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Scalable Transient Protein Expression

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Animal Cell Biotechnology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1104))

Abstract

Transient transfection is a well-established method to rapidly express recombinant proteins from mammalian cells. Accelerating activity in biotherapeutic drug development, demand for protein-based reagents, vaccine research, and large initiatives in structural and functional studies of proteins have propelled the need to generate moderate to high amounts of recombinant proteins and other macromolecules in a flexible and rapid manner. Progress over the last 10–15 years has demonstrated that transient transfections can be reliably and readily scaled up to handle milliliters to tens of liters of cells in suspension culture and obtain milligrams to grams of recombinant protein in a process that requires only days to weeks. This review will summarize developments in this field, properties of the components of a transient expression system that enable maximal protein production, and detailed protocols for this application.

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Author information

Authors and Affiliations

  1. Life Technologies, Carlsbad, CA, USA

    Henry C. Chiou, Sanjay Vasu, Chao Yan Liu, Isabel Cisneros, Meredith B. Jones & Jonathan F. Zmuda

Authors
  1. Henry C. Chiou
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  2. Sanjay Vasu
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  3. Chao Yan Liu
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  4. Isabel Cisneros
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  5. Meredith B. Jones
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  6. Jonathan F. Zmuda
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Editor information

Editors and Affiliations

  1. Bioprozess- und Biosystemtechnik, Technische Universität Hamburg-Harburg, Hamburg, Germany

    Ralf Pörtner

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© 2014 Springer Science+Business Media, LLC

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Chiou, H.C., Vasu, S., Liu, C.Y., Cisneros, I., Jones, M.B., Zmuda, J.F. (2014). Scalable Transient Protein Expression. In: Pörtner, R. (eds) Animal Cell Biotechnology. Methods in Molecular Biology, vol 1104. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-733-4_4

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  • DOI: https://doi.org/10.1007/978-1-62703-733-4_4

  • Published:

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-732-7

  • Online ISBN: 978-1-62703-733-4

  • eBook Packages: Springer Protocols

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