. So, the conservation of endangered medicinal plants having medicinal properties is necessary for future generations. This chapter reviews the in vitro propagation and conservation of some important endangered medicinal plants with special emphasis on their pharmacological activities. These micropropagation techniques are vital for producing disease-free plant materials with desirable pharmacological features.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Roberson E (2008) Nature’s pharmacy, our treasure chest: why we must conserve our natural heritage. Centre for Biological Diversity. www.Biologicaldiversity.Org
Piet JH (1952) Logical presentation of the Saiva Siddhanta philosophy. Christian Literature Society for India, Madras
Nalawade SM, Sagare AP, Lee CY, Kao CL, Tsay HS (2003) Studies on tissue culture of Chinese medicinal plant resources in Taiwan and their sustainable utilization. Bot Bull Acad Sin 44(2):79–98
Pimm S, Russell G, Gittleman J, Brooks T (1995) The future of biodiversity. Science 269:347
Raven PH (1987) The scope of plant conservation problem worldwide. In: Bramwell D, Hamann O, Heywood V, Synge H (eds) Botanical gardens and the world conservation strategy. Academic, London, pp 19–20
Schemske DW, Husband BC, Ruckelshaus MH, Goodwillie C, Parker IM, Bishop JG (1994) Evaluating approaches to the conservation of rare and endangered plants. Ecology 75:584–606
Cole IB, Saxena PK, Murch SJ (2007) Medicinal biotechnology in the genus Scutellaria. In Vitro Cell Dev Biol-Plant 43(4):318–327
Mendelsohn R, Balick MJ (1995) The value of undiscovered pharmaceuticals in tropical forests. Econ Bot 49(2):223–228
Romanelli C, Cooper D, Campbell-Lendrum D, Maiero M, Karesh WB, Hunter D, Golden CD (2015) Connecting global priorities: biodiversity and human health: a state of knowledge review. World Health Organistion/Secretariat of the UN Convention on Biological Diversity
Gopi DK, Mattummal R, Narayana SKK, Parameswaran S (2018) IUCN red listed medicinal plants of siddha. J Res Siddha Med 1(1):15
Benzie IF, Wachtel-Galor S (eds) (2011) Herbal medicine: biomolecular and clinical aspects, 2nd edn. CRC Press/Taylor & Francis, Boca Raton
Singh RP, Gupta MK (1990) Soil and vegetation study of Lahaul and Spiti cold desert of Western Himalayas. Indian Forester 116(10):785–790
Fox JL, Nurbu C, Bhatt S, Chandola A (1994) Wildlife conservation and land-use changes in the Transhimalayan region of Ladakh, India. Mt Res Dev 14:39–60
Dhyani A (2015) Critically endangered Indian medicinal plants. Heritage Amruth 11:42–45
Singh PR, Singh LJ (2021) In-vitro propagation for improvement of medicinal plants: a review. J Pharmacogn Phytochem 10(1):1484–1489
Kala CP, Dhyani PP, Sajwan BS (2006) Developing the medicinal plants sector in northern India: challenges and opportunities. J Ethnobiol Ethnomed 2(1):1–15
Ramakrishnan R, Periyasamy R, Lakkakula S, Subramani P, Arockiam SR, Manikandan R (2017) In vitro propagation and conservation of useful endangered medicinal plants with anticancer activity. J Mol Biol Biotechnol 2(3):8
Bourgaud F, Gravot A, Milesi S, Gontier E (2001) Production of plant secondary metabolites: a historical perspective. Plant Sci 161(5):839–851
Oseni OM, Pande V, Nailwal TK (2018) A review on plant tissue culture, a technique for propagation and conservation of endangered plant species. Int J Curr Microbiol App Sci 7(7):3778–3786
Chokheli VA, Dmitriev PA, Rajput VD, Bakulin SD, Azarov AS, Varduni TV, Stepanenko VV, Tarigholizadeh S, Singh RK, Verma KK, Minkina TM (2020) Recent development in micropropagation techniques for rare plant species. Plants 9(12):1733
Sharma S, Thokchom R (2014) A review on endangered medicinal plants of India and their conservation. J Crop Weed 10(2):205–218
Gutiérrez RM, Orchids P (2010) A review of uses in traditional medicine, its phytochemistry and pharmacology. J Med Plants Res 4(8):592–638
Gailīte A, Kļaviņa D, Ievinsh G (2010) In vitro propagation of an endangered plant Saussurea esthonica. Environ Expe Biol 8:43–48
Kone M, Koné T, Silué N, Soumahoro AB, Kouakou TH (2015) In vitro seeds germination and seedling growth of Bambara groundnut (Vigna subterranea (L.) Verdc (Fabaceae)). Sci World J 2015:595073
Utami ESW, Hariyanto S, Manuhara YSW (2017) In vitro propagation of the endangered medicinal orchid, Dendrobium lasianthera through mature seed culture. Asian Pac J Trop Biomed 7(5):406–410
An J, Kim PB, Park HB, Kim S, Park HJ, Lee CW et al (2021) Effects of different growth media on in vitro seedling development of an endangered orchid species Sedirea japonica. Plan Theory 10(6):1193
Hesami M, Daneshvar MH (2018) Indirect organogenesis through seedling-derived leaf segments of Ficus religiosa- a multipurpose woody medicinal plant. J Crop Sci Biotechnol 21(2):129–136
Shatnawi M, Abubaker S, Odat N, Al-Tawaha AR, Majdalawi M (2021) Antimicrobial activity and micropropagation of some rare and endangered plants of Jordan. J Ecol Eng 22(6):151
Yadav KN, Kadam PV, Patel JA, Patil MJ (2014) Strychnos potatorum: phytochemical and pharmacological review. Pharmacogn Rev 8(15):61
Kagithoju S, Godishala V, Kairamkonda M, Nanna RS (2013) Embryo culture is an efficient way to conserve a medicinally important endangered forest tree species Strychnos potatorum. J For Res 24(2):279–283
Rambabu M, Upender M, Ujjwala D, Ugandhar T, Praveen M, Rama Swamy N (2006) In vitro zygotic embryo culture of an endangered forest tree Givotia rottleriformis and factors affecting its germination and seedling growth. In Vitro Cell Dev Biol-Plant 42(5):418–421
Askari-Khorasgani O, Mortazaeinezhad F, Otroshy M, Golparvar AR, Moeini A (2013) Direct regeneration of an endangered medicinal plant Kelussia odoratissima. Int J Agric Crop Sci 5(17):1969
Talari S, Penchala S, Marka R, Rudroju S, Ramaswamy N (2013) Embryo culture an efficient tool for conservation of an endangered medicinally important forest tree Oroxylum indicum Kurz. Int J Biotechnol Res 3:45–52
Kushalan S, Yathisha UG, Khyahrii SA, Hegde S (2022) Phytochemical and antioxidant evaluation of in vitro and in vivo propagated plants of Curculigo orchioides. In Vitro Cell Dev Biol-Plant 11:1–10
Chauhan NS, Sharma V, Thakur M, Dixit VK (2010) Curculigo orchioides: the black gold with numerous health benefits. J Chin Integr Med 8(7):613–623
Almemary A (2020) Callus induction and differentiation. Future Agric 3:5–9
Benjamin ED, Ishaku GA, Peingurta FA, Afolabi AS (2019) Callus culture for the production of therapeutic compounds. Am J Plant Biol 4(4):76–84
Mitra GC, Chaturvedi HC (1970) Fruiting plants from in vitro grown leaf tissue of Rauvolfia serpentina Benth. Curr Sci 39:128–129
Bakhtiar Z, Mirjalili MH, Sonboli A (2016) In vitro callus induction and micropropagation of Thymus persicus (Lamiaceae), an endangered medicinal plant. Crop Breed Appl Biotechnol 16(1):48–54
Purohit S, Joshi K, Rawat V, Bhatt ID, Nandi SK (2020) Efficient plant regeneration through callus in Zanthoxylum armatum DC: an endangered medicinal plant of the Indian Himalayan region. Plant Biosyst Int J Deal All Aspects Plant Biol 154(3):288–294
Kumar N, Reddy MP (2011) In vitro plant propagation: a review. J Forest Environ Sci 27(2):61–72
Rout GR, Samantaray S, Das P (2000) In vitro manipulation and propagation of medicinal plants. Biotechnol Adv 18(2):91–120
Wala BB, Jasrai YT (2003) Micropropagation of an endangered medicinal plant: Curculigo orchioides Gaertn. Plant Tissue Culture 13(1):13–19
Ismail MF, Abdulla NP, Saleh GB, Ismail M (2010) Anthesis and flower visitors in Curculigo latifolia Dryand. J Biol Life Sci 1:13–15
Kant R (2005) Sweet proteins–potential replacement for artificial low-calorie sweeteners. Nutr J 4(1):1–6
Ali MF, Ratnam W, Heng LY (2007) Genetic effects of arsenic and heavy metals pollutants on Curculigo latifolia Lumbah. J Biol Sci 7:1155–1162
Babaei N, Psyquay Abdullah NA, Saleh G, Lee Abdullah T (2014) An efficient in vitro plantlet regeneration from shoot tip cultures of Curculigo latifolia, a medicinal plant. Sci World J 2014:275028
Kaushal S, Sidana A, Dev K (2014) In-vitro plant production through apical meristem culture of Gentiana kurroo Royle. J Med Plants Stud 3(1):4–9
Pe PP, Naing AH, Soe MT, Kang H, Park KI, Kim CK (2020) Establishment of meristem culture for virus-free and genetically stable production of the endangered plant Hosta capitata. Sci Hortic 272:109591
Bjowani SS, Rasdan MK (1983) Plant tissue culture: theory and practice. Developments in crop science, Elsevier Science Publishers, Amsterdam, The Netherlands. 1–373
Thomas T, Isolation D (2009) Callus formation and plantlet regeneration from mesophyll protoplasts of Tylophora indica (Burm. F.) Merrill: an important medicinal plant. In Vitro Cell Dev Biol-Plant 45(5):591–598
Cai Y, Liu Y, Liu Z, Zhang F, Xiang F, Xia G (2009) High-frequency embryogenesis and regeneration of plants with high content of gentiopicroside from the Chinese medicinal plant Gentiana straminea maxim. In-Vitro Cell Dev Biol-Plant 45(6):730–739
Shi G, Yang L, He T (2016) Plant regeneration from protoplasts of Gentiana straminea maxim. Open Life Sci 11(1):55–60
Kaur R, Kashyap A, Majeed S, Chauhan NS (2010) In vitro propagation and conservation of Inula racemosa Hook. F. an endangered medicinal plant of temperate origin. J Adv Lab Res Biol 1(1):67–70
Dohling S, Kumaria S, Tandon P (2012) Multiple shoot induction from axillary bud cultures of the medicinal orchid, Dendrobium longicornu. AoB Plants 2012:pls032
Rameshkumar R, Largia MJ, Satish L, Shilpha J, Ramesh M (2017) In-vitro mass propagation and conservation of Nilgirianthus ciliatus through nodal explants: a globally endangered, high trade medicinal plant of Western Ghats. Plant Biosyst Int J Deal All Aspects Plant Biol 151(2):204–211
Khan A, Shah AH, Ali N (2021) In-vitro propagation and phytochemical profiling of a highly medicinal and endemic plant species of the Himalayan region (Saussurea costus). Sci Rep 11(1):1–13
Nam NB, Trieu LN, Vu NT, Trung LH, Tra TTH, Tram LT, Dai PH, Tung HT, Nhut DT (2022) Micropropagation of Jasminanthes tuyetanhiae: an endemic and valuable herb in Vietnam. Plant Cell Tissue Organ Cult 148(1):35–44
Komakech R, Kim YG, Kim WJ, Omujal F, Yang S, Moon BC, Okello D, Rahmat E, Nambatya Kyeyune G, Matsabisa MG, Kang Y (2020) A micropropagation protocol for the endangered medicinal tree Prunus africana (Hook f.) Kalkman: genetic fidelity and physiological parameter assessment. Front Plant Sci 11:1871
Camper ND, Coker PS, Wedge DE, Keese RJ (1997) In-vitro culture of ginkgo. In-Vitro Cell Dev Biol-Plant 33(2):125–127
Jayanthi M, Mandal PK (2001) Plant regeneration through somatic embryogenesis and RAPD analysis of regenerated plants in Tylophora indica (Burm. f. Merrill.). In-Vitro Cell Dev Biol-Plant 37(5):576–580
Chand S, Sahrawat AK (2002) Somatic embryogenesis and plant regeneration from root segments of Psoralea corylifolia L., an endangered medicinally important plant. In-Vitro Cell Dev Biol-Plant 38(1):33–38
Tawfik AA, Noga G (2002) Cumin regeneration from seedling derived embryogenic callus in response to amended kinetin. Plant Cell Tissue Organ Cult 69(1):35–40
Joshee N, Biswas BK, Yadav AK (2007) Somatic embryogenesis and plant development in Centella asiatica L., a highly prized medicinal plant of the tropics. Hortic Sci 42(3):633–637
Garro-Monge G, Gatica-Arias AM, Valdez-Melara M (2008) Somatic embryogenesis, plant regeneration and acemannan detection in aloe (Aloe barbadensis Mill.). Agron Costarric 32:41
Chen, S-Y, Chien C-T, Baskin JM, and Baskin CC (2010) Storage behavior and changes in concentrations of abscisic acid and gibberellins during dormancy break and germination in seeds of Phellodendron amurense var. wilsonii (Rutaceae). Tree physiology 30(2):275–284
Azad MAK, Yokota S, Begum F, Yoshizawa N (2009) Plant regeneration through somatic embryogenesis of a medicinal plant, Phellodendron amurense Rupr. In-Vitro Cell Dev Biol Plant 45(4):441–449
Baskaran P, Jayabalan N (2009) In-vitro propagation of Psoralea corylifolia L. by somatic embryogenesis in cell suspension culture. Acta Physiol Plant 31(6):1119–1127
Chakraborty A, Bhattacharya D, Ghanta S, Chattopadhyay S (2010) An efficient protocol for in vitro regeneration of Podophyllum hexandrum, a critically endangered medicinal plant. Indian J Biotechnol 09(2):217
Kumar V, Moyo M, Van Staden J (2017) Somatic embryogenesis in Hypoxis hemerocallidea: an important African medicinal plant. S Afr J Bot 108:331–336
Khajuria AK, Hano C, Bisht NS (2021) Somatic embryogenesis and plant regeneration in Viola canescens Wall. Ex. Roxb.: an endangered Himalayan herb. Plants 10(4):761
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2023 The Author(s), under exclusive license to Springer Nature Switzerland AG
About this chapter
Cite this chapter
Lakshmi, M.V., Jeyaraj, S., Swapna, T.S. (2023). Conservation of Endangered Medicinal Plants by In Vitro Propagation Methods. In: Arunachalam, K., Yang, X., Puthanpura Sasidharan, S. (eds) Bioprospecting of Tropical Medicinal Plants. Springer, Cham. https://doi.org/10.1007/978-3-031-28780-0_42
Download citation
DOI: https://doi.org/10.1007/978-3-031-28780-0_42
Published:
Publisher Name: Springer, Cham
Print ISBN: 978-3-031-28779-4
Online ISBN: 978-3-031-28780-0
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)