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Modeling the Early Steps of Ovarian Cancer Dissemination in an Organotypic Culture of the Human Peritoneal Cavity

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Ovarian Cancer: Molecular & Diagnostic Imaging and Treatment Strategies

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 1330))

Abstract

The majority of ovarian cancer patients present clinically with wide-spread metastases throughout the peritoneal cavity, metastasizing to the mesothelium-lined peritoneum and visceral adipose depots within the abdomen. This unique metastatic tumor microenvironment is comprised of multiple cell types, including mesothelial cells, fibroblasts, adipocytes, macrophages, neutrophils, and T lymphocytes. Modeling advancements, including complex 3D systems and organoids, coupled with 2D cocultures, in vivo mouse models, and ex vivo human tissue cultures have greatly enhanced our understanding of the tumor-stroma interactions that are required for successful metastasis of ovarian cancer cells. However, advanced multifaceted model systems that incorporate frequency and spatial distribution of all cell types present in the tumor microenvironment of ovarian cancer are needed to enhance our knowledge of ovarian cancer biology in order to identify methods for preventing and treating metastatic disease. This review highlights the utility of recently developed modeling approaches, summarizes some of the resulting progress using these techniques, and suggests how these strategies may be implemented to elucidate signaling processes among cell types of the tumor microenvironment that promote ovarian cancer metastasis.

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Authors and Affiliations

  1. College of Pharmacy, Roosevelt University, Chicago, IL, USA

    Peter C. Hart

  2. Department of Obstetrics and Gynecology, Gordon Center of Integrative Sciences, W116, University of Chicago, Chicago, IL, USA

    Preety Bajwa & Hilary A. Kenny

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  1. Dept. Veterinary Pathobiology, University of Missouri-Columbia Dept. Veterinary Pathobiology, COLUMBIA, MO, USA

    Heide Schatten

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Hart, P.C., Bajwa, P., Kenny, H.A. (2021). Modeling the Early Steps of Ovarian Cancer Dissemination in an Organotypic Culture of the Human Peritoneal Cavity. In: Schatten, H. (eds) Ovarian Cancer: Molecular & Diagnostic Imaging and Treatment Strategies. Advances in Experimental Medicine and Biology, vol 1330. Springer, Cham. https://doi.org/10.1007/978-3-030-73359-9_5

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