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In Vitro Study of Specific Properties of Probiotic Strains for Effective and Personalized Probiotic Therapy

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Microbiome in 3P Medicine Strategies

Part of the book series: Advances in Predictive, Preventive and Personalised Medicine ((APPPM,volume 16))

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Abstract

Probiotics have tremendous potential to develop healthy diets, treatment and prevention. Investigation of in vitro cultural properties of health-promoting microorganisms like lactic acid bacteria (LAB) and Bifidobacteria is crucial to select probiotic strains for treatments based on gut microbiota modulation to justify individualized and personalized approach for nutrition and prevention of variety of diseases.

The studied strains of LAB and bifidobacteria did not form spores, were positively stained by Gram, grow on medium in a wide range of pH (1.0–9.0, optimum pH 5.5–6.5), were sensitive to wide range of antibiotics; and showed different resistance to gastric juice, bile and pancreatic enzymes.

The most resistant to antibiotics were L. rhamnosus LB-3 VK6 and L. delbrueckii LE VK8 strains. The most susceptible to gastric juice was L. plantarum LM VK7, which stopped its growth at 8% of gastric juice; L. acidophilus IMV B-7279, B. animalis VKL and B. animalis VKB strains were resistant even in the 100% concentration.

Strains L. acidophilus IMV В-7279, L. casei IMV В-7280, B. animalis VKL, B. animalis VKB, L. rhamnosus LB-3 VK6, L. delbrueckii LE VK8 and L. delbrueckii subsp. bulgaricus IMV В-7281 were resistant to pancreatic enzymes.

Adhesive properties of the strains according to AIM index were high in L. casei IMV В-7280, B. animalis VKL and B. animalis VKB; were moderate in L. delbrueckii subsp. bulgaricus IMV В-7281; and were low in L. acidophilus IMV В-7279, L. rhamnosus LB-3 VK6, L. delbrueckii LE VK8 and L. plantarum LM VK7.

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  1. Zabolotny Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, Kyiv, Ukraine

    Rostyslav V. Bubnov, Lidiia P. Babenko, Liudmyla M. Lazarenko, Victoria V. Mokrozub & Mykola Spivak

  2. Clinical Hospital ‘Pheophania’ of State Affairs Department, Kyiv, Ukraine

    Rostyslav V. Bubnov

  3. PJSC «SPC Diaproph-Med», Kyiv, Ukraine

    Mykola Spivak

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  1. Clinical Laboratory Diagnostics and Pharmacology, Uzhhorod National University & Ediens LLC, Uzhhorod, Ukraine

    Nadiya Boyko

  2. 3P Medicine, Radiation Oncology, Universitätsklinik Bonn, Bonn, Nordrhein-Westfalen, Germany

    Olga Golubnitschaja

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Bubnov, R.V., Babenko, L.P., Lazarenko, L.M., Mokrozub, V.V., Spivak, M. (2023). In Vitro Study of Specific Properties of Probiotic Strains for Effective and Personalized Probiotic Therapy. In: Boyko, N., Golubnitschaja, O. (eds) Microbiome in 3P Medicine Strategies. Advances in Predictive, Preventive and Personalised Medicine, vol 16. Springer, Cham. https://doi.org/10.1007/978-3-031-19564-8_13

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