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Sperm Processing and Selection

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Male Infertility

Abstract

Assisted reproductive technology (ART) use sperm sorting methods to select viable sperm from semen samples. Conventional sperm sorting techniques currently use swim-up and density gradient centrifugation. These methods use multiple centrifugation steps, which have been shown to generate reactive oxygen species (ROS) that decrease DNA integrity and damage sperm. Newer technologies, such as motile sperm organelle morphology examination (MSOME), electrophoresis and magnetically activated cell sorting, and microfluidics, eliminate the centrifugation steps, reduce oxidative stress, and improve the selection of sperm with higher DNA integrity, normal morphology, and motility and may help improve assisted reproductive outcomes. In this chapter, we discuss some centrifugation- and non-centrifugation-based techniques for sperm selection and their effect on sperm quality and ART outcomes.

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  1. American Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH, USA

    Rakesh Sharma & Ashok Agarwal

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  1. Rakesh Sharma
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  2. Ashok Agarwal
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Correspondence to Ashok Agarwal .

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  1. PUR Clinic, University of Central Florida (UCF), Clermont, FL, USA

    Sijo J. Parekattil

  2. ANDROFERT and State University of Campinas (UNICAMP), Campinas, Brazil

    Sandro C. Esteves

  3. Cleveland Clinic Lerner College of Medicine, Cleveland, OH, USA

    Ashok Agarwal

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Sharma, R., Agarwal, A. (2020). Sperm Processing and Selection. In: Parekattil, S., Esteves, S., Agarwal, A. (eds) Male Infertility. Springer, Cham. https://doi.org/10.1007/978-3-030-32300-4_52

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