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Establishment of Mouse Epiblast Stem Cells

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Epiblast Stem Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2490))

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Abstract

Epiblast stem cells are made from the epiblast of mouse post-implantation embryo. They have been critical in the understanding of mammalian pluripotent stem cells as they share similar properties, such as their incapability to contribute to the formation of an embryo after injection into blastocyst. The epiblast stem cells (EpiSC) have delineated a novel status of pluripotency called “primed.” How to establish EpiSC from mouse embryo is described in detail in this chapter.

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References

  1. Tam PPL, Loebel DAF (2007) Gene function in mouse embryogenesis: get set for gastrulation. Nat Rev Genet 8:368–381. https://doi.org/10.1038/nrg2084

    Article  CAS  PubMed  Google Scholar 

  2. Evans MJ, Kaufman MH (1981) Establishment in culture of pluripotential cells from mouse embryos. Nature 292:154–156

    Article  CAS  Google Scholar 

  3. Martin GR (1981) Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by Teratocarcinoma stem cells. Proc Natl Acad Sci U S A 78:7634–7638. https://doi.org/10.1073/pnas.78.12.7634

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Brons IG, Smithers LE, Trotter MW, Rugg-Gunn P, Sun B, de Sousa C, Lopes SM, Howlett SK, Clarkson A, Ahrlund-Richter L, Pedersen RA, Vallier L (2007) Derivation of pluripotent epiblast stem cells from mammalian embryos. Nature 448:191–195. https://doi.org/10.1038/nature05950

    Article  CAS  PubMed  Google Scholar 

  5. Tesar PJ, Chenoweth JG, Brook FA, Davies TJ, Evans EP, Mack DL, Gardner RL, McKay RD (2007) New cell lines from mouse epiblast share defining features with human embryonic stem cells. Nature 448:196–199. https://doi.org/10.1038/nature05972

    Article  CAS  PubMed  Google Scholar 

  6. Bradley A, Evans M, Kaufman MH, Robertson E (1984) Formation of germ-line chimaeras from embryo-derived teratocarcinoma cell lines. Nature 309:255–256

    Article  CAS  Google Scholar 

  7. Kojima Y, Tam OH, Tam PPL (2014) Timing of developmental events in the early mouse embryo. Semin Cell Dev Biol 34:65–75. https://doi.org/10.1016/j.semcdb.2014.06.010

    Article  CAS  PubMed  Google Scholar 

  8. Kaufman-Francis K, Goh HN, Kojima Y, Studdert JB, Jones V, Power MD, Wilkie E, Teber E, Loebel DAF, Tam PPL (2014) Differential response of epiblast stem cells to nodal and Activin signalling: a paradigm of early endoderm development in the embryo. Philos Trans R Soc Lond Ser B Biol Sci 369(1657):20130550. https://doi.org/10.1098/rstb.2013.0550

    Article  CAS  Google Scholar 

  9. Kojima Y, Kaufman-Francis K, Studdert JB, Steiner KA, Power MD, Loebel DAF, Jones V, Hor A, de Alencastro G, Logan GJ, Teber ET, Tam OH, Stutz MD, Alexander IE, Pickett HA, Tam PPL (2014) The transcriptional and functional properties of mouse epiblast stem cells resemble the anterior primitive streak. Cell Stem Cell 14:107–120. https://doi.org/10.1016/j.stem.2013.09.014

    Article  CAS  PubMed  Google Scholar 

  10. Hayashi K, Ohta H, Kurimoto K, Aramaki S, Saitou M (2011) Reconstitution of the mouse germ cell specification pathway in culture by pluripotent stem cells. Cell 146:519–532. https://doi.org/10.1016/j.cell.2011.06.052

    Article  CAS  PubMed  Google Scholar 

  11. Osteil P, Studdert J, Wilkie E, Fossat N, Tam PPL (2015) Generation of genome-edited mouse epiblast stem cells via a detour through ES cell-chimeras. Differentiation 91:119–125. https://doi.org/10.1016/j.diff.2015.10.004

    Article  CAS  PubMed  Google Scholar 

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Correspondence to Pierre Osteil .

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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Osteil, P. (2022). Establishment of Mouse Epiblast Stem Cells. In: Osteil, P. (eds) Epiblast Stem Cells. Methods in Molecular Biology, vol 2490. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2281-0_1

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  • DOI: https://doi.org/10.1007/978-1-0716-2281-0_1

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2280-3

  • Online ISBN: 978-1-0716-2281-0

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