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Structural Analysis of Oligosaccharides and Glycoconjugates Using NMR

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Glycobiology of the Nervous System

Part of the book series: Advances in Neurobiology ((NEUROBIOL,volume 29))

Abstract

Carbohydrate chains play critical roles in cellular recognition and subsequent signal transduction in the nervous system. Furthermore, gangliosides are targets for various amyloidogenic proteins associated with neurodegenerative disorders. To better understand the molecular mechanisms underlying these biological phenomena, atomic views are essential to delineate dynamic biomolecular interactions. Nuclear magnetic resonance (NMR) spectroscopy provides powerful tools for studying structures, dynamics, and interactions of biomolecules at the atomic level. This chapter describes the basics of solution NMR techniques and their applications to the analysis of 3D structures and interactions of glycoconjugates in the nervous system.

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Abbreviations

Aβ:

Amyloid β

DIS:

Deuterium-induced isotope shift

FID:

Free induction decay

HSQC:

Heteronuclear single-quantum coherence

MD:

Molecular dynamics

NMR :

Nuclear magnetic resonance

NOE:

Nuclear Overhauser effect

NOESY:

NOE spectroscopy

PCS:

Pseudocontact shift

PRE:

Paramagnetic relaxation enhancement

REMD:

Replica exchange MD

RF:

Radio frequency

STD:

Saturation transfer difference

TRNOE:

Transferred NOE

TROSY:

Transverse relaxation optimized spectroscopy

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Acknowledgments

This study was partly supported by JSPS/MEXT KAKENHI Grant-in-Aid for Scientific Research on Innovation Areas (JP20107004, JP25102008, JP25102001, and JP15K21708), Scientific Research (A) (JP24249002 and JP19H01017), Scientific Research (B) (JP16H04758 and JP19H03362), Scientific Research (C) (JP25460054), Challenging Exploratory, Research (JP26560451), and Young Scientists (B) (JP24750170) and the Japan Agency for Medical Research and Development, AMED (Project for utilizing glycans in the development of innovative drug discovery technologies).

Compliance with Ethics Requirements The authors declare that they have no conflict of interest and that they have used no human subjects in work cited that was done in their laboratory.

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Authors and Affiliations

  1. Division of Structural Biology, Institute of Molecular Biomembrane and Glycobiology, Tohoku Medical and Pharmaceutical University, Sendai, Japan

    Yoshiki Yamaguchi

  2. School of Materials Science, Japan Advanced Institute of Science and Technology, Nomi, Japan

    Takumi Yamaguchi

  3. Graduate School of Pharmaceutical Sciences, Nagoya City University, Mizuho-ku, Nagoya, Japan

    Takumi Yamaguchi & Koichi Kato

  4. Exploratory Research Center on Life and Living Systems and Institute for Molecular Science, Okazaki, Japan

    Koichi Kato

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  1. Yoshiki Yamaguchi
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Correspondence to Yoshiki Yamaguchi or Koichi Kato .

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Editors and Affiliations

  1. Biochemistry and Molecular Biology, The Pennsylvania State University, Hershey, PA, USA

    Cara-Lynne Schengrund

  2. Department of Neuroscience and Regenerative Medicine, Medical College of Georgia Augusta University, Augusta, GA, USA

    Robert K. Yu

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Yamaguchi, Y., Yamaguchi, T., Kato, K. (2023). Structural Analysis of Oligosaccharides and Glycoconjugates Using NMR. In: Schengrund, CL., Yu, R.K. (eds) Glycobiology of the Nervous System. Advances in Neurobiology, vol 29. Springer, Cham. https://doi.org/10.1007/978-3-031-12390-0_6

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