Abstract
It is generally accepted that the detection limit of bacterial cells using biosensors is one cell. The present chapter describes the specific visualization of bacterial nanofragments using atomic force microscopy (AFM ). The development of new sensitive biosensor methods for the analysis of bacteria is a very important area in biotechnology. AFM is a form of scanning probe microscopy where a cantilever with a very sharp tip is periodically scanned across special surface with immobilized sample. AFM can also be used to measure forces between the tip and sample. The high-resolution imaging of living object is easily possible using AFM. AFM is a powerful, multifunctional imaging platform that allows to visualize and manipulate with biological samples from single molecules to living cells. An artificial nose is unique system for non-contact method for microbial detection. Using this approach, it is possible to detect bacterial “footstep,” even after removing bacteria from the assay medium. These two approaches can be used for detection of less than one bacterial cell.
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Abbreviations
- AFM:
-
Atomic force microscopy
- CFU:
-
Colony-forming unit
- LB:
-
Lysogeny broth
- OD:
-
Optical density
- VOCs:
-
Volatile organic components
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Acknowledgments
The work was supported by Rospotrebnadzor. F.S.Y. and G.P.B. were supported by the State Аssignment 0104-2019-0024 to Research Center of Biotechnology RAS. The authors wish to thank E.V. Dubrovin and D.S. Ignatov for the help.
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Ignatov, S.G., Voloshin, A.G., Bachurina, G.P., Filippovich, S.Y., Dyatlov, I.A. (2021). Is It Possible to Detect Less Than One Bacterial Cell?. In: Rai, M., Reshetilov, A., Plekhanova, Y., Ingle, A.P. (eds) Macro, Micro, and Nano-Biosensors. Springer, Cham. https://doi.org/10.1007/978-3-030-55490-3_4
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