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In Vivo and In Vitro Properties of Ovarian Cancer Cells

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Ovarian Cancer

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1049))

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Abstract

Specific biological properties of ovarian cancer cells can be modeled and studied using in vitro experiments. Any experimental setting can closely reflect some aspects of the native conditions; however, parameters that differ from in vivo aspects must be considered. Familiarity with existing and well-established, as well as new, cell culture techniques provides a basis for correct experimental design and production of reliable scientific results. This chapter presents a short comparative review of the techniques used for cell culture establishment and maintenance of ovarian cancer cells, as well as laboratory methods used to characterize malignant features of these cells, including the epithelial–mesechymal transition, cell motility and invasiveness, resistance to detachment-induced apoptosis, and stem cell content.

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Authors and Affiliations

  1. Laboratory of Oncoendocrinology, N.N. Petrov Institute of Oncology, St. Petersburg, Russia

    Anastasia Malek

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  1. Anastasia Malek
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Editors and Affiliations

  1. Group of Experimental Therapeutics, Federal State Institution N.N.Petrov Res Institute of Oncology, St. Petersburg, Russia

    Anastasia Malek

  2. Labs of Molecular Tumor Pathology & Func, Charité - Universitatsmedizin Berlin, Berlin, Germany

    Oleg Tchernitsa

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Malek, A. (2013). In Vivo and In Vitro Properties of Ovarian Cancer Cells. In: Malek, A., Tchernitsa, O. (eds) Ovarian Cancer. Methods in Molecular Biology, vol 1049. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-547-7_23

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  • DOI: https://doi.org/10.1007/978-1-62703-547-7_23

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-546-0

  • Online ISBN: 978-1-62703-547-7

  • eBook Packages: Springer Protocols

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