Abstract
Immunoblot-coupled proteomics based on two-dimensional polyacrylamide gel electrophoresis (2D-PAGE), namely, immunoproteomics, has been used for comprehensive identification of food allergens, because it is a simple and inexpensive tool for rapid identification of several IgE-binding proteins. In this section, we describe our protocols for identification of food allergens using immunoproteomics and discuss a few technical points in detail.
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This work was supported by a grant from the Ministry of Health, Labour and Welfare, Japan.
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Nakamura, R., Teshima, R. (2014). Immunoproteomics Analysis of Food Allergens. In: Jorrin-Novo, J., Komatsu, S., Weckwerth, W., Wienkoop, S. (eds) Plant Proteomics. Methods in Molecular Biology, vol 1072. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-631-3_50
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DOI: https://doi.org/10.1007/978-1-62703-631-3_50
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