Abstract
Cell-free protein synthesis is advantageous for membrane protein production because of the potential lethality of membrane proteins synthesized in cell-based systems. Additionally the ability to adapt the membrane composition for each membrane protein increases the likelihood of functional expression. In this chapter we describe methods for membrane protein production by the cell-free protein synthesis system assisted by detergents and lipids.
An erratum to this chapter can be found at http://dx.doi.org/10.1007/978-1-62703-782-2_22
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Acknowledgements
We thank Dr. Noboru Ohsawa for comments on the manuscript. This work was supported by the RIKEN Structural Genomics/Proteomics Initiative (RSGI), the National Project on Protein Structural and Functional Analyses, and the Targeted Proteins Research Program (TPRP) of the Ministry of Education, Culture, Sports, Science and Technology of Japan.
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Kimura-Soyema, T., Shirouzu, M., Yokoyama, S. (2014). Cell-Free Membrane Protein Expression. In: Alexandrov, K., Johnston, W. (eds) Cell-Free Protein Synthesis. Methods in Molecular Biology, vol 1118. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-782-2_18
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DOI: https://doi.org/10.1007/978-1-62703-782-2_18
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-781-5
Online ISBN: 978-1-62703-782-2
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