Abstract
Here, we describe methods to evaluate the ability of small molecules inhibitors of TNAP and PHOSPHO1 in preventing mineralization of primary cultures of murine vascular smooth muscle cells. The procedures are also applicable to primary cultures of calvarial osteoblasts. These cell-based assays are used to complement kinetic testing during structure–activity relationship studies aimed at improving scaffolds in the generation of pharmaceuticals for the treatment for medial vascular calcification.
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Acknowledgments
This work was supported in part by grant R01AR053102 and R01AR047908 from the National Institute of Arthritis and Musculoskeletal Diseases (NIAMS), and American Recovery and Reinvestment Act (ARRA) Challenge grant RC1HL10899 from the National Heart, Lung, and Blood Institute (NHLB), National Institutes of Health (NIH), USA, and Institute Strategic Program Grant Funding from the Biotechnology and Biological Sciences Research Council.
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Kiffer-Moreira, T., Narisawa, S. (2013). The Use of Tissue-Nonspecific Alkaline Phosphatase (TNAP) and PHOSPHO1 Inhibitors to Affect Mineralization by Cultured Cells. In: Millán, J. (eds) Phosphatase Modulators. Methods in Molecular Biology, vol 1053. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-562-0_8
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DOI: https://doi.org/10.1007/978-1-62703-562-0_8
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-561-3
Online ISBN: 978-1-62703-562-0
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