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Whole-Body Chemiluminescence and Fluorescence Imaging of Inflammation

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Imaging Inflammation

Part of the book series: Progress in Inflammation Research ((PIR,volume 91))

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Abstract

This chapter focuses on the application of optical imaging methods to visualize inflammation in living animals. Optical imaging has several advantages as it operates without any involvement of ionizing radiation or strong magnetic fields. Optical imaging uses nonradioactive probes that produce chemiluminescent or fluorescent light signals in the visible, far-red, or near-infrared (NIR) range of the electromagnetic spectrum. To visualize inflammation at the tissue level, high molecular weight probes can be used to produce fluorescent contrast in the inflamed tissues by taking advantage of the enhanced permeability and retention (EPR) effect. In addition to this general, but rather less selective, approach, the chapter discusses the more specific and mechanistic imaging strategies that specifically target several unique biological aspects of inflammatory processes at the cellular and enzyme levels. These unique aspects include the inflammatory phagocytes that produce reactive oxygen species (ROS), and the tissue-remodeling proteases present in the inflamed tissues. Once activated, specific probes can produce visible or NIR luminescent signals that can be quantified for assessing inflammatory responses. Although visible light is subject to scattering and attenuation in the tissue, fluorescent probes that use NIR light sources have improved tissue penetration allowing generation of 3D tomographic images.

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Authors and Affiliations

  1. PerkinElmer, Inc., Hopkinton, MA, USA

    Jen-Chieh Tseng & Jeffrey D. Peterson

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  1. Jen-Chieh Tseng
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  2. Jeffrey D. Peterson
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Correspondence to Jen-Chieh Tseng .

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Editors and Affiliations

  1. Institute of Pharmaceutical Science, King#x2019;s College London, London, UK

    Francis Man

  2. Department of Medicine, University of California, San Francisco, San Francisco, CA, USA

    Simon J. Cleary

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Funding/Conflict of Interest: This work is sponsored by PerkinElmer Inc., and its R&D research division for developing advanced molecular imaging technologies. Both authors of this chapter (J.C. Tseng and J.D. Peterson) are employees of PerkinElmer Inc.

Ethical Approval: All animal research involved in this work followed the guidelines of PerkinElmer’s Institutional Animal Care and Use Committee (IACUC). A research protocol (#01-0112) was approved for this study.

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Tseng, JC., Peterson, J.D. (2023). Whole-Body Chemiluminescence and Fluorescence Imaging of Inflammation. In: Man, F., Cleary, S.J. (eds) Imaging Inflammation. Progress in Inflammation Research, vol 91. Springer, Cham. https://doi.org/10.1007/978-3-031-23661-7_5

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