Abstract
In the process of fermentation, through which microbes obtain energy, they produce various enzymes like amylase, cellulase, etc. and break down complex organic substrates. Unlike aerobic respiration, where oxygen is the terminal electron acceptor or anaerobic respiration, where inorganic substances act as terminal electron acceptor, in fermentation, some endogenous organic compounds produced by the microbes act as terminal electron acceptor and recycle NADH back to NAD+. Microbes are thus used industrially for the production of many commercially important enzymes and organic acids. An advantage of using microbial fermentation for industrial production of enzymes is that many cheap substrates and waste materials can be used by the microbes to yield enzymes and other metabolites. Amylase enzyme production by the fungi Aspergillus niger using ammonium molasses medium as substrate offers an opportunity for entrepreneurship in view of growing demand for amylase enzyme by carbohydrate-related industries. Optimization of the process can help in making amylase production commercially competitive and address environmental issues like waste management by using agricultural wastes as substrates. Optimum physical parameters studied in this chapter found that pH of 6.5 at a temperature of 40 °C and 96 h of incubation yielded maximum amylase enzyme.
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Chakraborty, M.B., Patgiri, S.R., Sarma, M., Upadhyaya, H. (2022). Optimizing Physical Parameters for Amylase Production Using Aspergillus niger and Ammonium Molasses Medium. In: Shukla, A.C. (eds) Applied Mycology. Fungal Biology. Springer, Cham. https://doi.org/10.1007/978-3-030-90649-8_12
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