Abstract
Estimates of decomposition and consumption rates of plant litter generally rely on the incubation of a known quantity of leaves. However, the chemical composition of natural plant material is highly variable among species and biogeographic regions, and cannot be easily manipulated for experimental purposes. This chapter describes a method to overcome these constraints using decomposition and consumption tablets (DECOTABs). DECOTABs are easy-to-prepare agar-based pellets (agar 25% dry mass) with the remaining 75% being organic matter (OM) at choice. In the standard DECOTAB, the 75% OM consists entirely of cellulose powder. For specific DECOTABs, part or all of the cellulose can be replaced to vary OM composition, for example, by adding particulate organic matter, ground plant litter or specific substances such as polyunsaturated fatty acids (PUFAs). This makes the DECOTABs highly standardized, inexpensive and adjustable, enabling extensive manipulation of chemical composition. Proposed only recently, DECOTABs have already provided insight into the importance of environmental conditions, detritivore community composition and litter quality in controlling decomposition and consumption rates.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Bjelke, U. (2005). Processing of leaf matter by lake-dwelling shredders at low oxygen concentrations. Hydrobiologia, 539, 93–98.
Boulton, A. J., & Boon, P. I. (1991). A review of methodology used to measure leaf litter decomposition in lotic environments: Time to turn over an old leaf? Marine and Freshwater Research, 42, 1–43.
Dang, C. K., Schindler, M., Chauvet, E., & Gessner, M. O. (2009). Temperature oscillation coupled with fungal community shifts can modulate warming effects on litter decomposition. Ecology, 90, 122–131.
Danger, M., Arce Funck, J., Devin, S., Heberle, J., & Felten, V. (2013). Phosphorus content in detritus controls life-history traits of a detritivore. Functional Ecology, 27, 807–815.
Gessner, M. O. (1991). Differences in processing dynamics of fresh and dried leaf litter in a stream ecosystem. Freshwater Biology, 26, 387–398.
González, J. M., & Graça, M. A. S. (2003). Conversion of leaf litter to secondary production by a shredding caddis-fly. Freshwater Biology, 48, 1578–1592.
Graça, M. A. S., & Poquet, J. M. (2014). Do climate and soil influence phenotypic variability in leaf litter, microbial decomposition and shredder consumption? Oecologia, 174, 1021–1032.
Hines, J., Reyes, M., Mozder, T. J., & Gessner, M. O. (2014). Genotypic trait variation modifies effects of climate warming and nitrogen deposition on litter mass loss and microbial respiration. Global Change Biology, 20, 3780–3789.
Hunting, E. R., Vonk, J. A., Musters, C. J. M., Kraak, M. H. S., & Vijver, M. G. (2016). Effects of agricultural practices on organic matter degradation in ditches. Scientific Reports, 6, 21474.
Kampfraath, A. A., Hunting, E. R., Mulder, C., Breure, A. M., Gessner, M. O., Kraak, M. H. S., & Admiraal, W. (2012). DECOTAB: A multipurpose standard substrate to assess effects of litter quality on microbial decomposition and invertebrate consumption. Freshwater Science, 31, 1156–1162.
Lecerf, A., & Chauvet, E. (2008). Intraspecific variability in leaf traits strongly affects alder leaf decomposition in a stream. Basic and Applied Ecology, 9, 598–605.
LeRoy, C. J., Whitham, T. G., Keim, P., & Marks, J. C. (2006). Plant genes link forests and streams. Ecology, 87, 255–261.
Pedersen, J. C. (1992). Natamycin as a fungicide in agar media. Applied and Environmental Microbiology, 58, 1064–1066.
Rader, R. B., McArthur, J. V., & Aho, J. M. (1994). Relative importance of mechanisms determining decomposition in a southeastern blackwater stream. American Midland Naturalist, 132, 19–31.
Sariyildiz, T., & Anderson, J. M. (2003). Decomposition of sun and shade leaves from three deciduous tree species, as affected by their chemical composition. Biology and Fertility of Soils, 37, 137–146.
Suberkropp, K., Godshalk, G. L., & Klug, M. J. (1976). Changes in the chemical composition of leaves during processing in a woodland stream. Ecology, 57, 720–727.
Swan, C. M., & Palmer, M. A. (2006). Composition of speciose leaf litter alters stream detritivore growth, feeding activity and leaf breakdown. Oecologia, 147, 469–478.
Talbot, J. M., & Treseder, K. K. (2012). Interactions among lignin, cellulose, and nitrogen drive litter chemistry–decay relationships. Ecology, 93, 345–354.
Tiegs, S. D., Langhans, S. D., Tockner, K., & Gessner, M. O. (2007). Cotton strips as a leaf surrogate to measure decomposition in river floodplain habitats. Journal of the North American Benthological Society, 26, 70–77.
Van der Lee, G. H., Kraak, M. H. S., Verdonschot, R. C. M., Vonk, A. J., & Verdonschot, P. F. M. (2017). Oxygen drives benthic-pelagic decomposition pathways in shallow wetlands. Scientific Reports, 7, 15051.
Van Ginneken, V. J. T., Helsper, J. P. F. G., De Visser, W., Van Keulen, H., & Brandenburg, W. A. (2011). Polyunsaturated fatty acids in various macroalgal species from north Atlantic and tropical seas. Lipids in Health and Disease, 10, 104.
Vonk, J. A., Van Kuijk, B. F., Van Beusekom, M., Hunting, E. R., & Kraak, M. H. S. (2016). The significance of linoleic acid in food sources for detritivorous benthic invertebrates. Scientific Reports, 6, 35785.
Webster, J. R., & Benfield, E. F. (1986). Vascular plant breakdown in freshwater ecosystems. Annual Review of Ecology and Systematics, 17, 567–594.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2020 Springer Nature Switzerland AG
About this chapter
Cite this chapter
Van der Lee, G.H., Hunting, E.R., Vonk, J.A., Kraak, M.H.S. (2020). Decomposition and Consumption Tablets (DECOTABs). In: Bärlocher, F., Gessner, M., Graça, M. (eds) Methods to Study Litter Decomposition. Springer, Cham. https://doi.org/10.1007/978-3-030-30515-4_57
Download citation
DOI: https://doi.org/10.1007/978-3-030-30515-4_57
Published:
Publisher Name: Springer, Cham
Print ISBN: 978-3-030-30514-7
Online ISBN: 978-3-030-30515-4
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)