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Isolation and Culture of Pig Epiblast Stem Cells

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Epiblast Stem Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1074))

Abstract

Regulation of early development can be directly interrogated in the embryo or can be studied in cultured cells isolated in the laboratory. New understanding of the developmental stages and the signalling requirements of the pig embryo have enabled the development of improved protocols for the derivation of pluripotent cells from early epiblasts. Here, we provide a detailed step-by-step description of the critical parameters required for isolation and establishment of these cells and how they can be used to study their developmental properties.

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Author information

Authors and Affiliations

  1. Division of Animal Sciences, School of Biosciences, University of Nottingham, Loughborough, UK

    Aida Rodriguez, David A. Contreras & Ramiro Alberio

Authors
  1. Aida Rodriguez
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  2. David A. Contreras
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  3. Ramiro Alberio
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Editor information

Editors and Affiliations

  1. School of Biosciences Rm 223 South Lab, University of Nottingham, Loughborough, United Kingdom

    Ramiro Alberio

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Rodriguez, A., Contreras, D.A., Alberio, R. (2013). Isolation and Culture of Pig Epiblast Stem Cells. In: Alberio, R. (eds) Epiblast Stem Cells. Methods in Molecular Biology, vol 1074. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-628-3_8

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  • DOI: https://doi.org/10.1007/978-1-62703-628-3_8

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-627-6

  • Online ISBN: 978-1-62703-628-3

  • eBook Packages: Springer Protocols

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